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Biocontrol of Escherichia coli and Salmonella in poultry meat using phage cocktail | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 270-274 اصل مقاله (319.25 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6821 | ||
| نویسندگان | ||
| K. Poojari1؛ D. S. Akhila* 1؛ J. R. Mohan Raj1؛ K. S. Santhosh2؛ A. Kenjar2؛ P. Ashwath1 | ||
| 1Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru, Karnataka 575018, India | ||
| 2Virus Research and Diagnostic Laboratory (VRDL), Shimoga Institute of Medical Sciences (SIMS), Shivamogga-577 201, India | ||
| چکیده | ||
| Background: Despite advances in food management techniques, foodborne illness remains a major concern. Contamination of Salmonella and Escherichia coli pathogens, especially in the poultry sector, is responsible for salmonellosis and other gastrointestinal illness, leading to millions of deaths worldwide. Overuse of antibiotics and other chemical treatments have further increased the emergence of antibiotic resistant bacteria. Aims: This study aimed to study the efficacy of phages cocktail to reduce the load of E. coli and Samlonella spiked on poultry meat. Methods: In this study, a broad spectrum cocktail of phages was used to lyse E. coli and Salmonella spiked on chicken meat. Results: Based on the result of the CFU drop assay, phages like E. coli 153T 3ii and Salmonella 191(3) were selected. Phage concentration of 0.01 MOI showed a reduction in E. coli and Salmonella count to 6 h and 2 h, respectively. Further, phages were tested on the surface of chicken meat. E. coli showed a 90% reduction up to 4 h, whereas Salmonella showed a 90% reduction up to 6 h. When phages were treated in combination, a significant reduction of up to 12 h was found with Salmonella phage, showing better antimicrobial activity. Conclusion: The suitable concentration of a specific phage or phage cocktail can significantly reduce the bacterial count on chicken meat. Phage mediated biocontrol can be used as an alternative approach to eliminate enteric pathogens in the poultry industry. | ||
| کلیدواژهها | ||
| Biocontrol؛ Escherichia coli؛ Phage؛ Poultry؛ Salmonella | ||
| مراجع | ||
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Abedon, ST; Garcia, P; Mullany, P and Aminov, R (2017). Phage therapy: past, present and future. Front. Microbiol., 8: 1-7.
Ahmad, SR; Kalam, A and Ghosh, P (2021). Biocontrol effect of lytic bacteriophages against various foodborne diseases. Biomed. Pharmacol. J., 14: 709-724.
Apostolakos, I and Piccirillo, A (2018). A review on the current situation and challenges of colistin resistance in poultry production. Avian Pathol., 47: 546-558.
Bantawa, K; Sah, SN; Limbu, DS; Subba, P and Ghimire, A (2019). Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal. BMC Res. Notes. 12: 1-6.
Bardina, C; Spricigo, DA; Cortes, P and Llagostera, M (2012). Significance of the bacteriophage treatment schedule in reducing Salmonella colonization of poultry. Appl. Environ. Microbiol., 78: 6600-6607.
Caniça, M; Manageiro, V; Abriouel, H; Moran-Gilad, J and Franz, CM (2019). Antibiotic resistance in foodborne bacteria. Trends Food Sci. Technol., 84: 41-44.
Carter, CD; Parks, A; Abuladze, T; Li, M; Woolston, J; Magnone, J; Senecal, A; Kropinski, AM and Sulakvelidze, A (2012). Bacteriophage cocktail significantly reduces Escherichia coli O157: H7 contamination of lettuce and beef, but does not protect against recontamination. Bacteriophage. 2: 178-185.
Carvalho, CM; Gannon, BW; Halfhide, DE; Santos, SB; Hayes, CM; Roe, JM and Azeredo, J (2010) The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickens. BMC Microbiol., 10: 1-11.
Davis, GS; Waits, K; Nordstrom, L; Grande, H; Weaver, B; Papp, K; Horwinski, J; Koch, B; Hungate, BA; Liu, CM and Price, L (2018). Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims. BMC Microbiol., 18: 1-7.
Duc, HM; Son, M; Honjoh, KI and Miyamoto, T (2018). Isolation and application of bacteriophages to reduce Salmonella contamination in raw chicken meat. Lebensm. Wiss. Technol., 91: 353-360.
EFSA Panel on Biological Hazards (BIOHAZ), EFSA Panel on Contaminants in the Food Chain (CONTAM) and EFSA Panel on Animal Health and Welfare (AHAW) (2012). Scientific opinion on the public health hazards to be covered by inspection of meat (poultry). EFSA J., 10(6): 2741: 1-179.
El-Dougdoug, NK; Cucic, S; Abdelhamid, AG; Brovko, L; Kropinski, AM; Griffiths, MW and Anany, H (2019). Control of Salmonella Newport on cherry tomato using a cocktail of lytic bacteriophages. Int. J. Food Microbiol., 293: 60-71.
Hudson, JA; Billington, C; Wilson, T and On, L (2015). Effect of phage and host concentration on the inactivation of Escherichia coli O157: H7 on cooked and raw beef. Food Sci. Technol. Int., 21: 104-109.
Ishaq, A; Ebner, PD; Syed, QA and Rahman, HU (2020). Employing list-shield bacteriophage as a bio-control intervention for Listeria monocytogenes from raw beef surface and maintain meat quality during refrigeration storage. LWT., 132: 109784.
Kazi, M and Annapure, US (2016). Bacteriophage biocontrol of foodborne pathogens. J. Food Sci. Technol., 53: 1355-1362.
Lee, S; Kim, MG; Lee, HS; Heo, S; Kwon, M and Kim, G (2017). Isolation and characterization of Listeria phages for control of growth of Listeria monocytogenes in milk. Korean J. Food Sci. Anim. Resour., 37: 320-328. Ramirez, K; Cazarez-Montoya, C; Lopez-Moreno, and Castro-del Campo, N (2018). Bacteriophage cocktail for biocontrol of Escherichia coli O157: H7: Stability and potential allergenicity study. PLoS One. 13: e0195023.
Richards, PJ; Connerton, PL and Connerton, IF (2019). Phage biocontrol of Campylobacter jejuni in chickens does not produce collateral effects on the gut microbiota. Front. Microbiol., 10: 1-10.
Rouger, A; Tresse, O and Zagorec, M (2017). Bacterial contaminants of poultry meat: sources, species, and dynamics. Microorganisms. 5: 50-66.
Sonalika, J; Srujana, AS; Akhila, DS; Juliet, MR and Santhosh, KS (2020). Application of bacteriophages to control Salmonella Enteritidis in raw eggs. Iran. J. Vet. Res., 21: 221-225.
Sukumaran, AT; Nannapaneni, R; Kiess, A and Sharma, CS (2015). Reduction of Salmonella on chicken meat and chicken skin by combined or sequential application of lytic bacteriophage with chemical antimicrobials. Int. J. Food Microbiol., 207: 8-15.
Sukumaran, AT; Nannapaneni, R; Kiess, A and Sharma, GS (2016). Reduction of Salmonella on chicken breast fillets stored under aerobic or modified atmosphere packaging by the application of lytic bacteriophage preparation Salmo Fresh TM. Poult. Sci. J., 95: 668-675.
Titze, I; Lehnherr, T; Lehnherr, H and Kromker, V (2020). Efficacy of bacteriophages against Staphylococcus aureus isolates from bovine mastitis. Pharmaceuticals. 13: 35-57. | ||
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