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Zehtabi, Mojtaba, Akbarpour, Zahra, Valizadeh, Sepehr, Roosta, Yousef, Khamaneh, Amir Mahdi, Raeisi, Mortaza. (1401). Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway. , 11(4), 173-181. doi: 10.22099/mbrc.2022.43844.1751
Mojtaba Zehtabi; Zahra Akbarpour; Sepehr Valizadeh; Yousef Roosta; Amir Mahdi Khamaneh; Mortaza Raeisi. "Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway". , 11, 4, 1401, 173-181. doi: 10.22099/mbrc.2022.43844.1751
Zehtabi, Mojtaba, Akbarpour, Zahra, Valizadeh, Sepehr, Roosta, Yousef, Khamaneh, Amir Mahdi, Raeisi, Mortaza. (1401). 'Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway', , 11(4), pp. 173-181. doi: 10.22099/mbrc.2022.43844.1751
Zehtabi, Mojtaba, Akbarpour, Zahra, Valizadeh, Sepehr, Roosta, Yousef, Khamaneh, Amir Mahdi, Raeisi, Mortaza. Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway. , 1401; 11(4): 173-181. doi: 10.22099/mbrc.2022.43844.1751

Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway

Molecular Biology Research Communications
دوره 11، شماره 4، 2022، صفحه 173-181    اصل مقاله (222.04 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.43844.1751
نویسندگان
Mojtaba Zehtabi1؛ Zahra Akbarpour2؛ Sepehr Valizadeh3؛ Yousef Roosta4؛ Amir Mahdi Khamaneh5؛ Mortaza Raeisi* 1
1Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Rahat Breathe and Sleep Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Internal Medicine, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Internal Medicine, School of Medicine, Imam Khomeini Hospital, Urmia University of Medical Sciences
5Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Papillary thyroid carcinoma (PTC) is the most common endocrine cancer. However, the role of biomechanics in the development and progression of PTC is obscure. The microarray dataset GSE104005 was examined to identify important microRNAs (miRNAs or miRs) and their probable roles in the carcinogenesis of PTC. The gene expression omnibus (GEO) database was used to obtain the data. R was used to access the differentially expressed miRNAs (DEMs) and genes (DEGs). The multiMiR software was used to predict DEM targets. To validate the top DEMs and DEGs, thirty tissue samples were obtained from PTC patients who had their thyroids removed and compared with 30 normal samples. The total RNA content of the tumor and corresponding non-tumoral adjacent samples were purified and converted to cDNA. Expression levels of top dysregulated miRNAs and their target and predicted DEG were evaluated using the RT-qPCR method. miR-182 and miR-183 were top upregulated miRs and miR-30d was the most downregulated miR among DEMs. Furthermore, FOXO1 which was shown to be targeted by aforementioned miRNAs, was the most downregulated genes among other DEGs. 10 hub nodes were detected by PPI construction. PTEN was the hub node with highest score. The in vitro gene expression analysis was also showed the same expression pattern in tissues. Significant increase in miR-182-5p and miR-183-5p expressions, as well as a significant decrease in FOXO1 and miR-30d-5p expressions, suggest that PTC cells may tend to preserve their autophagy capability.
کلیدواژه‌ها
Papillary thyroid carcinoma؛ Autophagy؛ mRNA؛ miRNA
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مراجع
  1. Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA: A Cancer J Clinic 2014;64:9-29.
  2. Milano AF. Thyroid Cancer: 20-Year comparative mortality and survival analysis of six thyroid cancer histologic subtypes by age, sex, race, stage, cohort entry time-period and disease duration (SEER*Stat 8.3.2) A systematic review of 145,457 cases for diagnosis years 1993-2013. J Insur Med 2018;47:143-158.
  3. Aschebrook-Kilfoy B, Ward MH, Sabra MM, Devesa SS. Thyroid cancer incidence patterns in the United States by histologic type, 1992-2006. Thyroid 2011;21:125-134.
  4. Valizadeh S, Zehtabi M, Feiziordaklou N, Akbarpour Z, Khamaneh AM, Raeisi M. Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. Mol Biol Res Commun 2022;11:133-141.
  5. Kitahara CM, Sosa JA. Understanding the ever-changing incidence of thyroid cancer. Nat Rev Endocrinol 2020;16:617-618.
  6. Arianpoor A, Asadi M, Amini E, Ziaeemehr A, Ahmadi Simab S, Zakavi SR. Investigating the prevalence of risk factors of papillary thyroid carcinoma recurrence and disease-free survival after thyroidectomy and central neck dissection in Iranian patients. Acta Chir Belg 2020;120:173-178.
  7. Peng Y, Croce CM. The role of MicroRNAs in human cancer. Signal Transduct Target Ther 2016;1:15004.
  8. Garzon R, Fabbri M, Cimmino A, Calin GA, Croce CM. MicroRNA expression and function in cancer. Trends Mol Med 2006;12:580-587.
  9. Yang X, Zhong X, Tanyi JL, Shen J, Xu C, Gao P, Zheng TM, DeMichele A, Zhang L. mir-30d Regulates multiple genes in the autophagy pathway and impairs autophagy process in human cancer cells. Biochem and Biophys Res Commun 2013;431:617-622.
  10. Sarver AL, Sarver AE, Yuan C, Subramanian S. OMCD: OncomiR cancer database. BMC Cancer 2018;18:1223.
  11. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25:402-408.
  12. Xu Y, Tian Y, Li F, Wang Y, Yang J, Gong H, Wan X, Ouyang M. Circular RNA HECTD1 mitigates ulcerative colitis by promoting enterocyte autophagy via miR-182-5p/HuR axis. Inflamm Bowel Dis 2022;28:273-288.
  13. Peng X, Li W, Yuan L, Mehta RG, Kopelovich L, McCormick DL. Inhibition of proliferation and induction of autophagy by atorvastatin in PC3 prostate cancer cells correlate with downregulation of Bcl2 and upregulation of miR-182 and p21. PLoS One 2013;8:e70442.
  14. Wei Y, Hong D, Zang A, Wang Z, Yang H, Zhang P, Wang Y. miR-183 enhances autophagy of GC cells by targeted inhibition of mTOR. Ann Clin Lab Sci 2021;51:837-843.
  15. Li H, He C, Wang X, Wang H, Nan G, Fang L. MicroRNA-183 affects the development of gastric cancer by regulating autophagy via MALAT1-miR-183-SIRT1 axis and PI3K/AKT/mTOR signals. Artif Cells Nanomed Biotechnol 2019;47:3163-3171.
  16. Zhao F, Qu Y, Wang H, Huang L, Zhu J, Li S, Tong Y, Zhang L, Li J, Mu D. The effect of miR-30d on apoptosis and autophagy in cultured astrocytes under oxygen-glucose deprivation. Brain Res 2017;1671:67-76.
  17. Liang L, Yang Z, Deng Q, Jiang Y, Cheng Y, Sun Y, Li L. miR-30d-5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma. FEBS Open Bio 2021;11:529-540.
  18. Zhao Y, Yang J, Liao W, Liu X, Zhang H, Wang S, Feng J, Yu L, Zhu WG. Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Nat Cell Biol 2010;12:665-675.
  19. Xie Y, Li X, Ge J. Cyclophilin A-FoxO1 signaling pathway in endothelial cell apoptosis. Cell Signal 2019;61:57-65.
  20. Zheng K, He Z, Kitazato K, Wang Y. Selective autophagy regulates Cell Cycle in Cancer Therapy. Theranostics 2019;9:104-125.
  21. Levy JMM, Towers CG, Thorburn A. Targeting autophagy in cancer. Nat Rev Cancer 2017;17:528-542.
  22. Bartha Á, Győrffy B. TNMplot.com: A web tool for the comparison of gene expression in normal, tumor and metastatic tissues. Int J Mol Sci 2021;22:2622.
  23. Wen X, Klionsky DJ. At a glance: A history of autophagy and cancer. Semin Cancer Biol 2020;66:3-11.
  24. Yun CW, Lee SH. The roles of autophagy in cancer. Int J Mol Sci 2018;19:3466.
  25. Chavez-Dominguez R, Perez-Medina M, Lopez-Gonzalez JS, Galicia-Velasco M, Aguilar-Cazares D. The double-edge sword of autophagy in cancer: From tumor suppression to pro-tumor activity. Front Oncol 2020;10:578418.
  26. Myatt SS, Wang J, Monteiro LJ, Christian M, Ho KK, Fusi L, Dina RE, Brosens JJ, Ghaem-Maghami S, Lam E. Definition of microRNAs that repress expression of the tumor suppressor gene FOXO1 in endometrial cancer. Cancer Res 2010;70:367-377.
  27. Ma Y, Liang AJ, Fan YP, Huang YR, Zhao XM, Sun Y, Feng Chen X. Dysregulation and functional roles of miR-183-96-182 cluster in cancer cell proliferation, invasion and metastasis. Oncotarget 2016;7:42805-42825.
  28. Yoshii SR, Mizushima N. Monitoring and measuring autophagy. Int J Mol Sci 2017;18: 1865.
  29. Zheng S, Zhong YF, Tan DM, Xu Y, Chen HX, Wang D. miR-183-5p enhances the radioresistance of colorectal cancer by directly targeting ATG5. J Biosci 2019;44:92.
  30. Kim HM, Kim ES, Koo JS. Expression of autophagy-related proteins in different types of thyroid cancer. Int J Mol Sci 2017;18:540.
  31. Liu H, Chen X, Lin T, Chen X, Yan J, Jiang S. MicroRNA-524-5p suppresses the progression of papillary thyroid carcinoma cells via targeting on FOXE1 and ITGA3 in cell autophagy and cycling pathways. J Cell Physiol 2019;234:18382-18391.
  32. Shen H, Lin Z, Shi H, Wu L, Ma B, Li H, Yin B, Tang J, Yu H, Yin X. MiR-221/222 promote migration and invasion, and inhibit autophagy and apoptosis by modulating ATG10 in aggressive papillary thyroid carcinoma. 3 Biotech 2020;10:339.
  33. Zhao Y, Zhao L, Li J, Zhong L. Silencing of long noncoding RNA RP11-476D10.1 enhances apoptosis and autophagy while inhibiting proliferation of papillary thyroid carcinoma cells via microRNA-138-5p-dependent inhibition of LRRK2. J Cell Physiol 2019;234:20980-20991.
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