اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها929
تعداد مقالات8,346
تعداد مشاهده مقاله16,802,334
تعداد دریافت فایل اصل مقاله14,116,912
Zamani, Bahareh, Ramazani, Amin, Saberzadeh, Jamileh, Rostampour, Puria, Takhshid, Mohammad Ali. (1402). The effect of Annexin A5 overexpression on invasiveness and expression of the genes involved in epithelial-mesenchymal transition of HCT 116 cell line. , 12(2), 77-85. doi: 10.22099/mbrc.2023.47160.1823
Bahareh Zamani; Amin Ramazani; Jamileh Saberzadeh; Puria Rostampour; Mohammad Ali Takhshid. "The effect of Annexin A5 overexpression on invasiveness and expression of the genes involved in epithelial-mesenchymal transition of HCT 116 cell line". , 12, 2, 1402, 77-85. doi: 10.22099/mbrc.2023.47160.1823
Zamani, Bahareh, Ramazani, Amin, Saberzadeh, Jamileh, Rostampour, Puria, Takhshid, Mohammad Ali. (1402). 'The effect of Annexin A5 overexpression on invasiveness and expression of the genes involved in epithelial-mesenchymal transition of HCT 116 cell line', , 12(2), pp. 77-85. doi: 10.22099/mbrc.2023.47160.1823
Zamani, Bahareh, Ramazani, Amin, Saberzadeh, Jamileh, Rostampour, Puria, Takhshid, Mohammad Ali. The effect of Annexin A5 overexpression on invasiveness and expression of the genes involved in epithelial-mesenchymal transition of HCT 116 cell line. , 1402; 12(2): 77-85. doi: 10.22099/mbrc.2023.47160.1823

The effect of Annexin A5 overexpression on invasiveness and expression of the genes involved in epithelial-mesenchymal transition of HCT 116 cell line

Molecular Biology Research Communications
دوره 12، شماره 2، 2023، صفحه 77-85    اصل مقاله (268.38 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.47160.1823
نویسندگان
Bahareh Zamani1؛ Amin Ramazani2؛ Jamileh Saberzadeh1؛ Puria Rostampour1؛ Mohammad Ali Takhshid* 1
1Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
چکیده
Epithelial-to-mesenchymal transition (EMT) plays a critical role in colorectal cancer (CRC) metastasis. In the present study, we evaluated the effects of annexin A5 (ANXA5) overexpression on invasiveness as well as the expression of genes involved in EMT of HCT 116 cell line. PCMV6-AC-IRES-GFP plasmid harboring ANXA5 cDNA was constructed. HCT 116 cell line was transfected with recombinant plasmids using Lipofectamine 3000. Fluorescent microscopy was used to determine the efficiency of plasmid transfection. Cell viability was determined using the MTT assay. HCT 116 cell migration was evaluated using wound healing assay and transwell migration assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression of genes involved in EMT. The results of RT-qPCR showed overexpression of ANXA5 compared to the control group. ANXA5 overexpression had no significant effects on cell viability but significantly decreased the rate of wound closure in the wound healing assay as well as the number of migrated cells in transwell assay. Furthermore, ANXA5 overexpression decreased the expression of N-cadherin, Snail, Slug, MMP-2, and MMP-9 while the expression of E-cadherin increased following ANXA5 overexpression. However, VEGF expression did not significantly change after ANXA5 overexpression. Results of the present study suggest that ANXA5 overexpression might have inhibitory effects on the metastasis of CRC through modulating the expression of EMT- related genes.
کلیدواژه‌ها
Colorectal cancer؛ Epithelial to mesenchymal transition؛ Metastasis؛ ANXA5
مراجع
  1. O'Sullivan DE, Sutherland RL, Town S, Chow K, Fan J, Forbes N, Heitman SJ, Hilsden RJ, Brenner DR. Risk factors for early-onset colorectal cancer: A systemic review and meta-analysis. Clin Gastroenterol Hepatol. 2022;20:1229-1240.e5.
  2. Vu T, Datta PK. Regulation of EMT in colorectal cancer: a culprit in metastasis. Cancers (Basel) 2017;9:171.
  3. Balch C, Dedman JR. Annexins II and V inhibit cell migration. Exp Cell Res 1997;237:259-263.
  4. Ahmadiankia N, Khosravi A. Significance of epithelial-to-mesenchymal transition inducing transcription factors in predicting distance metastasis and survival in patients with colorectal cancer: A systematic review and meta-analysis. J Res Med Sci 2020;25:60.
  5. Boesch M, Spizzo G, Seeber A. Concise review: Aggressive colorectal cancer: Role of epithelial cell adhesion molecule in cancer stem cells and epithelial‐to‐mesenchymal transition. Stem Cells Transl Med 2018;7:495-501.
  6. Peng B, Guo C, Guan H, Liu S, Sun MZ. Annexin A5 as a potential marker in tumors. Clin Chim Acta 2014;427:42-48.
  7. van Genderen HO, Kenis H, Hofstra L, Narula J, Reutelingsperger CP. Extracellular annexin A5: functions of phosphatidylserine-binding and two-dimensional crystallization. Biochim Biophys Acta 2008;1783:953-963.
  8. Gerke V, Moss SE. Annexins: from structure to function. Physiol Rev 2002;82:331-371.
  9. Sun X, Liu S, Wang J, Wei B, Guo C, Chen C, Sun MZ. Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. Cell Death Dis 2018;9:1-16.
  10. Ji C, Guo H, Zhang P, Kuang W, Fan Y, Wu L. AnnexinA5 promote glioma cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway. J Neurooncol 2018;138:469-478.
  11. Li L, Zou J, Dai Y, Fan W, Niu G, Yang Z, Chen X. Burst release of encapsulated annexin A5 in tumours boosts cytotoxic T-cell responses by blocking the phagocytosis of apoptotic cells. Nat Biomed Eng 2020;4:1102-1116.
  12. Wang X, Dai Y, Zhao Y, Li M, Zhang J, Ci Y, Wang H, Li X. AnnexinA5 might suppress the phenotype of human gastric cancer cells via ERK pathway. Front Oncol 2021;11:1-9.
  13. Wang J, Zhang Y, Liu X, Ma J, Liu P, Hu C, Zhang G. Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncol Rep 2014;32:2557-2563.
  14. Gong L, Zhao H, Wang L, Sun B, Yu L, Qi X, Zhou X, Zhang L, Guan H, Shao S. Upregulation of annexin A5 affects the biological behaviors of lung squamous carcinoma cells in vitro. Chinese Science Bulletin 2014;59:3610-3620.
  15. Sun CB, Zhao AY, Ji S, Han XQ, Sun ZC, Wang MC, Zheng FC. Expression of annexin A5 in serum and tumor tissue of patients with colon cancer and its clinical significance. World J Gastroenterol 2017;23:7168-7173.
  16. Faraji SN, Mojtahedi Z, Ghalamfarsa G, Takhshid MA. N-myc downstream regulated gene 2 overexpression reduces matrix metalloproteinase-2 and-9 activities and cell invasion of A549 lung cancer cell line in vitro. Iran J Basic Med Sci 2015;18:773-779.
  17. Golestan A, Mojtahedi Z, Ghalamfarsa G, Hamidinia M, Takhshid MA. The effects of NDRG2 overexpression on cell proliferation and invasiveness of SW48 colorectal cancer cell line. Iran J Med Sci 2015;40:430-439.
  18. Monfared MM, Zarei MA, Dehbidi GR, Behbahani AB, Arabsolghar R, Takhshid MA. NDRG2 regulates the expression of genes involved in epithelial mesenchymal transition of prostate cancer cells. Iran J Med Sci 2019;44:118-126.
  19. Li X, Ma W, Wang X, Ci Y, Zhao Y. Annexin A5 overexpression might suppress proliferation and metastasis of human uterine cervical carcinoma cells. Cancer Biomark 2018;23:23-32.
  20. Wehder L, Arndt S, Murzik U, Bosserhoff AK, Kob R, von Eggeling F, Melle C. Annexin A5 is involved in migration and invasion of oral carcinoma. Cell Cycle 2009;8:1552-1558.
  21. Sun X, Wei B, Liu S, Guo C, Wu N, Liu Q, Sun MZ. Anxa5 mediates the in vitro malignant behaviours of murine hepatocarcinoma Hca-F cells with high lymph node metastasis potential preferentially via ERK2/p-ERK2/c-Jun/pc-Jun (Ser73) and E-cadherin. Biomed Pharmacother 2016;84:645-654.
  22. Nakao H, Watanabe M, Maki M. A new function of calphobindin I (annexin V) Promotion of both migration and urokinase‐type plasminogen activator activity of normal human keratinocytes. Eur J Biochem 1994;223:901-908.
  23. Watanabe M, Kondo S, Mizuno K, Yano W, Nakao H, Hattori Y, Kimura K, Nishida T. Promotion of corneal epithelial wound healing in vitro and in vivo by annexin A5. Invest Ophthalmol Vis Sci 2006;47:1862-1888.
  24. Kaszak I, Witkowska-Piłaszewicz O, Niewiadomska Z, Dworecka-Kaszak B, Ngosa Toka F, Jurka P. Role of cadherins in cancer - a review. Int J Mol Sci 2020;21:7624.
  25. Serrano-Gomez SJ, Maziveyi M, Alahari SK. Regulation of epithelial-mesenchymal transition through epigenetic and post-translational modifications. Mol Cancer 2016;15:1-14.
  26. Fan F, Samuel S, Evans KW, Lu J, Xia L, Zhou Y, Sceusi E, Tozzi F, Ye XC, Mani SA, Ellis LM. Overexpression of Snail induces epithelial–mesenchymal transition and a cancer stem cell–like phenotype in human colorectal cancer cells. Cancer Med 2012;1:5-16.
  27. Barabás L, Hritz I, István G, Tulassay Z, Herszényi L. The behavior of MMP-2, MMP-7, MMP-9, and their inhibitors TIMP-1 and TIMP-2 in adenoma-colorectal cancer sequence. Dig Dis 2021;39:217-224.
  28. Kostova E, Slaninka-Miceska M, Labacevski N, Jakovski K, Trojachanec J, Atanasovska E, Jovanovik R, Janevska V. Expression of matrix metalloproteinases 2, 7 and 9 in patients with colorectal cancer. Vojnosanit Pregl 2014;71:52-59.
  29. Mook OR, Frederiks WM, Van Noorden CJ. The role of gelatinases in colorectal cancer progression and metastasis. Biochim Biophys Acta 2004;1705:69-89.
آمار
تعداد مشاهده مقاله: 2,035
تعداد دریافت فایل اصل مقاله: 1,189


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب