| تعداد نشریات | 24 |
| تعداد شمارهها | 903 |
| تعداد مقالات | 8,081 |
| تعداد مشاهده مقاله | 15,203,533 |
| تعداد دریافت فایل اصل مقاله | 12,847,887 |
Decellularized xenogenic bone graft for repair of segmental bone defect in rabbits | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 4 - شماره پیاپی 81، 2022، صفحه 310-321 اصل مقاله (969.01 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.40785.5906 | ||
| نویسندگان | ||
| P. Tamilmahan* 1؛ R. Pathak2؛ Rashmi Rashmi3؛ Amarpal Amarpal2؛ H. P. Aithal2؛ A. Mohsina3؛ A. K. Tiwari4؛ K. Karthik5 | ||
| 1Ph.D. Student in Veterinary Surgery and Radiology, Division of Veterinary Surgery, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, IndiaAssistant Professor, TANUVAS | ||
| 2Division of Veterinary Surgery, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India | ||
| 3Ph.D. Student in Veterinary Surgery and Radiology, Division of Veterinary Surgery, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India | ||
| 4Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India | ||
| 5Department of Veterinary Microbiology, TANUVAS, Tamil Nadu, India | ||
| چکیده | ||
| Background: Bone grafting is a preferred treatment option for the healing of large diaphyseal bone defects and is useful in the management of nonunion, delayed union, and tumor resection. Aims: To investigate a decellularization protocol of bovine cancellous bone for xenogenic implantation in radial bone defects in rabbits. Methods: Bovine bone scaffolds fabricated with various decellularization protocols viz phosphate buffer saline (PBS), 1% sodium dodecyl sulfate (SDS), and rapid freeze and thaw technique. The manufactured scaffolds were characterized by biomechanical testing, histological staining, and scanning electron microscopy. A 10 mm rabbit radius bone defect was repaired with autograft and SDS treated and rapid freeze and thaw in groups A, B, and C respectively. Healing was evaluated by radiography and histopathology at 0, 30, 60, and 90 days. The grafts were also checked for host tissue reaction and incorporation into the defect. Results: The freeze and thaw group showed complete elimination of all cellular nuclei, regular arrangement of collagen fiber, and no significant difference in tensile strength compared to 1% SDS treated and native groups. The in vivo radiographic and histopathological study showed that the rapid freeze and thaw group had complete bridging of the bone gap defect with new bone formation and they were immunologically less reactive compared to group B. Conclusion: The in vitro and in vivo evaluation of the grafts suggested that freeze and thaw technique was most superior to all other techniques for effective decellularization and augmentation of bone healing with better integration of the graft into the host. | ||
| کلیدواژهها | ||
| Bovine bone graft؛ Bone defect؛ Bone regeneration؛ Decellularization؛ Rapid freeze and thaw | ||
| مراجع | ||
|
Amarpal; Kinjavdekar, P; Aithal, HP; Pawde, AM and Pratap, K (2010). Evaluation of Xylazine, Acepromazine and Medetomidine with Ketamine for general anesthesia in rabbits. Scand. J. Lab. Anim. Sci., 37: 223-229.
Badylak, SF; Taylor, D and Uygun, K (2011). Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu. Rev. Biomed. Eng., 13: 27-53.
Badylak, SF; Valentin, JE; Ravindra, AK; McCabe, GP and Stewart-Akers, AM (2008). Macrophage phenotype as a determinant of biologic scaffold remodeling. Tissue Eng. Part A., 14: 1835-1842.
Borchers, RE; Gibson, LJ; Burchardt, H and Hayes, WC (1995). Effects of selected thermal variables on the mechanical properties of trabecular bone. Biomaterials. 16: 545-551.
Caporali, EHG; Sheila, CR; Morceli, J; Taga, R; Mauro, JO; Tania, GM; Maria, C; Mamprim, J and Mariana, AC (2006). Assessment of bovine biomaterials containing bone morphogenetic proteins bound to absorbable hydroxyapatite in rabbit segmental bone defects. Acta. Cir. Bras., 21: 367-373.
Chan, LK; Leung, VY; Tam, V; Lu, WW; Sze, KY and Cheung, KM (2013). Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies. Acta. Biomater., 9: 5262-5272.
Cheng, CW; Solorio, LD and Alsberg, E (2014). Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering. Biotechnol. Adv., 32: 462-484.
Elder, BD; Eleswarapu, SV and Athanasiou, KA (2009). Extraction techniques for the decellularization of tissue engineered articular cartilage constructs. Biomaterials. 30: 3749-3756.
Elder, BD; Kim, DH and Athanasiou, KA (2010). Developing an articular cartilage decellularization process toward facet joint cartilage replacement. Neurosurgery. 66: 722-727.
Elsaesser, AF; Bermueller, C; Schwarz, S; Koerber, L; Breiter, R and Rotter, N (2014). In vitro cytotoxicity and in vivo effects of a decellularized xenogeneic collagen scaffold in nasal cartilage repair. Tissue Eng. Part A., 20: 1668-1678.
Frohlich, M; Grayson, WL; Marolt, D; Gimble, JM; Kregar-Velikonja, N and Vunjak-Novakovic, G (2010). Bone grafts engineered from human adipose-derived stem cells in perfusion bioreactor culture. Tissue Eng. Part A., 16: 179-189.
Gardin, C; Ricci, S; Ferroni, L; Guazzo, R; Sbricoli, L; De Benedictis, G; Finotti, L; Isola, M; Bressan, E and Zavan, B (2015). Decellularization and delipidation protocols of bovine bone and pericardium for bone grafting and guided bone regeneration procedures. PLoS One. 10: e0132344.
Gilbert, TW; Freund, J and Badylak, SF (2009). Quantification of DNA in biological scaffold materials. J. Surg. Res., 152: 135-139.
Gock, H; Murray-Segal, L; Salvaris, E; Cowan, PD and Apice, AJ (2004). Allogeneic sensitization is more effective than xenogeneic sensitization in eliciting Gal-mediated skin graft rejection. Transplantation. 77: 751-753.
Guo, L; Qu, J; Zheng, C; Cao, Y; Zhang, T; Lu, H and Hu, J (2015). Preparation and characterization of a novel decellularized fibrocartilage “book” scaffold for use in tissue engineering. PLoS One. 4: e0144240.
Heiple, KG; Goldberg, VM; Powell, AE; Bos, GD and Zika, JM (1987). Biology of cancellous bone grafts. Orthop. Clin. North. Am., 18: 179-185.
Jonitz, A; Lochner, K; Lindner, T; Hansmann, D; Marrot, A and Bader, R (2011). Oxygen consumption, acidification and migration capacity of human primary osteoblasts within a three-dimensional tantalum scaffold. J. Mater. Sci. Mater. Med., 22: 2089-2095.
Kalab, M; Karkoska, J; Kaminek, M and Santavy, P (2015). Successful three-year outcome in a patient with allogenous sternal bone graft in the treatment of massive post-sternotomy defects. Int. J. Surg. Case Rep., 7: 6-9.
Karalashvili, L; Chichua, N; Menabde, G; Atskvereli, L and Grdzelidze, T (2017). Decellularized bovine bone graft for zygomatic bone reconstruction. Med. Case Rep., 4: 1-5.
Kruisbeek, AM; Shevach, E and Thornton, AM (2004). Proliferative assays for T cell function. Curr. Protoc. Immunol., 60: 3-12.
Lane, JM and Sandhu, HS (1987). Current approach to experimental bone grafting. Orthop. Clin. North Am., 18: 213-225.
Lee, DJ; Diachina, S; Lee, YT; Zhao, L; Zou, R; Tang, N; Han, H; Chen, X and Ko, CC (2016). Decellularized bone matrix grafts for calvaria regeneration. J. Tissue Eng., 7: 1-11.
Lehr, EJ; Rayat, GR; Chiu, B; Churchill, T; McGann, LE and Coe, JY (2011). Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches. J. Thorac. Cardiovasc. Surg., 141: 1056-1062.
Ma, R; Li, M; Luo, J; Yu, H; Sun, Y; Cheng, S and Cui, P (2013). Structural integrity, ECM components and immunogenicity of decellularized laryngeal scaffold with preserved cartilage. Biomaterials. 34: 1790-1798.
Mollon, B; Kandel, R; Chahal, J and Theodoropoulos, J (2013). The clinical status of cartilage tissue regeneration in humans. Osteoarthr. Cartil., 21: 1824-1833.
Nandi, SK; Kundu, B; Ghosh, SK; De, DK and Basu, D (2008). Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat. J. Vet. Sci., 9: 183-191.
Oryan, A; Alidadi, S; Moshiri, A and Maffulli, N (2014). Bone regenerative medicine: classic options, novel strategies, and future directions. J. Orthop. Surg. Res., 9: 1-27.
Peng, F; Yu, X and Wei, M (2011). In vitro cell performance on hydroxyapatite particles/poly (L-lactic acid) nanofibrous scaffolds with an excellent particle along nanofiber orientation. Acta. Biomater., 7: 2585-2592.
Quan, TM; Vu, DN; My, NT and Ha, TL (2014). Decellularization of xenogenic bone grafts for potential use as tissue engineering scaffolds. Int. J. Life Sci. Med. Res., 4: 38-45.
Remya, V; Kumar, N; Sharma, AK; Mathew, DD; Negi, M; Maiti, SK; Shrivastava, S and Kurade, NP (2014). Bone marrow derived cell-seeded extracellular matrix: A novel biomaterial in the field of wound management. Vet. World. 7: 1019-1025.
Sambrook, J and Russell, DW (2001). Molecular cloning- a laboratory manual. 3rd Edn., New York, Cold Spring Harbor Laboratories Press, Cold Spring Harbor.
Soltz, MA and Ateshian, GA (2000). A conewise linear elasticity mixture model for the analysis of tension-compression nonlinearity in articular cartilage. J. Biomech. Eng., 122: 576-586.
Somerman, M; Hewitt, AT; Varner, HH; Schiffmann, E; Termine, J and Reddi, AH (1983). Identification of a bone matrix-derived chemotactic factor. Calcif. Tissue Internat., 35: 481-485.
Stievano, D; Di Stefano, A; Ludovichetti, M; Pagnutti, S; Gazzola, F; Boato, C and Stellini, E (2008). Maxillary sinus lift through heterologous bone grafts and simultaneous acid-etched implants placement. Minerva. Chir., 63: 79-91. Sullivan, DC; Mirmalek-Sani, SH; Deegan, DB; Baptista, PM; Aboushwareb, T; Atala, A and Yoo, JJ (2012). Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system. Biomaterials. 33: 7756-7764.
Teo, KY; DeHoyos, TO; Dutton, JC; Grinnell, F and Han, B (2011). Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. Biomaterials. 32: 5380-5390.
Udehiya, RK; Aithal, HP; Kinjavdekar, P; Pawde, AM; Singh, R and Sharma, GT (2013). Comparison of autogenic and allogenic bone marrow derived mesenchymal stem cells for repair of segmental bone defects in rabbits. Vet. Sci. Res. J., 94: 743-752.
Ventura, R; Padalhin, A; Young-Ki, M and Byong-Taek, L (2016). Bone regeneration of decellularized in-vivo deposited extracellular matrix (ECM) on hydroxyapatite sponge scaffold. MOJ Cell Sci. Rep., 3: 3-6.
Woods, T and Gratzer, PF (2005). Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament bone graft. Biomaterials. 26: 7339-7349.
Xing, Q; Yates, K; Tahtinen, M; Shearier, E; Qian, Z and Zhao, F (2015). Decellularization of fibroblast cell sheets for natural extracellular matrix scaffold preparation. Tissue Eng. Part C Methods. 21: 77-87.
Yildirim, M; Spiekermann, H; Handt, S and Edelhoff, D (2001). Maxillary sinus augmentation with the xenograft Bio-Oss® and autogenous intraoral bone for qualitative improvement of the implant site: a histologic and histomorphometric clinical study in humans. Int. J. Oral Maxillo Fac. Implants. 16: 23-33.
You, L; Weikang, X; Lifeng, Y; Changyan, L; Yongliang, L; Xiaohui, W and Bin, X (2018). In vivo immunogenicity of bovine bone removed by a novel decellularization protocol based on supercritical carbon dioxide. Artif. Cells Nanomed. Biotechnol., 5: 334-344.
Zheng, MH; Chen, J; Kirilak, Y; Willers, C; Xu, J and Wood, D (2005). Porcine small intestine submucosa (SIS) is not an acellular collagenous matrix and contains porcine DNA: possible implications in human implantation. J. Biomed. Mater. Res. B Appl. Biomater., 73: 61-67. | ||
|
آمار تعداد مشاهده مقاله: 985 تعداد دریافت فایل اصل مقاله: 667 |
||