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Identification of coagulase negative staphylococcal species from bovine mastitis in India | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 4 - شماره پیاپی 81، 2022، صفحه 358-362 اصل مقاله (369.49 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.43698.6406 | ||
| نویسندگان | ||
| R. N. Bhavana1؛ R. K. Chaitanya* 2 | ||
| 1MVSc in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, India | ||
| 2Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, India | ||
| چکیده | ||
| Background: Staphylococcal mastitis is a major cause of concern to the dairy industry in India and several countries worldwide. Though Staphylococcus aureus is the major cause, coagulase negative staphylococcal species (CoNS) are being increasingly reported in recent years. Aims: To investigate the incidence of coagulase negative staphylococcal species in bovine mastitis. Methods: Isolation of staphylococci was carried out from 237 milk samples of cows and She buffaloes with clinical and subclinical mastitis from different regions of Andhra Pradesh and Karnataka. CoNS isolates were identified by tube coagulase test using fresh rabbit plasma and coagulase gene PCR. We employed the biochemical test scheme published elsewhere previously for identification of the CoNS isolates up to species and subspecies levels. Seven representative isolates were identified by 16S rDNA sequencing to check the accuracy of biochemical test based identification. Results: The CoNS constitute the majority of the staphylococcal isolates from mastitis (80/125, 64%) in this region. Using biochemical test scheme, the CoNS isolates from bovine mastitis were identified as S. cohnii sub sp. cohnii, S. simulans, S. capitis sub sp. capitis, S. cohnii sub sp. xylosus, and S. lugdunensis. The CoNS species S. schleiferi, S. haemolyticus, S. sciuri, S. xylosus, S. chromogenes, and Macrococcus epidermidis were identified by 16S rDNA sequencing. Conclusion: The 16S rDNA sequencing is the appropriate method for the identification of CoNS species. This study highlighted coagulase negative staphylococcal species as possible etiological agents of mastitis. | ||
| کلیدواژهها | ||
| 16S rDNA sequencing؛ Biochemical test scheme؛ Coagulase negative staphylococci؛ Species identification | ||
| مراجع | ||
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