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فیلم دوره آموزشی هوش مصنوعی: SWOT و سیاست‌های ناشران بین‌المللی
ارتقاء رتبه نشریه علمی «شعرپژوهی» دانشگاه شیراز در ارزیابی سامانه رتبه‌بندی نشریات کشور
افتخارآفرینی دبیران مجلات علمی دانشگاه شیراز در عرصۀ بین‌المللی
اطلاعیه برگزاری دوره آموزشی هوش مصنوعی: SWOT و سیاست‌های ناشران بین‌المللی در تاریخ 19 خرداد 1404
ارتقاء رتبۀ مجلۀ علوم ایران از Q3 به Q2

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Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. (1402). Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. , 12(2), 87-94. doi: 10.22099/mbrc.2023.47151.1821
Ehsan Zoaktafi; Hassan Sharifiyazdi; Nooshin Derakhshandeh; Farnoosh Bakhshaei-shahrbabaki. "Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations". , 12, 2, 1402, 87-94. doi: 10.22099/mbrc.2023.47151.1821
Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. (1402). 'Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations', , 12(2), pp. 87-94. doi: 10.22099/mbrc.2023.47151.1821
Zoaktafi, Ehsan, Sharifiyazdi, Hassan, Derakhshandeh, Nooshin, Bakhshaei-shahrbabaki, Farnoosh. Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. , 1402; 12(2): 87-94. doi: 10.22099/mbrc.2023.47151.1821

Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations

Molecular Biology Research Communications
دوره 12، شماره 2، 2023، صفحه 87-94    اصل مقاله (256.47 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.47151.1821
نویسندگان
Ehsan Zoaktafi؛ Hassan Sharifiyazdi؛ Nooshin Derakhshandeh* ؛ Farnoosh Bakhshaei-shahrbabaki
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده
Canine hepatozoonosis is a tick-transmitted apicomplexan infection caused by two species of Hepatozoon, H. canis, and H. americanum. The present research aimed at detection of Hepatozoon spp. in dogs and its effects on hematological alterations. Blood samples were taken from 108 dogs to assess Hepatozoon spp. Phylogenetic analysis was performed based on the 18S rDNA marker by PCR assay and Giemsa-stained blood smear examination. Of the 108 blood samples of dogs tested in the present study, eight (7.40%, 95% CI: 3.25-14.07%) were positive by the Hepatozoon-specific PCR assay. However, in the microscopic examination, only one sample (0.93%) was positive. All of the sequenced samples were H. canis. The Hepatozoon sequences obtained from PCR amplicons in the canine-positive cases exhibited 100% similarity to each other and 98.47-100% similarity to other relevant sequences in GenBank. These findings represent the first molecular evidence of H. canis in dog populations in South Iran. Furthermore, according to the hematological analysis, significantly higher average numbers of neutrophils and lymphocytes were found in the infected group compared to the non-infected dogs. In this study, no statistically significant connection (P<0.05) was observed between H. canis infection and the examined risk factors. 
کلیدواژه‌ها
Hepatozoon canis؛ Molecular detection؛ Dog؛ Hematological analysis
مراجع
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