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Seifi, Razieh, Ayat, Hoda, Ahadi, Ali. (1401). Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion. , 12(1), 27-36. doi: 10.22099/mbrc.2023.46450.1804
Razieh Seifi; Hoda Ayat; Ali Mohammad Ahadi. "Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion". , 12, 1, 1401, 27-36. doi: 10.22099/mbrc.2023.46450.1804
Seifi, Razieh, Ayat, Hoda, Ahadi, Ali. (1401). 'Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion', , 12(1), pp. 27-36. doi: 10.22099/mbrc.2023.46450.1804
Seifi, Razieh, Ayat, Hoda, Ahadi, Ali. Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion. , 1401; 12(1): 27-36. doi: 10.22099/mbrc.2023.46450.1804

Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion

Molecular Biology Research Communications
دوره 12، شماره 1، 2023، صفحه 27-36    اصل مقاله (265.34 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.46450.1804
نویسندگان
Razieh Seifi؛ Hoda Ayat* ؛ Ali Mohammad Ahadi
Department of Genetics, Shahrekord University, Rahbar Blvd, P.O Box 115 Shahrekord, Iran
چکیده
Scorpion venom contains various toxin peptides with pharmacological and biological properties. Scorpion toxins specifically interact with membrane ion channels which play key roles in progression of cancer. Therefore, scorpion toxins have received special attention for targeting cancer cells. Two new toxins MeICT and IMe-AGAP, isolated from Iranian yellow scorpion, Mesobuthus eupeus, interact specifically with chloride and sodium channels, respectively. Anti-cancer properties of MeICT and IMe-AGAP have been determined before, in addition they show 81 and 93% similarity with two well-known anti-cancer toxins, CTX and AGAP, respectively. The aim of this study was construction of a fusion peptide MeICT/IMe-AGAP to target different ion channels involved in cancer progression. Design and structure of the fusion peptide were investigated by bioinformatics studies. Two fragments encoding MeICT and IMe-AGAP were fused using overlapping primers by SOEing-PCR. MeICT/IMe-AGAP chimeric fragment was cloned into pET32Rh vector, expressed in Escherichia coli host and analyzed by SDS-PAGE. The in silico studies showed that chimeric peptide with GPSPG linker preserved the three-dimensional structure of both peptides and can be functional. Due to the high expression of chloride and sodium channels in various cancer cells, MeICT/IMe-AGAP fusion peptide can be used as an effective agent to target both channels in cancers, simultaneously.
کلیدواژه‌ها
IMe-AGAP؛ MeICT؛ Anti-cancer peptides؛ Chimeric peptide؛ Scorpion toxins
مراجع
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