| تعداد نشریات | 24 |
| تعداد شمارهها | 862 |
| تعداد مقالات | 7,672 |
| تعداد مشاهده مقاله | 13,668,447 |
| تعداد دریافت فایل اصل مقاله | 11,743,013 |
Detection of virulence associated genes in Streptococcus agalactiae isolated from bovine mastitis | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 275-279 اصل مقاله (331.45 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6822 | ||
| نویسندگان | ||
| D. K. Parasana1؛ B. B. Javia* 2؛ D. T. Fefar3؛ D. B. Barad2؛ S. N. Ghodasara2 | ||
| 1MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, India | ||
| 2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, India | ||
| 3Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, India | ||
| چکیده | ||
| Background: Mastitis is one of the most expensive diseases in the dairy industry. It causes heavy monetary losses by decreasing milk production and treatment cost. Streptococcus agalactiae, the cause of contagious bovine mastitis, possesses various virulence factors that contribute to pathogenicity. Aims: The main aim of the study was to evaluate the distribution of virulence genes of S. agalactiae. Methods: In the current work, 98 Streptococcus species were isolated from 320 milk samples, collected from Veterinary Clinical Complex, Junagadh. Out of the isolates, 42 S. agalactiae isolates were used for virulence genes detection. Results: All Streptococcus spp. were confirmed at genus level by targeting tuf gene, and S. agalactiae was identified at species level by targeting 16S rRNA gene. For virulence gene detection, scpB, cfb, and cylE genes were targeted. Of 42 S. agalactiae isolates, 15, 16, and 10 isolates possessed scpB, cfb, and cylE genes, respectively. Conclusion: This study aids us to know virulence characteristics and mechanisms responsible for the development of new strains in mastitis epidemiology in response to prevention and control strategies. | ||
| کلیدواژهها | ||
| Bovine mastitis؛ Molecular detection؛ S. agalactiae؛ Virulence genes | ||
| مراجع | ||
|
Abd Alkader, FS and Hyyawi, SM (2021). Isolation and identification of GBS bacteria from mastitis by CAMP test and Lancefield’s serological grouping. Plant Arch., 21: 770-773.
Amosun, E; Ajuwape, A and Adetosoye, AI (2010). Bovine streptococcal mastitis in southwest and northern states of Nigeria. Afr. J. Biomed. Res., 13: 33-37.
Atyabi, N; Vodjgani, M; Gharagozloo, F and Bahonar, A (2006). Prevalence of bacterial mastitis in cattle from the farms around Tehran. Iran. J. Vet. Res., 7: 76-79.
Beckmann, C; Waggoner, JD and Harris, TO (2002). Identification of novel adhesins from group B streptococci by use of phage display reveals that C5a peptidase mediates fibronectin binding. Infect. Immun., 70: 2869-2876.
Bergseng, H; Lars, B; Marite, R and Kare, B (2007). Real-time PCR targeting the sip gene for detection of group B Streptococcus colonization in pregnant women at delivery. J. Med. Microbiol., 56: 223-228.
Carvalho-Castro, GA; Silva, JR; Paiva, LV; Custódio, DA; Moreira, RO; Mian, GF; Prado, IA; Chalfun-Junior, A and Costa, GM (2017). Molecular epidemiology of Streptococcus agalactiae isolated from mastitis in Brazilian dairy herds. Braz. J. Microbiol., 48: 551-559.
Dad, RK; Shakoor, A; Avais, M; Muhammad, G and Hussain, R (2007). Serology based immunological cross reactivity among various isolates of Streptococcus agalactiae from mastitic buffaloes. Ital. J. Anim. Sci., 6: 865-868.
Ding, Y; Zhao, J; He, X; Li, M; Guan, H; Zhang, Z and Li, P (2016). Antimicrobial resistance and virulence-related genes of Streptococcus obtained from dairy cows with mastitis in Inner Mongolia, China. Pharm. Biol., 54: 162-167.
Dmitriev, A; Shakleina, E and Tkacikova, L (2002). Genetic heterogeneity of the pathogenic potentials of human and bovine group B Streptococci. Folia Microbiol., 47: 291-295.
Dmitriev, A; Suvorov, A; Shen, A and Yang, H (2004). Clinical diagnosis of group B streptococci by scpB gene based PCR. Indian J. Med. Res., 199: 233-236.
Doran, KS; Liu, G and Nizet, V (2003). Group B streptococcal β-hemolysin/cytolysin activates neutrophil signaling pathways in brain endothelium and contributes to development of meningitis. J. Clin. Investig., 112: 736-744.
El-Jakee, J; Hableel, HS; Kandil, M; Hassan, OF; Khairy, EA and Marouf, SA (2013). Antibiotic resistance patterns of Streptococcus agalactiae isolated from mastitic cows and ewes in Egypt. Glob. Vet., 10: 264-270.
Elkenany, R (2020). CylE and mig as virulence genes of streptococci isolated from mastitis in cows and buffaloes in Egypt. Mansoura Vet. Med. J., 21: 149-154.
FDA (2001). Bacteriological Analytical Manual. Food and Drug Administration (FDA). Cited from http://www.scribd. com, Accessed on 08.02.2021.
Ghose, B and Sharda, R (2004). Streptococcal mastitis in dairy cows. Indian Vet. Med. J., 28: 163-164.
Harley, JP and Prescott, LM (2002). Laboratory exercises in microbiology. 5th Edn., Chapter 30: Proteins, amino acids, and Enzymes VII: Oxidase test. New York, USA, The McGraw-Hill Co., PP: 179-184.
Jain, B; Tewari, A; Bhandari, BB and Jhala, MK (2012). Antibiotic resistance and virulence genes in Streptococcus agalactiae isolated from cases of bovine subclinical mastitis. Vet. Arh., 82: 423-432.
Javia, BB; Mathapati, BS; Barad, DB; Ghodasara, SN; Savsani, HH; Bhadaniya, AR; Fefar, DT; Patel, UD and Sindhi, SH (2020). Bacteriological and molecular detection with antimicrobial resistance pattern of major Streptococcus spp. isolated from bovine mastitis. Int. J. Curr. Microbiol. App. Sci., 9: 2443-2451.
Javia, BB; Purohit, JH; Mathapati, BS; Barad, DB; Savsani, HH; Ghodasara, SN; Kalariya, VA; Patel, UD and Nimavat, VR (2018). Molecular detection and antimicrobial resistance pattern of Staphylococci isolated from clinical and subclinical bovine mastitis. Indian J. Vet. Sci. Biotechnolo., 14: 13-16.
Kia, G; Mehdi, G and Keyvan, R (2014). Prevalence and antibiotic susceptibility of Streptococcus spp. in cows with mastitis in Germi, Iran. Anim. Vet. Sci., 2: 31-35.
Nithinprabhu, K (2010). Isolation, characterization and genetic diversity of Streptococcus species in subclinical bovine mastitis. MVSc Thesis, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, India. P: 53.
Picard, FJ; Ke, D; Boudreau, DK; Boissinot, M; Huletsky, A; Richard, D; Ouellette, M; Roy, PH and Bergeron, MG (2004). Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species. J. Clin. Microbiol., 42: 3686-3695. Radtke, A; Bruheim, T; Afset, JE and Bergh, K (2012). Multiple-locus variant-repeat assay (MLVA) is a useful tool for molecular epidemiologic analysis of Streptococcus agalactiae strains causing bovine mastitis. Vet. Microbiol., 157: 398-404.
Richards, VP; Lang, P; Bitar, PDP; Lefebure, T; Schukken, YH; Zadoks, RN and Stanhope, MJ (2011). Comparative genomics and the role of lateral gene transfer in the evolution of bovine adapted Streptococcus agalactiae. Infect. Genet. Evol., 11: 1263-1275.
Riffon, R; Sayasith, K; Khalil, H; Dubreuil, P; Drolet, M and Lagace, J (2001). Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J. Clin. Microbiol., 39: 2584-2589.
Sandholm, M; Honkanen-Buzalski, L; Kaartinen, S and Pyörälä, S (1995). Isolation and identification of pathogens from milk. In: The bovine udder and mastitis. Jyväskylä, Finland, Gummerus Press. PP: 121-141.
Shrestha, S and Bindari, YR (2012). Prevalence of subclinical mastitis among dairy cattle in Bhaktapur District, Nepal. Int. J. Agric. Biosci., 1: 16-19.
Yang, Y; Liu, Y; Ding, Y; Yi, L; Ma, Z; Fan, H and Lu, C (2013). Molecular characterization of Streptococcus agalactiae isolated from bovine mastitis in Eastern China. PLoS One. 8: e6775. | ||
|
آمار تعداد مشاهده مقاله: 378 تعداد دریافت فایل اصل مقاله: 325 |
||