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Detection of antimicrobial resistance genes in extended spectrum beta-lactamase-producing Escherichia coli from milk of indigenous Beetal goats of Punjab | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 1 - شماره پیاپی 82، 2023، صفحه 37-41 اصل مقاله (417.73 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.43480.6365 | ||
| نویسندگان | ||
| M. M. Satpathy1؛ N. S. Sharma2؛ P. Kaur* 2؛ A. K. Arora2 | ||
| 1MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| 2Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| چکیده | ||
| Background: Antimicrobial resistance (AMR) is a burning issue in the present era. Mastitis in dairy animals is one of the most important causes of huge production loss to dairy farmers. Aims: The study aims to find the prevalence, antimicrobial resistance profile, and resistance genes in the extended-spectrum beta-lactamase-producing Escherichia coli in mastitic milk. Methods: A total of 125 milk samples were collected from Beetal goats suffering from clinical mastitis from different districts of Punjab and processed for bacterial isolation and further identification. The drug resistance profile of ESBL-producing E. coli and its associations with molecular markers was analyzed using statistical analysis. Results: The prevalence of ESBL-producing E. coli in dairy goats of Punjab was recorded as 6.4%. The isolates showed the highest resistance to the beta-lactam group of antibiotics. The resistance percentages of streptomycin, gentamicin, tetracycline, chloramphenicol, clotrimazole, and colistin were 50%, 37.5%, 50%, 25%, 25%, and 50%, respectively. The isolates showed intermediate resistance to imipenem (12.5%) and tetracycline (25%). The ESBL-producing E. coli isolates harbored the resistance genes blaCTXM (100%), blaTEM (62.5%), blaSHV (25%), blaOXA (37.5%), tetA (37.5%), tetB (25%), aadA (37.5%), sul1 (25%), MOXM (12.5%), DHAM (25%), and blaCMY-2 (50%). Tetracycline and sulphonamide resistances were statistically associated with their respective resistance genes (P<0.05). Streptomycin resistance was not statistically associated with the presence of the aadA gene (P>0.05). The genes blaIMP and blaNDM were not recorded in any of the isolates. In this study, 12.5% of the isolates showed co-resistance to colistin and carbapenem. Conclusion: Antimicrobial resistance is a hot topic and requires immediate attention. | ||
| کلیدواژهها | ||
| Antimicrobial resistance؛ ESBL؛ Mastitis؛ Pathogens | ||
| مراجع | ||
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Barwa, R and Shaaban, M (2017). Molecular characterization of Klebsiella pneumoniae clinical isolates with elevated resistance to carbapenems. Open Microbiol. J., 11: 152-159.
Chen, S; Zhao, S; White, DG; Schroeder, CM; Lu, R; Yang, H; McDermott, PF; Ayers, S and Meng, J (2004). Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from retail meats. Appl. Environ. Microbiol., 70: 1-7.
Coates, AR; Halls, G and Hu, Y (2011). Novel classes of antibiotics or more of the same? Br. J. Pharmacol., 16: 184-194.
Doi, Y; Adams, J; O’Keefe, A; Quereshi, Z; Ewan, L and Paterson, DL (2007). Community-acquired extended-spectrum b-lactamase producers, United States [letter] Emerg. Infect. Dis., [serial on the Internet] Available from: http://www.cdc.gov/EID/content/13/7/1121.htm.
Enne, VI; Cassar, C; Sprigings, K; Woodward, MJ and Bennett, PM (2008). A high prevalence of antimicrobial resistant Escherichia coli isolated from pigs and a low prevalence of antimicrobial resistant E. coli from cattle and sheep in Great Britain at slaughter. FEMS Microbiol. Lett., 278: 193-199.
Gousia, FP; Economou, V; Sakkas, H; Leveidiotou, S and Papadopoulou, C (2011). Antimicrobial resistance of major foodborne pathogens from major meat products. Foodborne Pathog. Dis., 8: 27-38.
Harbottle, H; Thakur, S; Zhao, S and White, DG (2006). Genetics of antimicrobial resistance. Anim. Biotechnol., 17: 111-124.
Maynard, C; Bekal, S; Sanschagrin, F; Levesque, RC; Brousseau, R; Masson, L; Larivière, S and Harel, J (2004). Heterogeneity among virulence and antimicrobial resistance gene profiles of extraintestinal Escherichia coli isolates of animal and human origin. J. Clin. Microbiol., 42: 5444-5452.
Momtaz, H; Rahimi, E and Moshkelani, S (2012). Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran. Vet. Med., 57: 193-197.
Mutua, F; Sharma, G; Grace, D; Bandyopadhyay, S; Shome, B and Lindahl, J (2020). A review of animal health and drug use practices in India, and their possible link to antimicrobial resistance. Antimicrob. Resist. Infect. Control. 9: 103-115.
M’Zali, FH; Heritage, J; Gascoyne-Binzi, DM; Denton, M; Todd, NJ and Hawkey, PM (1997). Transcontinental importation into the UK of Escherichia coli expressing a plasmid-mediated AmpC-type beta-lactamase exposed during an outbreak of SHV-5 extended-spectrum beta-lactamase in a Leeds hospital. J. Antimicrob. Chemother., 40: 823-831.
Paterson, DL and Bonomo, RA (2005). Extended-spectrum beta-lactamases: a clinical update. Clin. Microbiol. Rev., 18: 657-686.
Patrik, B; Rebeccah, T; Carlton, LG; Richard, RS; Nicol, JHL; Vivan, N; Scott, AM; Robert, F and Marie, A (2005). Antimicrobial resistance and virulence genes of Escherichia coli isolates from swine in Ontario. Appl. Environ. Microbiol., 71: 6753-6761.
Peymani, A; Naserpour-Farivar, T; Zare, E and Azarhoosh, KH (2017). Distribution of blaTEM, blaSHV, and blaCTX-M ngenes among ESBL-producing Pseudomonas aeruginosa isolated from Qazvin and Tehran hospitals. Iran. J. Prevent. Med. Hyg., 58: 155-160.
Riffon, R; Sayasith, K; Khalil, H; Dubreuil, P; Drolet, M and Legace, J (2001). Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J. Clin. Microbiol., 39: 2584-2589.
Shaheen, BW; Nayak, R and Boothe, DM (2013). Emergence of a New Delhi metallo-β-lactamase (NDM-1)-encoding gene in clinical Escherichia coli isolates recovered from companion animals in the United States. Antimicrob. Agents Chemother., 57: 2902-2903.
Sharma, C; Rokana, N; Chandra, M; Singh, B; Gulhane, R and Gill, J (2018). Antimicrobial resistance: Its surveillance, impact, and alternative management strategies in dairy animals. Front. Vet. Sci., 4: 237-263.
Shehata, ME; Gamal, M; Amira, HM and Hesham, MS (2016). Molecular and phenotypic characterization of some antimicrobial resistance genes in Escherichia coli isolated from human and broiler chickens. Int. J. Curr. Microbiol. Appl. Sci., 5: 953-965.
Tong, P; Sun, Y; Ji, X; Du, X; Guo, X; Liu, J; Zhu, L; Zhou, B; Zhou, W; Liu, G and Feng, S (2015). Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in Escherichia coli isolated from chickens. Foodborne Pathog. Dis., 12: 345-352.
Van, TTH; Chin, J; Chapman, T; Tran, LT and Coloe, PJ (2008). Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int. J. Food Microbiol., 124: 217-223.
Vishnuraj, M; Kandeepan, G; Rao, K; Chand, S and Kumbhar, V (2016). Occurrence, public health hazards and detection methods of antibiotic residues in foods of animal origin: A comprehensive review. Cogent Food Agric., 2: 1235458-1235465.
Wu, JJ; Ko, WC; Tsai, SHN and Yan, JJ (2007). Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital Antimicrob. Agents Chemother., 51: 1223-1227.
Yu, Y; Ji, S; Chen, Y; Zhou, W; Wei, Z; Li, L and Ma, Y (2007). Resistance of strains producing extended-spectrum β-lactamases and genotype distribution in China. J. Infect., 54: 53-57. | ||
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