| تعداد نشریات | 24 |
| تعداد شمارهها | 852 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,420,788 |
| تعداد دریافت فایل اصل مقاله | 11,583,423 |
The effects of embryo splitting on Cdx2, Sox2, Oct4, and Nanog gene expression in mouse blastocysts | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 4 - شماره پیاپی 81، 2022، صفحه 331-336 اصل مقاله (518.75 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.42487.6172 | ||
| نویسندگان | ||
| M. Rahbaran1؛ A. R. Tayefeh2؛ F. Heidari* 2 | ||
| 1MSc in Biotechnology, Animal Biotechnology Department, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Iran | ||
| 2Animal Biotechnology Department, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Iran | ||
| چکیده | ||
| Background: Embryo splitting is utilized in reproduction biotechnology. The blastomeres resulting from the splitting of an embryo in two-, four- or eight-cell stages can develop into separate embryos that are genetically similar to the other blastomeres. Aims: The present work studied the effects of splitting on embryo pluripotent gene expression (Cdx2, Sox2, Oct4, and Nanog) in mice. Methods: Two-cell embryos were isolated from stimulated mice. The embryos were grouped into “split” and “non-split” groups. The zona pellucida was removed from the split group and the blastomeres were distributed before being co-cultured with mouse embryo fibroblasts to the blastocyst stage. Normal (non-split) blastocysts were co-cultured in the same way. The 3.5-day-old blastomeres were collected as the control group. For molecular evaluation, real-time PCR was conducted to analyze changes in Cdx2, Sox2, Oct4, and Nanog gene expression. Moreover, the blastocyst formation rate, overall blastocyst rate, and the number of newborns were statistically analyzed. Results: The findings showed that embryo splitting increased blastocyst formation, overall blastocysts, developmental potential embryos, and the number of infants. Furthermore, the split and non-split (control) groups showed equal expression of pluripotent genes (Cdx2, Sox2, Oct4, and Nanog) in the molecular analysis. Conclusion: It can be concluded that the growth and developmental potency of sister blastocysts derived from split two-cell stage mouse embryos are the same as those of normal blastocysts. So, there are no significant differences in gene expression between the split and non-split groups. | ||
| کلیدواژهها | ||
| Embryo splitting؛ Gene expression؛ Mouse blastocyst؛ Two-cell embryo | ||
| مراجع | ||
|
Aktan, E; Ozer, D; Demirol, A; Gurgan, T and Bozkurt, K (2006). The effect of zona thinning size on implantation and pregnancy rates of ICSI-ET patients with advanced woman age. Middle East Fertil. Soc. J., 11: 183-190.
Boiani, M; Casser, E; Fuellen, G and Christians, ES (2019). Totipotency continuity from zygote to early blastomeres: a model under revision. Reproduction. 158: R49-R65.
Casser, E; Israel, S and Boiani, M (2019). Multiplying embryos: experimental monozygotic polyembryony in mammals and its uses. Int. J. Dev. Biol., 63: 143-155.
Casser, E; Israel, S; Witten, A; Schulte, K; Schlatt, S; Nordhoff, V and Boiani, M (2017). Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos. Sci. Rep., 7: 1-15.
Cimadomo, D; Capalbo, A; Ubaldi, FM; Scarica, C; Palagiano, A; Canipari, R and Rienzi, L (2016). The impact of biopsy on human embryo developmental potential during preimplantation genetic diagnosis. Biomed. Res. Int., 2016: 1-10.
Fallahi, S and Mohammadhassan, R (2020). A review of pharmaceutical recombinant proteins and gene transformation approaches in transgenic poultry. J. Trop. Life Sci., 10: 163-173.
Gardner, DK and Balaban, B (2016). Assessment of human embryo development using morphological criteria in an era of time-lapse, algorithms and ‘OMICS’: is looking good still important?. Mol. Hum. Reprod., 22: 704-718.
Gleicher, N and Orvieto, R (2017). Is the hypothesis of preimplantation genetic screening (PGS) still supportable? A review. J. Ovarian Res., 10: 1-7.
Harrison, SE; Sozen, B; Christodoulou, N; Kyprianou, C and Zernicka-Goetz, M (2017). Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science. 356: eaal1810.
Hendriks, S (2017). Towards the responsible clinical implementation of stem cell-based fertility treatments. Hum. Reprod., 32: 2076-2087.
Illmensee, K; Kaskar, K and Zavos, PM (2006). In vitro blastocyst development from serially split mouse embryos and future implications for human assisted reproductive technologies. Fertil. Steril., 86: 1112-1120.
Kim, K; Lee, K; Bang, H; Kim, JY and Choi, JK (2016). Intersection of genetics and epigenetics in monozygotic twin genomes. Methods. 102: 50-56.
Klimczewska, K; Kasperczuk, A and Suwińska, A (2018). The regulative nature of mammalian embryos. Curr. Top. Dev. Biol., 128: 105-149.
Kuliev, A; Rechitsky, S and Simpson, JL (2020). Strategies and indications for preimplantation genetic testing for monogenic disorders (PGT-M). In: Kuliev, A, Rechitsky, S and Simpson, JL (Eds.), Practical preimplantation genetic testing. (3rd Edn.), Cham, Switzerland, Springer. PP: 49-181.
Menchero, S; de Aja, JS and Manzanares, M (2018). Our first choice: cellular and genetic underpinnings of trophectoderm identity and differentiation in the mammalian embryo. Curr. Top. Dev. Bio., 128: 59-80.
Nissen, SB; Perera, M; Gonzalez, JM; Morgani, SM; Jensen, MH; Sneppen, K; Brickman, JM and Trusina, A (2017). Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Bio., 15: e2000737.
Noli, L (2017). Comparative analyses of twin blastocysts. Reproduction. 23: 156-165.
Piotrowska-Nitsche, K; Perea-Gomez, A; Haraguchi, S and Zernicka-Goetz, M (2005). Four-cell stage mouse blastomeres have different developmental properties. Development. 132: 479-490. Rahbaran, M; Razeghian, E; Maashi, MS; Jalil, AT; Widjaja, G; Thangavelu, L; Kuznetsova, MY; Nasirmoghadas, P; Heidari, F and Marofi, FJSCI (2021). Cloning and embryo splitting in mammalians: brief history, methods, and achievements. Stem. Cells Intern., 2021: 1-11.
Rao, J and Greber, B (2017). Concise review: signaling control of early fate decisions around the human pluripotent stem cell state. Stem. Cells. 35: 277-283.
Rizzino, A and Wuebben, EL (2016). Sox2/Oct4: A delicately balanced partnership in pluripotent stem cells and embryogenesis. Biochim. Biophy. Act. (BBA)-Gen. Regul. Mech., 1859: 780-791.
Sagoskin, AW; Levy, MJ; Tucker, MJ; Richter, KS and Widra, EA (2007). Laser assisted hatching in good prognosis patients undergoing in vitro fertilization-embryo transfer: a randomized controlled trial. Fertil. Steril., 87: 283-287.
Samadian, A; Hesaraki, M; Mollamohammadi, S; Asgari, B; Totonchi, M and Baharvand, H (2018). Temporal gene expression and DNA methylation during embryonic stem cell derivation. Cell J. (Yakhteh). 20: 361-368.
Seidel Jr, GE (2015). Lessons from reproductive technology research. Annu. Rev. Anim. Biosci., 3: 467-487.
Sepulveda-Rincon, LP; Dube, D; Adenot, P; Laffont, L; Ruffini, S; Gall, L; Campbell, BK; Duranthon, V; Beaujean, N and Maalouf, WE (2016). Random allocation of blastomere descendants to the trophectoderm and ICM of the bovine blastocyst. Biol. Reprod., 95: 1-10.
Sun, H; Su, J; Wu, T; Wang, F; Kang, J; Zhang, J; Xing, X; Cheng, Y and Zhang, Y (2020). CARM1 is heterogeneous in mouse four-cell embryo and important to blastocyst development. Reproduction. 159: 91-104.
Takahashi, K and Yamanaka, S (2016). A decade of transcription factor-mediated reprogramming to pluripotency. Nat. Rev. Mol. Cell Bio., 17: 183-193.
Tang, HH; Tsai, YC and Kuo, CT (2012). Embryo splitting can increase the quantity but not the quality of blastocysts. Tai. J. Obst. Gyn., 51: 236-239.
Taşkin, AC; Akkoc, T; Sağirkaya, H; Bağiş, H and Arat, S (2016). Comparison of the development of mouse embryos manipulated with different biopsy techniques. Turk. J. Vet. Anim. Sci., 40: 157-162.
Wang, J; Wang, L; Feng, G; Wang, Y; Li, Y; Li, X; Liu, C; Jiao, G; Huang, C and Shi, J (2018). Asymmetric expression of LincGET biases cell fate in two-cell mouse embryos. Cell. 175: 1887-1901.
Zhu, Y and Lohnes, D (2022). Regulation of axial elongation by Cdx. Dev. Biol., 483: 118-127. | ||
|
آمار تعداد مشاهده مقاله: 683 تعداد دریافت فایل اصل مقاله: 469 |
||