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Husseini, Abbas Ali, Rostamzadeh, Mehran. (1401). Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. , 11(4), 183-186. doi: 10.22099/mbrc.2022.44692.1780
Abbas Ali Husseini; Mehran Rostamzadeh. "Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan". , 11, 4, 1401, 183-186. doi: 10.22099/mbrc.2022.44692.1780
Husseini, Abbas Ali, Rostamzadeh, Mehran. (1401). 'Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan', , 11(4), pp. 183-186. doi: 10.22099/mbrc.2022.44692.1780
Husseini, Abbas Ali, Rostamzadeh, Mehran. Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. , 1401; 11(4): 183-186. doi: 10.22099/mbrc.2022.44692.1780

Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan

Molecular Biology Research Communications
دوره 11، شماره 4، 2022، صفحه 183-186    اصل مقاله (140.1 K)
نوع مقاله: Short communication
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.44692.1780
نویسندگان
Abbas Ali Husseini* ؛ Mehran Rostamzadeh
Life Science, and Biomedical Engineering Application and Research Center, Istanbul Gelisim University, Istanbul 34310, Turkey
چکیده
The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program.  The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepa­titis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan.
کلیدواژه‌ها
Hepatitis Delta؛ genotype؛ Phylogenetic analysis؛ Afghanistan
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