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Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. (1402). Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. , 12(2), 87-94. doi: 10.22099/mbrc.2023.47151.1821
Ehsan Zoaktafi; Hassan Sharifiyazdi; Nooshin Derakhshandeh; Farnoosh Bakhshaei-shahrbabaki. "Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations". , 12, 2, 1402, 87-94. doi: 10.22099/mbrc.2023.47151.1821
Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. (1402). 'Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations', , 12(2), pp. 87-94. doi: 10.22099/mbrc.2023.47151.1821
Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. , 1402; 12(2): 87-94. doi: 10.22099/mbrc.2023.47151.1821

Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations

Molecular Biology Research Communications
دوره 12، شماره 2، 2023، صفحه 87-94    اصل مقاله (256.47 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.47151.1821
نویسندگان
Ehsan Zoaktafi؛ Hassan Sharifiyazdi؛ Nooshin Derakhshandeh* ؛ Farnoosh Bakhshaei-shahrbabaki
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده
Canine hepatozoonosis is a tick-transmitted apicomplexan infection caused by two species of Hepatozoon, H. canis, and H. americanum. The present research aimed at detection of Hepatozoon spp. in dogs and its effects on hematological alterations. Blood samples were taken from 108 dogs to assess Hepatozoon spp. Phylogenetic analysis was performed based on the 18S rDNA marker by PCR assay and Giemsa-stained blood smear examination. Of the 108 blood samples of dogs tested in the present study, eight (7.40%, 95% CI: 3.25-14.07%) were positive by the Hepatozoon-specific PCR assay. However, in the microscopic examination, only one sample (0.93%) was positive. All of the sequenced samples were H. canis. The Hepatozoon sequences obtained from PCR amplicons in the canine-positive cases exhibited 100% similarity to each other and 98.47-100% similarity to other relevant sequences in GenBank. These findings represent the first molecular evidence of H. canis in dog populations in South Iran. Furthermore, according to the hematological analysis, significantly higher average numbers of neutrophils and lymphocytes were found in the infected group compared to the non-infected dogs. In this study, no statistically significant connection (P<0.05) was observed between H. canis infection and the examined risk factors. 
کلیدواژه‌ها
Hepatozoon canis؛ Molecular detection؛ Dog؛ Hematological analysis
مراجع
  1. Modrý D, Beck R, Hrazdilova K, Baneth G. A review of methods for detection of Hepatozoon infection in carnivores and arthropod vectors. Vector Borne Zoonotic Dis 2017; 17:66-72.
  2. Johnson EM, Allen KE, Panciera RJ, Ewing SA, Little SE. Experimental transmission of Hepatozoon americanum to New Zealand White rabbits (Oryctolagus cuniculus) and infectivity of cystozoites for a dog. Vet Parasitol 2009;164:162-166.
  3. Díaz-Sánchez AA, Hofmann-Lehmann R, Meli ML, Roblejo-Arias L, Fonseca-Rodríguez O, Castillo AP, Cañizares EV, Rivero EL, Chilton NB, Corona-Gonzalez B. Molecular detection and characterization of Hepatozoon canis in stray dogs from Cuba. Parasitol Int 2021;80:102200.
  4. Khoshnegah J, Mohri M, Movassaghi AR, Kazemi Mehrjerdi H. The first report of Hepatozoon canis infection of a dog in Iran. Comp Clin Path 2009;18:455-458.
  5. Criado-Fornelio A, Rey-Valeiron C, Buling A, Barba-Carretero JC, Jefferies R, Irwin P. New advances in molecular epizootiology of canine hematic protozoa from Venezuela, Thailand and Spain. Vet Parasitol 2007;144:261-269.
  6. Allen KE, Little SE, Johnson EM, Hostetler J, Panciera RJ, Ewing SA. Treatment of Hepatozoon americanum infection: review of the literature and experimental evaluation of efficacy. Vet Ther 2010;11:E1-E8.
  7. Criado-Fornelio A, Martinez-Marcos A, Buling-Sarana A, Barba-Carretero JC. Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe: part I. Epizootiological aspects. Vet Parasitol 2003;113:189-201.
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-2729.
  9. Rahmani Amoli A, Khoshnegah J, Razmi G. A preliminary parasitological survey of hepatozoon spp. Infection in dogs in mashhad, iran. Iran J Parasitol 2012;7:99-103.
  10. O’dwyer LH, Massard CL, de Souza JCP. Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil. Vet Parasitol 2001;94:143-150.
  11. Erol U, Altay K, Duran ATAŞ A, Özkan E, Faruk Şahin O. Molecular Prevalence of Canine Hepatozoonosis in Owned-Dogs in Central Part of Turkey. Isr J Vet Med, 2021;76:71-76.
  12. Dalimi A, Jameie F, Mohammadiha A, Barati M, Molaei S. Molecular detection of Hepatozoon canis in dogs of Ardabil Province, Northwest of Iran. Arch Razi Inst 2017;72: 197-201.
  13. Soltani R, Dalimi A. A molecular study on Hepatozoon canis infection in dogs in Tehran (Iran). Arch Razi Inst 2018;73:257-263.
  14. Rucksaken R, Maneeruttanarungroj C, Maswanna T, Sussadee M, Kanbutra P. Comparison of conventional polymerase chain reaction and routine blood smear for the detection of Babesia canis, Hepatozoon canis, Ehrlichia canis, and Anaplasma platys in Buriram Province, Thailand. Vet World 2019;12:700-705.
  15. Aktas M, Ozubek S, Altay K, Balkaya I, Utuk AE, Kirbas A, Simsek S, Dumanli N. A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey. Vet Parasitol 2015;209:264-267.
  16. Mundim AV, De Morais IA, Tavares M, Cury MC, Mundim MJS. Clinical and hematological signs associated with dogs naturally infected by Hepatozoon sp. and with other hematozoa: a retrospective study in Uberlândia, Minas Gerais, Brazil. Vet Parasitol 2008;153:3-8.
  17. Bhusri B, Lekcharoen P, Changbunjong T. First detection and molecular identification of Babesia gibsoni and Hepatozoon canis in an Asiatic wild dog (Cuon alpinus) from Thailand. Int J Parasitol Parasites Wildl 2022;17:225-229.
  18. Karagenc TI, Pasa S, Kirli G, Hosgor M, Bilgic HB, Ozon YH, Atasoy A, Eren H. A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey. Vet Parasitol 2006;135:113-119.
  19. Li Y, Wang C, Allen KE, Little SE, Ahluwalia SK, Gao D, Macintire DK, Blagburn BL, Kaltenboeck B. Diagnosis of canine Hepatozoon spp. infection by quantitative PCR. Vet Parasitol 2008;157:50-58.
  20. Sasanelli M, Paradies P, Lubas G, Otranto D, De Caprariis D. Atypical clinical presentation of coinfection with Ehrlichia, Babesia and Hepatozoon species in a dog. Vet Rec 2009; 164:22-23.
  21. Mylonakis ME, Koutinas AF, Baneth G, Polizopoulou Z, Fytianou A. Mixed Ehrlichia canis, Hepatozoon canis, and presumptive Anaplasma phagocytophilum infection in a dog. Vet Clin Pathol 2004;33:249-251.
  22. Baneth G, Weigler B. Retrospective case‐control study of hepatozoonosis in dogs in Israel. J Vet Intern Med 1997;11:365-370.
  23. Vojta L, Mrljak V, Curkovic S, Zivicnjak T, Marinculic A. Molecular epizootiology of canine hepatozoonosis in Croatia. Int J Parasitol 2009;39:1129-1136.
  24. Baneth G. Perspectives on canine and feline hepatozoonosis. Vet Parasitol 2011;181:3-11.
  25. Assarasakorn S, Niwetpathomwat A, Techangamsuwan S, Suvarnavibhaja S. A retrospective study of clinical hematology and biochemistry of canine hepatozoonosis on hospital populations in Bangkok, Thailand. Comp Clin Pathol 2006;15:107-109.
  26. Ybañez AP, Ybañez RH, Villavelez RR, Malingin HPF, Barrameda DNM, Naquila SV, Olimpos SMB. Retrospective analyses of dogs found serologically positive for Ehrlichia canis in Cebu, 1 Philippines from 2003 to 2014. Vet World 2016;9:43-47.
  27. Aubert S, Crosa PA, Serrano D, Esteban Rossanigo C. Canine Hepatozoonosis: a case in San Luis (Argentina). in Proceedings 23rd International Conference of the World Association for the Advancement of Veterinary Parasitology. Pag. 2011.
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