| تعداد نشریات | 24 |
| تعداد شمارهها | 935 |
| تعداد مقالات | 8,388 |
| تعداد مشاهده مقاله | 16,988,551 |
| تعداد دریافت فایل اصل مقاله | 14,377,095 |
Sarcocystis cruzi in Egyptian slaughtered cattle (Bos taurus): epidemiology, morphology and molecular description of the findings | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 4 - شماره پیاپی 81، 2022، صفحه 337-348 اصل مقاله (1.31 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.43498.6363 | ||
| نویسندگان | ||
| I. S. Elshahawy* 1؛ E. Mohammed1؛ A. Gomaa2؛ M. Fawaz1 | ||
| 1Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt | ||
| 2BSc in Veterinary Medicine, Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt | ||
| چکیده | ||
| Background: Sarcocystis spp. are one of the most common foodborne tissue cyst-forming coccidia with a public health and veterinary concern. Aims: The existing study aimed to rectify the epidemiological profile of Sarcocystis spp. infection in the cattle carcasses as well as to explore the structure and phylogenetic features of Sarcocystis spp. isolates. Methods: A total of 292 cattle carcasses were checked for the existence of sarcocysts using light microscopy (LM) via muscle squash (MS) and peptic digestion (PD) analysis from January 2020 to December 2020. Individual sarcocysts from different cattle tissues were selected for morphologic characterization and DNA extraction. Each sarcocyst’s 18S rDNA gene was amplified, sequenced, and analyzed. Results: Overall, 92.5% (270/292) of cattle tissue samples contained microscopic thin walled sarcocysts and were exclusively found in esophagus by light microscopy. A statistically insignificant relationship exists between the prevalence of infection and age groups, gender of cattle, and the seasonal dynamics (P>0.05). Sarcocysts ultrastructural features were completely discussed. Sequencing of 18S rDNA Sarcocystis gene confirmed S. cruzi (identity 99-100%), which was the first molecular identification of the current isolate in the study region. Conclusion: The current survey initially provides a brief account of knowledge about the epidemiology of Sarcocystis spp. infecting cattle and it is considered a starting point for the development of health awareness and efficient preventive schemes for this zoonotic protozoan parasite. | ||
| کلیدواژهها | ||
| Bos taurus؛ Molecular characterization؛ Sarcocystis cruzi؛ Sarcocysts morphology؛ 18S rRNA | ||
| مراجع | ||
|
Abdel-Ghaffar, F; Mehlhorn, H; Bashtar, AR; Sakran, T and El-Fayoumi, H (2009). Life cycle of Sarcocystis camelicanis infecting the camel (Camelus dromedarius) and the dog (Canis familiaris) light and electron microscopic study. Parasitol. Res., 106: 189-195. doi: 10.1007/s00436-009-1648-x.
Abdullah, SH (2021). Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq. Vet. World. 14: 468-474. doi: 10.14202/vetworld.2021.468-474.
Ahmed, AM; Elshraway, NT and Youssef, AI (2016). Survey on Sarcocystis in bovine carcasses slaughtered at the municipal abattoir of El-Kharga, Egypt. Vet. World. 9: 1461-1465. doi: 10.14202/vetworld.2016.1461-1465.
Altschul, SF; Gish, W; Miller, W; Myers, EW and Lipman, DJ (1990). Basic local alignment search tool. J. Mol. Biol., 215: 403-410. doi: 10.1016/S0022-2836(05)80360-2.
Aráoz, V; Silveira, C; Gastón, M; Banchero, G; Riet-Correa, F and Giannitti, F (2019). Fatal Sarcocystis cruzi-induced eosinophilic myocarditis in a heifer in Uruguay. J. Vet. Diagn. Invest., 31: 656-660. doi: 10.1177/1040638719856651.
Badawy, AII; Abouzaid, NZ and Ahmed, HA (2012). Sarcocystis hominis and other Sarcocystis species infecting cattle in Sharkia province, Egypt. J. Am. Sci., 8: 271-275.
Bahari, P; Salehi, M; Seyedabadi, M and Mohammadi, A (2014). Molecular identification of macroscopic and microscopic cysts of Sarcocystis in sheep in North Khorasan Province, Iran. Int. J. Mol. Cell Med., 3: 51-56.
Barham, M; Stützer, H; Karanis, P; Latif, BM and Neiss, WF (2005). Seasonal variation in Sarcocystis species infections in goats in northern Iraq. Parasitology. 130: 151-156. doi: 10.1017/s0031182004006134.
Bucca, M; Brianti, E; Giuffrida, A; Ziino, G; Cicciari, S and Panebianco, A (2011). Prevalence and distribution of Sarcocystis spp. cysts in several muscles of cattle slaughtered in Sicily, Southern Italy. Food Control. 22: 105-108. doi: 10.1016/j.foodcont.2010.05.015.
Chen, X; Zuo, Y; Rosenthal, BM; He, Y; Cui, L and Yang, Z (2011). Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings. Vet. Parasitol., 178: 35-39. doi: 10.1016/j.vetpar. 2010.12.026.
Chiesa, F; Muratore, E; Dalmasso, A and Civera, T (2013). A new molecular approach to assess the occurrence of Sarcocystis spp. in cattle and products thereof: preliminary data. Ital. J. Food Saf., 2: 148-151.
Choi, T; Hong, E; Ryu, S; Sim, C; Chae, JS; Kim, HC; Park, J; Choi, KS; Yu, DH; Yoo, JG and Park, BK (2018). Detection and identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by molecular and ultrastructural studies in naturally infected Korean cattle (Bos taurus coreanae) from Daejeon, Korea. Korean J. Parasitol., 56: 121-127. doi: 10.3347/kjp.2018.56.2.121.
Claveria, FG; San-Pedro, LR; Cruz-Flores, MJ; Nagasawa, H; Suzuki, N and De La Peña, C (2001). Ultrastructural studies of Sarcocystis cruzi (Hasselmann, 1926) Wenyon, 1926 infection in cattle (Bos taurus): Philippine cases. Parasite. 8: 251-254. doi: 10.1051/parasite/2001083251.
Dafedar, A; D’Souza, PE and Mamatha, GS (2011). Prevalence and morphological studies on Sarcocystis species infecting cattle in Bangalore. J. Vet. Parasitol., 25: 183-184.
Davoudi, J; Shabestari, AB; Mozahheb, P; Norouzi, R; Maadinia, M and Mahmoudi, R (2017). A study of Sarcocystis infection in mincemeat using digestion method in Ghazvin, Iran. Arch. Hyg. Sci., 6: 178-181.
Domenis, L; Peletto, S; Sacchi, L; Clementi, E; Genchi, M; Felisari, L; Felisari, C; Mo, P; Modesto, P; Zuccon, F; Campanella, C; Maurella, C; Guidetti, C and Acutis, PL (2011). Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitol. Res., 109: 1677-1687. doi: 10.1007/s00436-011-2441-1.
Dubey, JP (2015). Foodborne and waterborne zoonotic sarcocystosis. Food Waterborne Parasitol., 1: 2-11. https://doi.org/10.1016/j.fawpar.2015.09.001.
Dubey, JP; Speer, CA and Lindsay, DS (2000). In vitro cultivation of schizonts of Sarcocystis speeri Dubey and Lindsay, 1999. J. Parasitol., 86: 671-678. doi: 10.1645/ 0022-3395(2000)086.
El-Kady, AM; Hussein, NM and Hassan, AA (2018). First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt. J. Parasit. Dis., 42: 114-121. doi: 10.1007/s12639-017-0974-7.
El-Morsey, A; Abdo, W; Sultan, K; Elhawary, NM and AbouZaid, AA (2019). Ultrastructural and molecular identification of the sarcocysts of Sarcocystis tenella and Sarcocystis arieticanis infecting domestic sheep (Ovis aries) from Egypt. Acta Parasitol., 64: 501-513. doi: 10.2478/s11686-019-00070-8.
Faghiri, E; Davari, A and Nabavi, R (2019). Histo-pathological survey on Sarcocystis species infection in slaughtered cattle of Zabol-Iran. Turkiye Parazitol. Derg., 43: 182-186. doi: 10.4274/tpd.galenos.2019.6480.
Farhang-Pajuh, F; Yakhchali, M and Mardani, K (2014). Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran. Vet. Res. Forum. 5: 181-186.
Fayer, R (2004). Sarcocystis spp. in human infections. Clin. Microbiol. Rev., 17: 894-902. doi: 10.1128/CMR.17.4.894-902.2004.
Ferreira, MST; Vogel, FSF; Sangioni, LA; Cezar, AS; Braunig, P; de Avilla Botton, S; Camillo, G and Portella, LP (2018). Sarcocystis species identification in cattle hearts destined to human consumption in southern Brazil. Vet. Parasitol. Reg. Stud. Reports. 14: 94-98. doi: 10.1016/j.vprsr.2018.09.002.
Gjerde, B (2013). Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome C oxidase subunit I gene. Int. J. Parasitol., 43: 579-591. doi: 10.1016/j.ijpara. 2013.02.004.
Gjerde, B (2016). Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitol. Res., 115: 1473-1492. https://doi. org/10.1007/s00436-015-4881-5.
Hajimohammadi, B; Dehghani, A; Ahmadi, MM; Eslami, G; Oryan, A and Khamesipour, A (2014). Prevalence and species identification of Sarcocystis in raw hamburgers distributed in Yazd, Iran using PCR-RFLP. J. Food Qual. Hazards Control. 1: 15-20.
Hamidinejat, H; Jalali, MHR and Nabavi, L (2010). Survey on Sarcocystis infection in slaughtered cattle in South-West of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. J. Anim. Vet. Adv., 9: 1724-1726. doi: 10.3923/javaa.2010.1724.1726.
Hornok, S; Mester, A; Takacs, N; Baska, F; Majoros, G; Fok, E; Biksi, I; Nemet, Z; Hornyak, A; Janosi, S and Farkas, R (2015). Sarcocystis infection of cattle in Hungary. Parasit. Vectors. 8: 69-75. doi: 10.1186/s13071-015-0685-9.
Hu, JJ; Huang, S; Wen, T; Esch, GW; Liang, Y and Li, HL (2017). Sarcocystis spp. in domestic sheep in Kunming City, China: prevalence, morphology, and molecular characteristics. Parasite. 24: 30-38. doi: 10.1051/parasite/ 2017025.
Januškevičius, V; Januškevičienė, G; Banys, A; Dailidavičienė, J and Aniulienė, A (2018). Biochemical profile of blood in naturally occurring Sarcocystis infection in cattle. Acta Vet. Brno., 87: 205-211. https://doi.org/10. 2754 /avb201887030205.
Kalubowila, DG; Udagama-Randeniya, PV; Perera, NA and Rajapakse, RP (2004). Seroprevalence of Sarcosystis spp. in cattle and buffaloes from the wet and dry zones of Sri Lanka: a preliminary study. J. Vet. Med. B. Infect. Dis. Vet. Public Health. 51: 89-93. doi: 10.1111/j.1439-0450. 2004.00726.x.
Khalifa, RM; El-Nadi, NA; Sayed, FG and Omran, EK (2008). Comparative morphological studies on three Sarcocystis species in Sohag, Egypt. J. Egypt. Soc. Parasitol., 38: 599-608.
Kirillova, V; Prakas, P; Calero-Bernal, R; Gavarāne, I; Fernández-García, JL; Martínez-González, M; Rudaitytė-Lukošienė, E; Martínez-Estéllez, MÁH; Butkauskas, D and Kirjušina, M (2018). Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain. Parasit. Vectors. 11: 173-182. doi: 10.1186/s13071-018-2694-y.
Latif, B; Vellayan, S; Heo, C; Kannan, M; Omar, E; Abdullah, S and Tappe, D (2013). High prevalence of muscular sarcocystosis in cattle and water buffaloes from Selangor, Malaysia. Trop. Biomed., 30: 699-705.
Lucas, JR; Barrios-Arpi, M; José, R; Stephanie, B; Zarria, J; Namiyama, G; Noelia, T and Gonzales-Viera, O (2019). Ultrastructural description of Sarcocystis sp. in cardiac muscle of naturally infected Alpacas (Vicugna pacos). Iran. J. Parasitol., 14: 174-179.
Mac, PA; Ibrahim, TM; Buba, DM; Airiohuodion, PA and Ambu, S (2018). Prevalence of Sarcocystis species in meat of cattle, pigs and birds slaughtered in North Central Nigeria. J. Vet. Sci. Med. Diagn., 7: 5-11. doi: 10.4172/2325-9590.1000269.
Makhija, M (2012). Histological identification of muscular Sarcocystis: a report of two cases. Indian J. Pathol. Microbiol., 55: 552-554. doi: 10.4103/0377-4929.107813.
Mavi, SA; Teimouri, A; Mohebali, M; Sharifi Yazdi, MK; Shojaee, S; Rezaian, M; Salimi, M and Keshavarz, H (2020). Sarcocystis infection in beef and industrial raw beef burgers from butcheries and retail stores: A molecular microscopic study. Heliyon. 6: e04171. doi: 10.1016/j. heliyon.2020.e04171.
Mehlhorn, H and Frenkel, JK (1980). Ultrastructural comparison of cysts and zoites of Toxoplasma gondii, Sarcocystis muris, and Hammondia hammondi in skeletal muscle of mice. J. Parasitol., 66: 59-67.
Mehlhorn, H; Hartley, WJ and Heydorn, AO (1976). A comparative ultrastructural study of the cyst wall of 13 Sarcocystis species. Protistologica. 12: 451-467.
Mirzaei, M and Rezaei, H (2016). A survey on Sarcocystis spp. infection in cattle of Tabriz city, Iran. J. Parasit Dis., 40: 648-651. doi: 10.1007/s12639-014-0551-2.
Motamedi, GR; Dalimi, A; Nouri, A and Aghaeipour, K (2011). Ultrastructural and molecular characterization of Sarcocystis isolated from camel (Camelus dromedarius) in Iran. Parasitol. Res., 108: 949-954. doi: 10.1007/s00436-010-2137-y.
Moré, G; Basso, W; Bacigalupe, D; Venturini, MC and Venturini, L (2008). Diagnosis of Sarcocystis cruzi, Neospora caninum and Toxoplasma gondii infections in cattle. Parasitol Res., 102: 671-675. doi: 10.1007/s00436-007-0810-6.
Moré, G; Pantchev, A; Skuballa, J; Langenmayer, MC; Maksimov, P; Conraths, FJ; Venturini, MC and Schares, G (2014). Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis species in beef on sale for consumers in Germany. Parasitol. Res., 113: 2223-2230. doi: 10.1007/s 00436-014-3877-x.
Mounika, K; Chennuru, S; Ravipati, V; Tumati, SR and Krovvidi, S (2018). Studies on prevalence and histomorphology of Sarcocystis species infecting cattle in Andhra Pradesh, India. J. Parasit. Dis., 42: 77-80. doi: 10.1007/s12639-017-0968-5.
Mousa, MM; Sokkary, MYE and Hegazy, MA (2021). The prevalence of sarcocystis affecting slaughtered cattle and buffalo at Sirs-Elian abattoir in Egypt. Alex. J. Vet. Sci., 69: 49-58. doi: 10.5455/ajvs.125880.
Murata, R; Suzuki, J; Hyuga, A; Shinkai, T and Sadamasu, K (2018). Molecular identification and characterization of Sarcocystis spp. in horsemeat and beef marketed in Japan. Parasite. 25: 27-35. doi: 10.1051/parasite/2018026.
Nahed, H; Ghoneim, WM and Nader, MS (2014). Occurrence of zoonotics sarcosporidiosis in slaughtered cattle and buffaloes in different abattoirs in Egypt. Global Vet., 13: 809-813. doi: 10.5829/idosi.gv.2014.13.05.86211.
Nematollahia, A; Khoshkerdar, A; Helan, JA; Shahbazi, P and Hassanzadeh, P (2015). A study on the rate of infestation to Sarcocystis cysts in supplied raw hamburgers. J. Parasit. Dis., 39: 276-279. https://doi.org/10.1007/ ms12639-013-0339-9.
Nourani, H; Matin, S; Nouri, A and Azizi, H (2010). Prevalence of thin walled Sarcocystis cruzi and thick-walled Sarcocystis hirsuta or Sarcocystis hominis from cattle in Iran. Trop. Anim. Health Prod., 42: 1225-1227. doi: 10.1007/s11250-010-9552-z.
Nourollahi-Fard, SR; Asghari, M and Nouri, F (2009). Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop. Anim. Health Prod., 41: 1633-1636. doi: 10.1007/s11250-009-9358-z.
Nourollahi-Fard, SR; Kheirandish, R and Sattari, S (2015). Prevalence and histopathological finding of thin-walled and thick-walled Sarcocysts in slaughtered cattle of Karaj abattoir, Iran. J. Parasit. Dis., 39: 272-275. https://doi.org/ 10.1007/s12639-013-0341-2.
Okur, H; Kandemir, O and Sahine, I (1995). An investigation on Sarcocystis species in cattle and sheep from Bayburt. Turkiye Parazitol. Derg., 19: 113-118.
Ota, T; Nakano, Y; Mizuno, T; Shiozaki, A; Hori, Y; Yamanishi, K; Hayakawa, K; Hayakawa, T; Fujimoto, T; Nakamoto, C; Maejima, K; Wada, Y; Terasoma, F and Ohnishi, T (2019). First case report of possible Sarcocystis truncata-induced food poisoning in venison. Intern Med., 58: 2727-2730. doi: 10.2169/internalmedicine. 2817-19.
Petrie, A and Watson, P (2013). Statistics for veterinary and animal science. 1st Edn., Oxford, Blackwell Publishing. PP: 101-109.
Portella, LP; Fernandes, F; Rodrigues, FS; Minuzzi, CE; Sangioni, LA; Flores, MM and Vogel, FSF (2021). Macroscopic, histological, and molecular aspects of Sarcocystis spp. infection in tissues of cattle and sheep. Rev. Bras. Parasitol. Vet., 30: e003621. doi: 10.1590/ S1984-29612021050.
Prakas, P; Strazdaitė-Žielienė, Ž; Januškevičius, V; Chiesa, F; Baranauskaitė, A; Rudaitytė-Lukošienė, E; Servienė, E; Petkevičius, S and Butkauskas, D (2020). Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method. Parasit. Vectors. 13: 1-9. https://doi.org/10.1186/s13071-020-04473-9.
Rosenthal, BM (2013). Sarcocystosis: Hunter’s tropical medicine and emerging infectious disease. 9th Edn., Chapter 103, Amsterdam, Netherlands, Elsevier. PP: 780-783. doi: 10.1016/B978-1-4160-4390-4.00103-X.
Rosenthal, BM; Dunams, DB and Pritt, B (2008). Restricted genetic diversity in the ubiquitous cattle parasite, Sarcocystis cruzi. Infect. Genet. Evol., 8: 588-592. doi: 10.1016/j.meegid.2008.04.004.
Sarafraz, N; Spotin, A; Haniloo, and Fazaeli, A (2020). Prevalence and molecular analysis of Sarcocystis infections in cattle in Northwest Iran and the first global report of S. gigantea in cattle. Comp. Immunol. Microbiol. Infect. Dis., 73: 101566. doi: 10.1016/j.cimid.2020.101566.
Shahraki, MK; Ghanbarzehi, A and Dabirzadeh, M (2018). Prevalence and histopathology of Sarcocystosis in slaughtered carcasses in southeast Iran. J. Adv. Vet. Anim. Res., 5: 381-387. doi: 10.5455/javar.2018.e288.
Sheikhi, M; Salahi-Moghaddam, A; Najafi Asl, M; Farahani, A and Shamseddin, J (2020). A survey on the frequency of Sarcocystis in Bandar Abbas, Iran in 2019-2020. Gene Cell Tissue. 7: e109990. doi: 10.5812/gct. 109990.
Shekarforoush, S; Razavi, S and Abbasvali, M (2013). First detection of Sarcocystis hirsuta from cattle in Iran. Iran. J. Vet. Res., 14: 155-157. doi: 10.22099/ijvr. 2013.1591.
Sudan, V; Kumar, R; Shanker, D and Singh, A (2021). Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene. Beni-Suef Univ. J. Basic Appl. Sci., 10: 1-5. doi: 10.1186/s43088-021-00112-z.
Taib, M; Harhoura, K; Aissi, M; Chaouadi, M and Djouhri, Y (2016). Study of the bovine Sarcosporidiosis in the slaughterhouses of the North of Algeria: case of the Slaughterhouses of El Harrach (Algiers). Cell Dev. Biol., 5: 167-175.
Tamura, K; Stecher, G; Peterson, D; Filipski, A and Kumar, S (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 30: 2725-2729. doi: 10.1093/molbev/mst197.
Thompson, JD; Higgins, DG and Gibson, TJ (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22: 4673-4680. doi: 10.1093/nar/22.22. 4673.
Vangeel, L; Houf, K; Geldhof, P; Nollet, H; Vercruysse, J; Ducatelle, R and Chiers, K (2012). Intramuscular inoculation of cattle with Sarcocystis antigen results infocal eosinophilic myositis. Vet. Parasitol., 183: 224-230. doi: 10.1016/j.vet par.2011 .07.048.
Yang, J; Kang, YB; Wee, SH; Lee, S and Kim, K (1990). Prevalence of Sarcocystis infections in the slaughtered cattle in Cheju. Korean J. Vet. Res., 30: 507-510. | ||
|
آمار تعداد مشاهده مقاله: 1,296 تعداد دریافت فایل اصل مقاله: 897 |
||