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Dara, Mahintaj, Habibi, Azam, Azarpira, Negar, Dianatpour, Mehdi, Nejabat, Mahmood, Khosravi, Amir, Tanideh, Nader. (1401). Novel RNA extraction method from human tears. , 11(4), 167-172. doi: 10.22099/mbrc.2022.45266.1801
Mahintaj Dara; Azam Habibi; Negar Azarpira; Mehdi Dianatpour; Mahmood Nejabat; Amir Khosravi; Nader Tanideh. "Novel RNA extraction method from human tears". , 11, 4, 1401, 167-172. doi: 10.22099/mbrc.2022.45266.1801
Dara, Mahintaj, Habibi, Azam, Azarpira, Negar, Dianatpour, Mehdi, Nejabat, Mahmood, Khosravi, Amir, Tanideh, Nader. (1401). 'Novel RNA extraction method from human tears', , 11(4), pp. 167-172. doi: 10.22099/mbrc.2022.45266.1801
Dara, Mahintaj, Habibi, Azam, Azarpira, Negar, Dianatpour, Mehdi, Nejabat, Mahmood, Khosravi, Amir, Tanideh, Nader. Novel RNA extraction method from human tears. , 1401; 11(4): 167-172. doi: 10.22099/mbrc.2022.45266.1801

Novel RNA extraction method from human tears

Molecular Biology Research Communications
دوره 11، شماره 4، 2022، صفحه 167-172    اصل مقاله (269.09 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.45266.1801
نویسندگان
Mahintaj Dara* 1؛ Azam Habibi2؛ Negar Azarpira3؛ Mehdi Dianatpour1، 4؛ Mahmood Nejabat5؛ Amir Khosravi5؛ Nader Tanideh1
1Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of tissue engineering and cell therapy, School of Advanced Technology in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran
5Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Human tears can be used as a noninvasive source of genetic materials and biomarkers in the prognosis and diagnosis of ocular and non-ocular diseases. The present protocol is a novel direct RNA extraction method from tears. This study aims to provide a suitable method for direct extraction of RNA from tears with high quality and quantity. In this study, we develop a TRIzol base protocol for direct RNA extraction from human tears. quality and quantity of extracted RNA measured by calculation of 260/280 UV absorption ratio using Nanodrop and real-time PCR. RNA was extracted with this modified method and a purified (260/280 UV absorption ratio between 1.8 to 2 and a high yield of total RNA, on average 95 μg, from tears was extracted. In conclusion, we developed an easy and suitable method for direct extraction of total RNA from tears with high quality and quantity.
کلیدواژه‌ها
RNA؛ Extraction method؛ Human tears؛ Biomarkers
مراجع
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