| تعداد نشریات | 24 |
| تعداد شمارهها | 917 |
| تعداد مقالات | 8,230 |
| تعداد مشاهده مقاله | 16,275,011 |
| تعداد دریافت فایل اصل مقاله | 13,752,778 |
Identification of coagulase negative staphylococcal species from bovine mastitis in India | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 4 - شماره پیاپی 81، 2022، صفحه 358-362 اصل مقاله (369.49 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.43698.6406 | ||
| نویسندگان | ||
| R. N. Bhavana1؛ R. K. Chaitanya* 2 | ||
| 1MVSc in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, India | ||
| 2Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, India | ||
| چکیده | ||
| Background: Staphylococcal mastitis is a major cause of concern to the dairy industry in India and several countries worldwide. Though Staphylococcus aureus is the major cause, coagulase negative staphylococcal species (CoNS) are being increasingly reported in recent years. Aims: To investigate the incidence of coagulase negative staphylococcal species in bovine mastitis. Methods: Isolation of staphylococci was carried out from 237 milk samples of cows and She buffaloes with clinical and subclinical mastitis from different regions of Andhra Pradesh and Karnataka. CoNS isolates were identified by tube coagulase test using fresh rabbit plasma and coagulase gene PCR. We employed the biochemical test scheme published elsewhere previously for identification of the CoNS isolates up to species and subspecies levels. Seven representative isolates were identified by 16S rDNA sequencing to check the accuracy of biochemical test based identification. Results: The CoNS constitute the majority of the staphylococcal isolates from mastitis (80/125, 64%) in this region. Using biochemical test scheme, the CoNS isolates from bovine mastitis were identified as S. cohnii sub sp. cohnii, S. simulans, S. capitis sub sp. capitis, S. cohnii sub sp. xylosus, and S. lugdunensis. The CoNS species S. schleiferi, S. haemolyticus, S. sciuri, S. xylosus, S. chromogenes, and Macrococcus epidermidis were identified by 16S rDNA sequencing. Conclusion: The 16S rDNA sequencing is the appropriate method for the identification of CoNS species. This study highlighted coagulase negative staphylococcal species as possible etiological agents of mastitis. | ||
| کلیدواژهها | ||
| 16S rDNA sequencing؛ Biochemical test scheme؛ Coagulase negative staphylococci؛ Species identification | ||
| مراجع | ||
|
Akineden, O; Annemuller, C; Hassan, AA; Lammler, C; Wolter, W and Zschock, M (2001). Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. Clin. Diagn. Lab. Immunol., 8: 959-964.
Boerlin, P; Kuhnert, P; Hussy, D and Schaellibaum, M (2003). Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J. Clin. Microbiol., 41: 767-771.
Clarridge, JEIII (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev., 17: 840-862.
De Paulis, AN; Predari, SC; Chazarreta, CD and Santoianni, JE (2003). Five test simple scheme for species level identification of clinically significant coagulase negative staphylococci. J. Clin. Microbiol., 41: 1219-1224. Frey, Y; Rodriguez, JP; Thomann, A; Schwendener, S and Perreten, V (2012). Genetic characterization of anti-microbial resistance in coagulase negative staphylococci from bovine mastitis milk. J. Dairy Sci., 96: 2247-2257.
Iorio, NLP; Ferreira, RBR; Schuenck, RP; Malvar, KL; Brilhante, AP; Nunes, APF; Bastos, CCR and Santos, KRN (2007). Simplified and reliable scheme for species-level identification of Staphylococcus clinical isolates. J. Clin. Microbiol., 45: 2564-2569.
Jey kumar, M; Vinod kumar, G; Bashir, BP and Sudhakar, K (2013). Antibiogram of mastitis pathogens in the milk of crossbred cows in Namakkal district, Tamil Nadu. Vet. World. 6: 354-356.
Kim, J; Hong, J; Lim, JA; Heu, S and Roh, E (2018). Improved multiplex PCR primers for rapid identification of coagulase negative staphylococci. Arch. Microbiol., 200: 73-83.
Markey, BK; Leonard, FC; Archambault, M; Cullinane, A and Maguire, D (2013). Clinical Veterinary Microbiology, second edition, New York, USA, Mosby Elsevier: 29-43.
Persson, Y; Nyman, AKJ and Anderson, GU (2011). Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Vet. Scand., 53: 36-40.
Piessens, V; Vliegher, SD; Verbist, B; Braem, G; Nuffel, AV; Vuyst, L; Heyndrickx, M and Coillie, EV (2012). Characterization of coagulase negative Staphylococcus species from cow milk and environment based on bap, icaA and mecA genes and phenotypic susceptibility to antimicrobials and teat dips. J. Dairy Sci., 95: 7027-7038.
Pyorala, S and Taponen, S (2009). Coagulase negative staphylococci - emerging mastitis pathogens. Vet. Microbiol., 134: 3-8.
Sharma, N; Maiti, SK and Sharma, KK (2007). Prevalence, etiology and antibiogram of microorganisms associated with sub-clinical mastitis in buffaloes in Durg, Chhattisgarh State (India). Int. J. Dairy Sci., 2: 145-151.
Srednik, ME; Tremblay, YDN; Labrie, J; Archambault, M; Jacques, M; Cirelli, AF and Gentilini, ER (2017). Biofilm formation and antimicrobial resistance genes of coagulase negative staphylococci isolated from cows with mastitis in Argentina. FEMS Microbiol., 364: 1-8.
Suresh Sah, S; Priyanka, B; Vijaya, D; Satish Kumar, A; Sheela Devi, C; Indumathi, VA and Prashanth, K (2018). Simple and economical method for identification and speciation of Staphylococcus epidermidis and other coagulase negative staphylococci and its validation by molecular methods. J. Microbiol. Methods. 149: 106-119.
Tremblay, YDN; Lamarche, D; Chever, P; Haine, D; Messier, S and Jacques, M (2013). Characterization of the ability of coagulase negative staphylococci isolated from the milk of Canadian farms to form biofilms. J. Dairy Sci., 96: 234-246.
Trujillo, GUB; Rivera, JLS; Solorzano, IR; German, SIC; Martinez, JAB; Garibay, RIA; Aguirre, VMB; Juarez, MC; Patino, AB and Alarcon, JJV (2013). Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis. J. Med. Microbiol., 62: 369-376.
Wang, W; Lin, X; Jiang, T; Peng, Z; Xu, J; Yi, L; Li, F; Fanning, S and Baloch, Z (2018). Prevalence and characterization of Staphylococcus aureus cultured from raw milk taken from dairy cows with mastitis in Beijing, China. Front. Microbiol., 9: 1123-1129. | ||
|
آمار تعداد مشاهده مقاله: 1,012 تعداد دریافت فایل اصل مقاله: 815 |
||