اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها874
تعداد مقالات7,803
تعداد مشاهده مقاله14,220,758
تعداد دریافت فایل اصل مقاله12,165,062
Kamalzadeh, Zahra, Hoveizi, Elham, Fereidoonnezhad, Masood. (1401). Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction. , 12(1), 37-49. doi: 10.22099/mbrc.2023.44705.1781
Zahra Kamalzadeh; Elham Hoveizi; Masood Fereidoonnezhad. "Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction". , 12, 1, 1401, 37-49. doi: 10.22099/mbrc.2023.44705.1781
Kamalzadeh, Zahra, Hoveizi, Elham, Fereidoonnezhad, Masood. (1401). 'Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction', , 12(1), pp. 37-49. doi: 10.22099/mbrc.2023.44705.1781
Kamalzadeh, Zahra, Hoveizi, Elham, Fereidoonnezhad, Masood. Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction. , 1401; 12(1): 37-49. doi: 10.22099/mbrc.2023.44705.1781

Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction

Molecular Biology Research Communications
دوره 12، شماره 1، 2023، صفحه 37-49    اصل مقاله (640.6 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.44705.1781
نویسندگان
Zahra Kamalzadeh1؛ Elham Hoveizi* 1؛ Masood Fereidoonnezhad2
1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Medicinal Chemistry, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Toxicity and autophagy effects of a new complex of platinum II (CPC) were evaluated on HeLa cells cultured on a PCL/gelatin electrospinning scaffold. HeLa cells were treated with CPC on the first, third, and fifth days and the concentration of IC50 was determined. The autophagic and apoptotic effects of CPC were examined by MTT assay, Acridine Orange, Giemsa, DAPI, MDC, real-time PCR, Western blot testing, and molecular docking. The cell viability was obtained on days 1, 3, and 5 as much as 50, 7.28, and 19%, respectively with a concentration of IC50 (100μM) of CPC. The staining results indicated that the treatment of HeLa cells with CPC had antitumor and autophagic effects. Results of RT-PCR showed that the expression of BAX, BAD, P53, and LC3 genes was significantly increased in the sample treated with IC50 concentration compared to the control sample whereas the expression of BCL2, mTOR, and ACT genes in cells was significantly decreased compared to the control group. Also, these results were confirmed by Western blotting. The data indicated the induction of apoptotic death and autophagy in the studied cells. The new compound of CPC has antitumor effects.
کلیدواژه‌ها
Autophagy؛ Cycloplatinated(II) Complex؛ mTOR Pathway؛ Scaffold؛ Tumor Cells
مراجع
  1. Huang R, Rofstad EK. Cancer stem cells (CSCs), cervical CSCs and targeted therapies. Oncotarget 2017;8:35351-35367.
  2. Sak K. Characteristic features of cytotoxic activity of flavonoids on human cervical cancer cells. Asian Pac J Cancer Prev 2014;15:8007-8019.
  3. Nies DH. The biological chemistry of the transition metal "transportome" of Cupriavidus metallidurans. Metallomics 2016;8:481-507.
  4. Berry H, Wang B, Zhang Q. The behavior of magnetic properties in the clusters of 4d transition. Molecules 2018;23:1896.
  5. D'Arcy MS. Cell death: a review of the major forms of apoptosis, necrosis and autophagy. Cell Biol Int 2019;43:582-592.
  6. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007;35:495-516.
  7. Lamark T, Svenning S, Johansen T. Regulation of selective autophagy: the p62/SQSTM1 paradigm. Essays Biochem 2017;61:609-624.
  8. Roca-Agujetas V, de Dios C, Leston L, Mari M, Morales A, Colell A. Recent insights into the mitochondrial role in autophagy and its regulation by oxidative stress. Oxid Med Cell Longev 2019;2019:3809308.
  9. Hoveizi E, Ebrahimi-Barough S, Tavakol S, Nabiuni M. In vitro comparative survey of cell adhesion and proliferation of human induced pluripotent stem cells on surfaces of polymeric electrospun nanofibrous and solution-cast film scaffolds. J Biomed Mater Res A 2015; 103:2952-2958.
  10. Hoveizi E, Ebrahimi-Barough S, Tavakol S, Sanamiri K. In vitro differentiation of human iPS cells into neural like cells on a biomimetic polyurea. Mol Neurobiol 2017;54:601-607.
  11. Hoveizi E, Khodadadi S, Tavakol S, Karima O, Nasiri-Khalili MA. Small molecules differentiate definitive endoderm from human induced pluripotent stem cells on PCL scaffold. Appl Biochem Biotechnol 2014;173:1727-1736.
  12. Shahsavari HR, Hu J, Chamyani S, Sakamaki Y, Aghakhanpour RB, Salmon C, Fereidoonnezhad M, Mojaddami A, Peyvasteh P, Beyzavi H. Fluorinated cycloplatinated(II) complexes bearing bisphosphine ligands as potent anticancer agents. Organometallics 2021; 40:72-82.
  13. Sakamaki Y, Ahmadi Mirsadeghi H, Fereidoonnezhad M, Mirzaei F, Moghimi Dehkordi Z, Chamyani S, Alshami M, Abedanzadeh S, Shahsavari HR, Hassan Beyzavi MH. trans-Platinum(II) thionate complexes: Synthesis, structural characterization, and in vitro biological assessment as potent anticancer agents. Chempluschem 2019;84:1525-1535.
  14. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin 2019;69:7-34.
  15. Cheng Q, Liu Y. Multifunctional platinum-based nanoparticles for biomedical applications. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2017;9.
  16. Fereidoonnezhad M, Niazi M, Shahmohammadi Beni M, Mohammadi S, Faghih Z, Shahsavari HR. Synthesis, biological evaluation, and molecular docking studies on the DNA binding interactions of Platinum(II) rollover complexes containing phosphorus donor ligands. ChemMedChem 2017;12:456-465.
  17. Konovalov B, Zivkovic MD, Milovanovic JZ, Djordjevic DB, Arsenijevic AN, Vasic IR, Janjic GV, Franich A, Manojlovic D, Skrivanj S, Milovanovic M, Djuran M, Rajkovic S. Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure. Dalton Trans 2018; 47:15091-15102.
  18. Abdel Ghani NT, Mansour AM. Novel palladium(II) and platinum(II) complexes with 1H-benzimidazol-2-ylmethyl-N-(4-bromo-phenyl)-amine: structural studies and anticancer activity. Eur J Med Chem 2012;47:399-411.
  19. Wattanathamsan O, Treesuwan S, Sritularak B, Pongrakhananon V. Cypripedin, a phenanthrenequinone from Dendrobium densiflorum, sensitizes non-small cell lung cancer H460 cells to cisplatin-mediated apoptosis. J Nat Med 2018;72:503-513.
  20. Yang Y, Li S, Sun Y, Zhang D, Zhao Z, Liu L. Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2. Onco Targets Ther 2019;12:897-906.
  21. Ouyang L, Shi Z, Zhao S, Wang FT, Zhou TT, Liu B, Bao JK. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif 2012;45:487-498.
  22. Frezza M, Dou QP, Xiao Y, Samouei H, Rashidi M, Samari F, Hemmateenejad B. In vitro and in vivo antitumor activities and DNA binding mode of five coordinated cyclometalated organoplatinum(II) complexes containing biphosphine ligands. J Med Chem 2011;54:6166-6176.
  23. Florea AM, Busselberg D. Cisplatin as an anti-tumor drug: cellular mechanisms of activity, drug resistance and induced side effects. Cancers (Basel) 2011;3:1351-1371.
  24. Guo WJ, Zhang YM, Zhang L, Huang B, Tao FF, Chen W, Guo ZJ, Xu Q, Sun Y. Novel monofunctional platinum (II) complex Mono-Pt induces apoptosis-independent autophagic cell death in human ovarian carcinoma cells, distinct from cisplatin. Autophagy 2013;9:996-1008.
  25. Imamura Y, Mukohara T, Shimono Y, Funakoshi Y, Chayahara N, Toyoda M, Kiyota N, Takao S, Kono S, Nakatsura T, Minami H. Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer. Oncol Rep 2015;33:1837-1843.
آمار
تعداد مشاهده مقاله: 877
تعداد دریافت فایل اصل مقاله: 563


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب