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First report of canna yellow streak virus in Iran | ||
| Iran Agricultural Research | ||
| مقاله 12، دوره 41، شماره 2، 2023، صفحه 159-163 اصل مقاله (945.82 K) | ||
| نوع مقاله: Note | ||
| شناسه دیجیتال (DOI): 10.22099/iar.2023.47324.1540 | ||
| نویسندگان | ||
| M Salehzadeh1؛ A Afsharifar* 1؛ S Dehghanpour Farashah2 | ||
| 1Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran | ||
| 2Agriculture Department, Payame Noor University, Tehran, I. R. Iran | ||
| چکیده | ||
| Canna indica (canna) a species in the Cannaceae family, is a perennial ornamental plant widely used in landscape designing. Different species of plant viruses have been reported which infect this plant and act as a significant threat to canna plants, leading to a range of symptoms and a decrease in their decorative values, as well as lower quality propagated materials and significant financial losses. Canna cultivars that are extensively cultivated for their ornamental values are susceptible to a recently discovered Potyvirus known as canna yellow streak virus (CaYSV). This virus has infected many canna cultivars found in gardens, leading to significant impact and concern in horticultural settings. During a survey conducted in the summer of 2022, severe veinal chlorosis and veinal streaking symptoms were observed on the leaves of canna plants in the green space of Yazd City, Iran. Total genomic RNA was extracted from symptomatic leaves of 20 canna samples and one symptomless sample (negative control), and subjected to RT-PCR using a potyvirus degenerate primer pair (NIb3R, NIb2F). RT-PCR resulted in the amplification of a DNA fragment with the expected size of approximately 350 bp in all of the symptomatic samples, whereas no DNA fragment was obtained from a symptomless plant. The amplified DNA fragment was subjected to the Sanger sequencing method, and its size was confirmed to be exactly 350 bp. Sequence analysis of the nucleotide sequence of this amplicon with the same region of other corresponding CaYSV isolates in the GenBank revealed that the Iranian CaYSV isolate was the most similar (99.05%) to an isolate from Russia (Acc. No. MG_545919.1) and the less similar (89.59%) to a CaYSV isolate from United Kingdom (Acc. No. EF_466139.1). | ||
| کلیدواژهها | ||
| Degenerate primers؛ Green space؛ Potyvirus؛ Yazd | ||
| مراجع | ||
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Alexandre, M. A. V., Duarte, L. M. L., Chaves, A. L. R., Ramos, A. F., Harakava, R., & Kitajima, E. W. (2017). Canna paniculata as natural host of canna yellow streak virus in Brazil. Australasian Plant Disease Notes, 38, Notes 12. https://doi.org/10.1007/s13314-017-0264-3 Andrade‐Mahecha, M. M., Tapia‐Blácido, D. R., & Menegalli, F. C. (2012). Physical–chemical, thermal, and functional properties of achira (Canna indica L.) flour and starch from different geographical origin. Starch‐Stärke, 64(5), 348-358. https://doi.org/10.1002/star.201100149 Beemster, A. B. R. (1982). Handbook of plant virus infections. Comparative diagnosis. Netherlands Journal of Plant Pathology, 88, 216–217. https://doi.org/10.1007/BF02140885 Castillo, B. S., Yarwood, C. E., & Gold, A. H. (1956). Canna-mosaic virus. Plant Disease Reporter, 40, 169-172. DOI 10.1007/s13314-012-0062-x Chauhan, R. P., Hamon, H. F., Rajakaruna, P., Webb, M. A., Payton, M., & Verchot, J. (2015). Reliable detection for Bean yellow mosaic virus, Canna yellow streak virus, and Canna yellow mottle virus in canna varieties with red foliage. Plant Disease, 99(2), 188-194. https://doi.org/10.1094/PDIS-05-14-0538-RE Deng, D., McGrath, P. F., Robinson, D. J., & Harrison, B. D. (1994). Detection and differentiation of whitefly‐transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of Applied Biology, 125(2), 327-336. https://doi.org/10.1111/j.1744-7348.1994.tb04973.x Hollings, M., & STONE, O. M. (1973). Some properties of chrysanthemum stunt, a virus with the characteristics of an uncoated ribonucleic acid. Annals of Applied Biology, 74(3), 333-348. https://doi.org/10.1111/j.1744-7348.1973.tb07754.x Gibbs, A., & Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. https://doi.org/10.1016/S0166-0934(96)02103-9 Kumari, A., Kaur, C., Kumar, S., Chauhan, P. S., & Raj, S. K. (2021). Current status of three virus genera (Badnavirus, Cucumovirus, and Potyvirus) in Canna Species in India. In Raj, S. K., Gaur, R. K., & Yin, Z. (Eds). Virus Diseases of Ornamental Plants: Characterization, Identification, Diagnosis and Management (pp 117-126). Singapore: Springer. https://doi.org/10.1007/978-981-16-3919-7_6 Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R., & Li, F. (2018). Detection of to bamoviruses by RT-PCR using a novel pair of degenerate primers. Journal of Virological Methods, 259, 122-128. https://doi.org/10.1016/j.jviromet.2018.06.012 Li, Y., Xia, F., Wang, Y., Yan, C., Jia, A., & Zhang, Y. (2019). Characterization of a highly divergent Sugarcane mosaic virus from Canna indica L. by deep sequencing. BMC Microbiology , 19, 1-8. https://doi.org/10.1186/s12866-019-1636-y Lockhart, B. E. L. (1988). Occurrence of Canna yellow mottle virus in North America. In VII International Symposium on Virus Diseases of Ornamental Plants 234 (pp. 69-72). https://doi.org/10.17660/ActaHortic.1988.234.7 Mitrofanova, I. V., Zakubanskiy, A. V., & Mitrofanova, O. V. (2018). Viruses infecting main ornamental plants: An overview. Ornamental Horticulture, 24, 95-102. https://doi.org/10.14295/oh.v24i2.1199 Monger, W. A., Harju, V., Skelton, A., Seal, S. E., & Mumford, R. A. (2007). Canna yellow streak virus: A new potyvirus associated with severe streaking symptoms in canna. Archives of Virology, 152(8), 1527-1530. https://doi.org/10.1007/s00705-007-0977-2 Punsasi, R., Mark, P., & Jeanmarie, V. (2015). Reliable detection for bean yellow mosaic virus, Canna yellow streak virus, and Canna yellow mottle virus in Canna varieties with red foliage. https://doi.org/10.1094/PDIS-05-14-0538-RE Rajakaruna, P., Shafiekhani, M., Kim, T., Payton, M., Chauhan, R., & Verchot, J. (2014). Production of discernible disease phenotypes in Canna by five plant viruses belonging to the genera Potyvirus, Cucumovirus and Badnavirus. Plant Pathology, 63(4), 821-830. https://doi.org/10.1111/ppa.12169 Rizos, H., Gunn, L. V., Pares, R. D., & Gillings, M. R. (1992). Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology, 73, 2099-2103. https://doi.org/10.1099/0022-1317-73-8-2099 Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease, 77(4), 340-347. https://doi.org/10.1094/pd-77-0340 Yamashita, S., Natsuaki, T., Doi, Y., & Yora, K. (1985). Canna yellow mottle virus, a non-enveloped small bacilliform virus in Canna sp. Japanese Journal of Phytopathology, 51(5), 642-646. https://doi.org/10.3186/jjphytopath.51.642 Zakubanskiy, A. V., Mitrofanova, I. V., & Chirkov, S. N. (2017). Molecular characterization of viruses infecting canna in Russia. European Journal of Plant Pathology , 149, 923-931. https://doi.org/10.1007/s10658-017-1241-6
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