| تعداد نشریات | 24 |
| تعداد شمارهها | 862 |
| تعداد مقالات | 7,675 |
| تعداد مشاهده مقاله | 13,669,375 |
| تعداد دریافت فایل اصل مقاله | 11,743,868 |
Comparison of biofilm production and virulence genes distribution among human and canine isolates of Staphylococcus aureus | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 1 - شماره پیاپی 82، 2023، صفحه 74-80 اصل مقاله (449.65 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.43373.6331 | ||
| نویسندگان | ||
| A. Šmitran* 1؛ Ž. Sladojević2؛ L. Božić1؛ I. Gajić3؛ T. Marković1؛ D. Kasagić2؛ I. Subić4؛ G. Katalina5؛ B. Golić6 | ||
| 1Department of Microbiology and Immunology, Faculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and Herzegovina | ||
| 2Laboratory for Molecular Microbiology, PI Veterinary Institute of the Rublic Srpska “Dr Vaso Butozan”, Branka Radičevića 18, 78000 Banja Luka, Bosnia and Herzegovina | ||
| 3Department of Bacteriology, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotića 1, 11000 Belgrade, Serbia | ||
| 4MSc in Molecular Microbiology, Laboratory for Molecular Microbiology, PI Veterinary Institute of the Rublic Srpska “Dr Vaso Butozan”, Branka Radičevića 18, 78000 Banja Luka, Bosnia and Herzegovina | ||
| 5MSc in Medical Care, Department of Nursing, Faculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and Herzegovina | ||
| 6Laboratory for Microbiology of Food, Feed and Water, PI Veterinary Institute of the Rublic Srpska “Dr Vaso Butozan”, Branka Radičevića 18, 78000 Banja Luka, Bosnia and Herzegovina | ||
| چکیده | ||
| Background: Staphylococcus aureus is an important human and animal pathogen that can cause a wide range of infections due to numerous virulence factors. Aims: The aim of this study was to compare biofilm formation ability with different virulence factors such as bacterial motility, genes encoding biofilm associated proteins, and Panton-Valentine leukocidin (PVL) among human and canine isolates of S. aureus. Methods: A total of 60 human (30 methicillin sensitive S. aureus (MSSA) and 30 methicillin resistant S. aureus (MRSA)) and 17 canine (all MSSA) isolates of S. aureus were tested for the capability of biofilm production, motility assay, and presence of genes encoding virulence factors: ica (encoding intercellular adhesion), bap (encoding biofilm-associated protein), fnbA (encoding fibronectin-binding protein A), cna (encoding collagen-binding protein), and pvl (encoding PVL). Results: Animal isolates of S. aureus performed better biofilm production than the human strains (P=0.042), as well as human MSSA compared to the MRSA isolates (P=0.013). Our results showed that cna, fnbA, and ica genes (67.5%, 66.2%, and 42.9%, respectively) were more prevalent than bap and pvl genes (0%, and 7.8%, respectively). The ica gene was significantly more prevalent in human isolates compared to animal isolates (n=31/60 vs. n=2/17, P=0.008), whereas the cna gene was more frequent in animal isolates than in human ones (n=15/17 vs. n=37/60, P=0.0201). Significant correlations were found between the biofilm formation of animal isolates, and the presence of fnbA (P=0.029) and ica genes (P=0.001). Conclusion: This study showed a correlation between biofilm production and the presence of certain biofilm-related genes in animal isolates, as well as stronger biofilm production among MSSA human and animal isolates. | ||
| کلیدواژهها | ||
| Biofilm؛ Biofilm-associated genes؛ pvl gene؛ Staphylococcus aureus | ||
| مراجع | ||
|
Asanin, J; Misic, D; Aksentijevic, K; Tambur, Z; Rakonjac, B; Kovacevic, I; Spergser, J and Loncaric, I (2019). Genetic profiling and comparison of human and animal methicillin-resistant Staphylococcus aureus (MRSA) isolates from Serbia. Antibiotics (Basel). 8: 26.
Bakthavatchalam, YD; Nabarro, LEB; Ralph, R and Veeraraghavan, B (2017). Diagnosis and management of Panton-Valentine leukocidin toxin associated Staphylo-coccus aureus infection: an update. Virulence. 8: 1036.
Bissong, MEA and Ateba, CN (2020). Genotypic and phenotypic evaluation of biofilm production and antimicrobial resistance in Staphylococcus aureus isolated from milk, North West Province, South Africa. Antibiotics. 9: 156.
Budimir, A; Payerl Pal, M; Bošnjak, Z; Mareković, I; Vuković, D; Roksandić Križan, I; Milas, J; Plečko, V and Kalenić, S (2014). Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus strains isolated in a multicenter study of nursing home residents in Croatia. Am. J. Infect. Control. 42: 1197-1202.
Castellazzi, ML; Bosis, S; Borzani, I; Tagliabue, C; Pinzani, R; Marchisio, P and di Pietro, GM (2021). Panton-Valentine leukocidin Staphylococcus aureus severe infection in an infant: a case report and a review of the literature. Ital. J. Pediatr., 47: 158.
Chen, L; Tang, ZY; Cui, SY; Ma, ZB; Deng, H; Kong, WL; Yang, LW; Lin, C; Xiong, WG and Zeng, ZL (2020). Biofilm production ability, virulence and antimicrobial resistance genes in Staphylococcus aureus from various veterinary hospitals. Pathogens. 9: 264.
Conrad, JC (2012). Physics of bacterial near-surface motility using flagella and type IV pili: implications for biofilm formation. Res. Microbiol., 163: 619-629.
Cucarella, C; Solano, C; Valle, J; Amorena, B; Lasa, I and Penadés, JR (2001). Bap, a Staphylococcus aureus surface protein involved in biofilm formation. J. Bacteriol., 183: 2888-2896.
Cucarella, C; Tormo, MA; Ubeda, C; Trotonda, MP; Monzon, M; Peris, C; Amorena, B; Lasa, I and Penadés, JR (2004). Role of biofilm-associated protein bap in the pathogenesis of bovine Staphylococcus aureus. Infect. Immun., 72: 2177-2185.
Donlan, RM and Costerton, JW (2002). Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev., 215: 167-193.
Edwards, AM; Bowden, MG; Brown, EL; Laabei, M and Massey, RC (2012). Staphylococcus aureus extracellular adherence protein triggers TNFalpha release, promoting attachment to endothelial cells via protein a. PLoS One. 7: e43046.
El-Nagdy, AH; Abdel-Fattah, GM and Emarah, Z (2020). Detection and control of biofilm formation by Staphylococcus aureus from febrile neutropenic patient. Infect. Drug. Resist., 13: 3091-3101.
Ghasemian, A; Najar Peerayeh, S; Bakhshi, B and Mirzaee, M (2015). The microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) genes among clinical isolates of Staphylococcus aureus from hospitalized children. Iran. J. Pathol., 10: 258-264.
Hanitsch, LG; Krüger, R; Hoppe, PA; Humme, D; Pokrywka, A; Niebank, M; Stegemann, M; Kola, A and Leistner, R (2020). Outpatient decolonization after recurrent skin infection with Panton-Valentine leukocidin (PVL) producing S. aureus-the importance of treatment repetition. PLoS One. 15: e0231772.
Isenberg, HD (2004). Clinical microbiology procedures handbook. 2nd Edn., Washington D.C., USA, ASM Press. PP: 110-122.
Kaito, C and Sekimizu, K (2007). Colony spreading in Staphylococcus aureus. J. Bacteriol., 189: 2553-2557.
Khoramian, B; Jabalameli, F; Niasari-Naslaji, A; Taherikalani, M and Emaneini, M (2015). Comparison of virulence factors and biofilm formation among Staphylococcus aureus strains isolated from human and bovine infections. Microb. Pathog., 88: 73-77.
Kıvanç, SA; Arık, G; Akova-Budak, B and Kıvanç, M (2018). Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/ icaD/bap genotype isolated from ocular surface of patients with diabetes. Malawi. Med. J., 30: 243-249.
Ko, DS; Kim, NH; Kim, EK; Ha, EJ; Ro, YH; Kim, D; Choi, KS and Kwon, HJ (2021). Comparative genomics of bovine mastitis-origin Staphylococcus aureus strains classified into prevalent human genotypes. Res. Vet. Sci., 139: 67-77.
Li, L; Yang, HJ; Liu, DC; He, HB; Wang, CF; Zhang, JF; Gao, YD and Yangjun, Z (2012). Analysis of biofilm formation and associated genes detected in Staphylococcus isolates from bovine mastitis. Intern. J. Appl. Res. Vet. Med., 10: 62-68.
Lina, G; Piémont, Y; Godail-Gamot, F; Bes, M; Peter, MO; Gauduchon, V; Vandenesch, F and Etienne, J (1999). Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis., 29: 1128-1132.
Lisowska-Łysiak, K; Kosecka-Strojek, M; Białecka, J; Kasprowicz, A; Garbacz, K; Piechowicz, L; Kmet, V; Savini, V and Międzobrodzki, J (2019). New insight into genotypic and phenotypic relatedness of Staphylococcus aureus strains from human infections or animal reservoirs. Pol. J. Microbiol., 68: 93-104.
Monistero, V; Graber, HU; Pollera, C; Cremonesi, P; Castiglioni, B; Bottini, E; Ceballos-Marquez, A; Lasso-Rojas, L; Kroemker, V; Wente, N; Petzer, IM; Santisteban, C; Runyan, J; Veiga Dos Santos, M; Alves, BG; Piccinini, R; Bronzo, V; Abbassi, MS; Said, MB and Moroni, P (2018). Staphylococcus aureus isolates from bovine mastitis in eight countries: genotypes, detection of genes encoding different toxins and other virulence genes. Toxins (Basel). 10: 247. Namvar, AE; Asghari, B; Ezzatifar, F; Azizi, G and Lari, AR (2013). Detection of the intercellular adhesion gene cluster (ica) in clinical Staphylococcus aureus isolates. GMS. Hyg. Infect. Control. 8: Doc03.
Oh, JY; Chae, JC; Han, JI; Song, WK; Lee, CM and Park, HM (2020). Distribution and epidemiological relatedness of methicillin-resistant Staphylococcus aureus isolated from companion dogs, owners, and environments. J. Vet. Med. Sci., 82: 1379-1386.
Otton, LM; da Silva Campos, M; Meneghetti, KL and Corção, G (2017). Influence of twitching and swarming motilities on biofilm formation in Pseudomonas strains. Arch. Microbiol., 199: 677-682.
Pereyra, EA; Picech, F; Renna, MS; Baravalle, C; Andreotti, CS; Russi, R; Calvinho, LF; Diez, C and Dallard, BE (2016). Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells. Vet. Microbiol., 183: 69-77.
Peton, V and Le Loir, Y (2014). Staphylococcus aureus in veterinary medicine. Infect. Genet. Evol., 21: 602-615.
Pollitt, EJ; Crusz, SA and Diggle, SP (2015). Staphylococcus aureus forms spreading dendrites that have characteristics of active motility. Sci. Rep., 5: 17698.
Qi, L; Li, H; Zhang, C; Liang, B; Li, J; Wang, L; Du, X; Liu, X; Qiu, S and Song, H (2016). Relationship between antibiotic resistance, biofilm formation, and biofilm-specific resistance in Acinetobacter baumannii. Front. Microbiol., 7: 483.
Rebić, V; Budimir, A; Aljicević, M; Mahmutović Vranić, S and Rebić, D (2019). Panton-Valentine leukocidin and staphylococcal cassette chromosome mec characterization of community acquired methicillin-resistant Staphylo-coccus aureus. Cent. Eur. J. Public. Health. 27: 58-63.
Stepanovic, S; Vukovic, D; Dakic, I; Savic, B and Svabic-Vlahovic, M (2000). A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J. Microbiol. Methods., 40: 175-179.
Tang, J; Chen, J; Li, H; Zeng, P and Li, J (2013). Characterization of adhesin genes, staphylococcal nuclease, hemolysis, and biofilm formation among Staphylococcus aureus strains isolated from different sources. Foodborne. Pathog. Dis., 10: 757-763.
Tangchaisuriya, U; Yotpanya, W; Kitti, T and Sitthisak, S (2014). Distribution among Thai children of methicillin-resistant Staphylococcus aureus lacking cna, fnbA and icaAD. Southeast Asian J. Trop. Med. Public Health. 45: 149-156.
The European Committee on Antimicrobial Susceptibility Testing (2021). Breakpoint tables for interpretation of MICs and zone diameters. Version 11, Accessed 11 Oct. 2021. Available at:https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.xlsx.
Torres, G; Vargas, K; Cuesta-Astroz, Y; Reyes-Vélez, J and Olivera-Angel, M (2020). Phenotypic characterization and whole genome analysis of a strong biofilm-forming Staphylococcus aureus strain associated with subclinical bovine mastitis in Colombia. Front. Vet. Sci., 7: 530.
Yousefi, F; Mousavi, SF; Siadat, SD; Aslani, MM; Amani, J; Rad, HS and Fooladi, AA (2016). Preparation and in vitro evaluation of antitumor activity of TGFαL3-SEB as a ligand-targeted superantigen. Technol. Cancer Res. Treat., 15: 215-226. | ||
|
آمار تعداد مشاهده مقاله: 647 تعداد دریافت فایل اصل مقاله: 459 |
||