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Comparative evaluation of various diagnostic techniques for detection of Cryptosporidium infection from the faecal samples of diarrhoeic bovine calves | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 247-254 اصل مقاله (545.69 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6818 | ||
| نویسندگان | ||
| V. Jaiswal* 1؛ A. P. S. Brar1؛ B. S. Sandhu1؛ L. D. Singla2؛ D. Narang3؛ G. D. Leishangthem1؛ P. Kaur4 | ||
| 1Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India | ||
| 2Department of Veterinary Parasitology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India | ||
| 3Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India | ||
| 4MSc in Biochemistry, Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India | ||
| چکیده | ||
| Background: Cryptosporidium, an opportunistic, zoonotic, apicomplexan parasite, is one of the most common causes of diarrhea in neonatal bovine calves around the globe. Bovine calves act as a major source of infection by excreting huge numbers of highly resistant oocysts in faeces, which can survive for a long time in extreme environmental conditions. As low as ten oocysts can cause disease and mortality, leading to the requirement of an early and accurate diagnosis for proper and favorable prognosis, management, and control. Aims: The current study was conducted with the objective to evaluate various diagnostic techniques (acid fast staining, negative staining, fluorescent, ELISA, PCR, nested PCR, and qPCR) for the detection of Cryptosporidium in the faecal samples of diarrheic bovine calves. Methods: Two hundred diarrheic faecal samples from bovine calves were collected and subjected to these techniques for Cryptosporidium diagnosis. Results of these were evaluated for diagnostic comparison. Results: Out of 200 faecal samples evaluated, 24% (48/200) were detected positive for Cryptosporidium using a combination of two techniques as gold standard criteria. Cohen’s kappa value indicated moderate to almost perfect agreement (0.616 to 0.986) among all the techniques used in the present study. Leishman staining showed the lowest sensitivity (54.17%), while nested PCR and qPCR showed the highest sensitivity (97.92%). Diagnostic specificity of all these tests ranged from 98.68 to 100%. Conclusion: Auramine stain was used for the first time in the bovine calves in India for the detection and diagnostic comparison of Cryptosporidium. It showed strong agreement with the molecular as well as classical diagnostic techniques, and can be used for primary screening for better diagnosis. | ||
| کلیدواژهها | ||
| Cryptosporidium؛ Diagnostic accuracy؛ ELISA؛ Fluorescence؛ PCR | ||
| مراجع | ||
|
Aghamolaie, S; Rostami, A; Fallahi, S; Tahvildar Biderouni, F; Haghighi, A and Salehi, N (2014). Evaluation of modified Ziehl-Neelsen, direct fluorescent-antibody and PCR assay for detection of Cryptosporidium spp. in children faecal specimens. J. Parasitol., 40: 958-963. https://doi.org/10.1007/s12639-014-0614-4.
Ahmed, SA and Karanis, P (2018). Comparison of current methods used to detect Cryptosporidium oocysts in stools. Int. J. Hyg. Environ. Health. 221: 743-763. https://doi.org/ 10.1016/j.ijheh.2018.04.006.
Bhat, SA; Dixit, M; Juyal, PD and Singh, NK (2014). Comparison of nested PCR and microscopy for the detection of cryptosporidiosis in bovine calves. J. Parasit. Dis., 38: 101-105. https://doi.org/10.1007/s12639-012-0201-5.
Blanchard, PC (2012). Diagnostics of dairy and beef cattle diarrhea. Vet. Clin. North. Am. Food Anim. Pract., 28: 443-464. https://doi.org/10.1016/j.cvfa.2012.07.002.
Brankston, G; Boughen, C; Ng, V; Fisman, DN; Sargeant, JM and Greer, AL (2018). Assessing the impact of environmental exposures and Cryptosporidium infection in cattle on human incidence of cryptosporidiosis in Southwestern Ontario, Canada. PLoS One. 13: e0196573. https://doi.org/10.1371/journal.pone.0196573.
Brar, A; Sood, NK; Kaur, P; Singla, LD; Sandhu, BS; Gupta, K; Narang, D; Singh, CK and Chandra, M (2017b). Periurban outbreaks of bovine calf scours in Northern India caused by Cryptosporidium in association with other enteropathogens. Epidemiol. Infect., 145: 2717-2726. https://doi.org/10.1017/S0950268817001224.
Brar, APS; Sood, NK; Singla, LD; Kaur, P; Gupta, K and Sandhu, BS (2017a). Validation of Romanowsky staining as a novel screening test for the detection of faecal cryptosporidial oocysts. J. Parasit. Dis., 41: 260-262. https://doi.org/10.1007/s12639-016-0788-z.
Chalmers, RM; Campbell, BM; Crouch, N; Charlett, A and Davies, AP (2011). Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assays used in the U.K. J. Med. Microbiol., 60: 1598-1604. https://doi.org/10. 1099/jmm.0.034181-0.
Danišová, O; Halánová, M; Valenčáková, A and Luptáková, L (2018). Sensitivity, specificity and comparison of three commercially available immunological tests in the diagnosis of Cryptosporidium species in animals. Braz. J. Microbiol., 49: 177-183. https://doi.org/ 10.1016/j.bjm.2017.03.016.
Das, K; Nair, LV; Ghosal, A; Sardar, SK; Dutta, S and Ganguly, S (2019). Genetic characterization reveals evidence for an association between water contamination and zoonotic transmission of a Cryptosporidium sp. from dairy cattle in West Bengal, India. Food. Waterborne Parasitol., 17: e00064. https://doi.org/10.1016/j.fawpar. 2019.e00064.
Díaz, P; Varcasia, A; Pipia, AP; Tamponi, C; Sanna, G; Prieto, A; Ruiu, A; Spissu, P; Díez-Baños, P; Morrondo, P and Scala, A (2018). Molecular characterisation and risk factor analysis of Cryptosporidium spp. in calves from Italy. Parasitol. Res., 117: 3081-3090. https://doi.org/10. 1007/s00436-018-6000-x.
Dzodanu, EG; Afrifa, J; Acheampong, DO and Dadzie, I (2019). Diagnostic yield of fluorescence and Ziehl-Neelsen staining techniques in the diagnosis of pulmonary tuberculosis: a comparative study in a district health facility. Tuberc. Res. Treat., 2019, 4091937. https://doi.org/ 10.1155/2019/4091937.
Elsafi, SH; Al-Sheban, SS; Al-Jubran, KM; Abu Hassan, MM and Al Zahrani, EM (2014). Comparison of Kinyoun’s acid-fast and immunofluorescent methods detected an unprecedented occurrence of Cryptosporidium in the Eastern Region of Saudi Arabia. J. Taibah. Univ. Sci., 9: 263-267. https://doi.org/10.1016/j.jtumed.2014.03. 008.
Feng, Y; Ortega, Y; He, G; Das, P; Xu, M; Zhang, X; Fayer, R; Gatei, W; Cama, V and Xiao, L (2007). Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Vet. Parasitol., 144: 1-9. https://doi.org/10.1016/j.vetpar.2006.10.001.
Ghaffari, S and Kalantari, N (2014). Recognition of Cryptosporidium oocysts in fresh and old stool samples: comparison of four techniques. Asian Pac. J. Trop. Biomed., 4: S570-S574. https://doi.org/10.12980/apjtb.4. 2014apjtb-2014-0067.
Ghoshal, U; Jain, V; Dey, A and Ranjan, P (2018). Evaluation of enzyme linked immunosorbent assay for stool antigen detection for the diagnosis of cryptosporidiosis among H.I.V. negative immuno-compromised patients in a tertiary care hospital of northern India. J. Infect. Public Health. 11: 115-119. https://doi.org/ 10.1016/j.jiph.2017.06.007.
Guy, RA; Yanta, CA; Muchaal, PK; Rankin, MA; Thivierge, K; Lau, R and Boggild, AK (2021). Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada. Parasit. Vectors. 14: 69. https://doi.org/ 10.1186/s13071-020-04546-9.
Hassan, EM; Örmeci, B; DeRosa, MC; Dixon, BR; Sattar, SA and Iqbal, A (2021). A review of Cryptosporidium spp. and their detection in water. Water Sci. Technol., 83: 1-25. https://doi.org/10.2166/wst.2020.515.
Henriksen, S and Pohlenz, J (1981). Staining of cryptosporidial by a modified Ziehl-Neelsen technique. Acta Vet. Scand., 22: 594-596.
Hingole, AC; Gudewar, JG; Pednekar, RP and Gatne, ML (2017). Prevalence and molecular characterization of Cryptosporidium species in cattle and buffalo calves in Mumbai region of India. J. Parasit. Dis., 41: 131-136. https://doi.org/10.1007/s12639-016-0763-8.
Izzo, M; Kirkland, P; Gu, X; Lele, Y; Gunn, A and House, J (2012). Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia. Aust. Vet. J., 90: 122-129. https://doi.org/10. 1111/j.1751-0813.2011.00891.x.
Jayalakshmi, J; Appalaraju, B and Mahadevan, K (2008). Evaluation of an enzyme-linked immunoassay for the detection of Cryptosporidium antigen in fecal specimens of HIV/AIDS patients. Indian. J. Pathol. Microbiol., 51: 137-138. https://doi.org/10.4103/0377-4929.40427.
Johnson, DW; Pieniazek, N; Griffin, DW; Misener, L and Rose, JB (995). Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol., 61: 3849-3855. https://doi.org/10.1128/aem.61.11.3849-3855.1995.
Joute, JR; Gill, J and Singh, BB (2016). Prevalence and molecular epidemiology of Cryptosporidium parvum in dairy calves in Punjab (India). J. Parasit. Dis., 40: 745-749. https://doi.org/10.1007/s12639-014-0571-y.
Kabir, M; Ceylan, O; Ceylan, C; Shehata, AA; Bando, H; Essa, MI; Xuan, X; Sevinc, F and Kato, K (2020). Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey. Parasitol. Int., 79: 102163. https://doi.org/10.1016/j.parint.2020.102163.
Kaushik, K; Khurana, S; Wanchu, A and Malla, N (2008). Evaluation of staining techniques, antigen detection and nested PCR for the diagnosis of cryptosporidiosis in HIV seropositive and seronegative patients. Acta Trop., 107: 1-7. https://doi.org/10.1016/j.actatropica.2008.02.007.
Khan, SM; Debnath, C; Pramanik, AK; Xiao, L; Nozaki, T and Ganguly, S (2010). Molecular characterization and assessment of zoonotic transmission of Cryptosporidium from dairy cattle in West Bengal, India. Vet. Parasitol., 171: 41-47. https://doi.org/10.1016/j.vetpar.2010.03.008.
Khurana, S; Sharma, P; Sharma, A and Malla, N (2012). Evaluation of Ziehl-Neelsen staining, auramine phenol staining, antigen detection enzyme linked immunosorbent assay and polymerase chain reaction, for the diagnosis of intestinal cryptosporidiosis. Trop. Parasitol., 2: 20-23. https://doi.org/10.4103/2229-5070.97234.
Kumar, A; Goyal, R; Kumar, A and Singh, P (2014). Comparative evaluation of microscopy and antigen detection for Cryptosporidium by ELISA in patients with immunocompromised status. IOSR-JDMS. 13: 4-8.
Mahmoudi, M; Tapeh, KH; Abasi, E; Sayyadi, H and Aminpour, A (2021). Prevalence and genetic characterization of Cryptosporidium in pre-weaned cattle in Urmia (Northwestern Iran). J. Infect. Dev. Ctries., 15: 422-427. https://doi.org/10.3855/jidc.12122.
Manouana, GP; Lorenz, E; Mbong, M; Nguema Moure, PA; Maiga Ascofaré, O; Akenten, CW; Amuasi, J; Rakotozandrindrainy, N; Rakotozandrindrainy, R; Mbwana, J; Lusingu, J; Byrne, N; Melhem, S; Zinsou, JF; Adegbite, RB; Hogan, B; Winter, D; May, J; Kremsner, PG; Borrmann, S; Eibach, D and Adegnika, AA (2020). Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. PLoS Negl. Trop. Dis., 14: e0008448. https://doi.org/10.1371/journal.pntd. 0008448.
Mayer, CL and Palmer, CJ (1996). Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater. Appl. Environ. Microbiol., 62: 2081-2085. https://doi.org/10. 1128/aem.62.6.2081-2085.1996.
McHugh, ML (2012). Interrater reliability: the kappa statistic. Biochemia. Medica. 22: 276-282.
Morgan, UM; Pallant, L; Dwyer, BW; Forbes, DA; Rich, G and Thompson, RC (1998). Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial. J. Clin. Microbiol., 36: 995-998. https://doi.org/10.1128/JCM.36.4.995-998. 1998.
Newman, RD; Jaeger, KL; Wuhib, T; Lima, AA; Guerrant, RL and Sears, CL (1993). Evaluation of an antigen capture enzyme-linked immunosorbent assay for detection of Cryptosporidium oocysts. J. Clin. Microbiol., 31: 2080-2084. https://doi.org/10.1128/jcm.31.8.2080-2084.1993.
Ninama, A (2018). Detection of Cryptosporidium in fecal sample by three staining method from immuno-compromised patients. Int. J. Med. Microbiol. Trop. Dis., 4: 257-260. https://doi.org/10.18231/2581-4761.2018.0055.
Ouakli, N; Belkhiri, A; de Lucio, A; Köster, PC; Djoudi, M; Dadda, A; Khelef, D; Kaidi, R and Carmena, D (2018). Cryptosporidium-associated diarrhoea in neonatal calves in Algeria. Vet. Parasitol. Reg. Stud. Reports. 12: 78-84. https://doi.org/10.1016/j.vprsr.2018.02.005.
Papini, R; Bonelli, F; Montagnani, M and Sgorbini, M (2018). Evaluation of three commercial rapid kits to detect Cryptosporidium parvum in diarrhoeic calf stool. Ital. J. Anim. Sci., 17: 1059-1064. https://doi.org/10.1080/ 1828051x.2018.1452055.
Paul, S; Chandra, D; Ray, DD; Tewari, AK; Rao, JR; Banerjee, PS; Baidya, S and Raina, OK (2008). Prevalence and molecular characterization of bovine Cryptosporidium isolates in India. Vet. Parasitol., 153: 143-146. https://doi.org/10.1016/j.vetpar.2008.01.044.
Paul, S; Chandra, D; Tewari, AK; Banerjee, PS; Ray, DD; Boral, R and Rao, JR (2009). Comparative evaluation and economic assessment of coprological diagnostic methods and PCR for detection of Cryptosporidium spp. in bovines. Vet. Parasitol., 164: 291-295. https://doi.org/10.1016/j. vetpar.2009.06.015.
Rekha, KM; Puttalakshmamma, GC and D’Souza, PE (2016). Comparison of different diagnostic techniques for the detection of cryptosporidiosis in bovines. Vet. World. 9: 211-215. https://doi.org/10.14202/vetworld.2016.211-215.
Ryan, U; Hijjawi, N and Xiao, L (2018). Foodborne cryptosporidiosis. Int. J. Parasitol., 48: 1-12. https://doi.org/ 10.1016/j.ijpara.2017.09.004.
Santin, M (2020). Cryptosporidium and Giardia in ruminants. Vet. Clin. North. Am. Food Anim. Pract., 36: 223-238. https://doi.org/10.1016/j.cvfa.2019.11.005.
Santín, M; Trout, JM; Xiao, L; Zhou, L; Greiner, E and Fayer, R (2004). Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet. Parasitol., 122: 103-117. doi: 10.1016/j.vetpar.2004. 03.020.
Shams, S; Abdul, Z; Khan, W and Campus, UCSS (2016). Differential techniques used for detection of Cryptosporidium oocysts in stool specimens. J. Parasit. Dis., 1: 1-11.
Shrivastava, AK; Kumar, S; Smith, WA and Sahu, PS (2017). Revisiting the global problem of cryptosporidiosis and recommendations. Trop. Parasitol., 7: 8-17. https://doi. org/10.4103/2229-5070.202290.
Singh, BB; Sharma, R; Kumar, H; Banga, HS; Aulakh, RS; Gill, JPS and Sharma, JK (2006). Prevalence of Cryptosporidium parvum infection in Punjab (India) and its association with diarrhea in neonatal dairy calves. Vet. Parasitol., 140: 162-165. https://doi.org/10.1016/j.vetpar. 2006.03.029.
Tanriverdi, S; Tanyeli, A; Başlamişli, F; Köksal, F; Kilinç, Y; Feng, X; Batzer, G; Tzipori, S and Widmer, G (2002). Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis. J. Clin. Microbiol., 40: 3237-3244. https://doi.org/10.1128/JCM.40.9.3237-3244.2002.
Thomson, S; Hamilton, CA; Hope, JC; Katzer, F; Mabbott, NA; Morrison, LJ and Innes, EA (2017). Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Vet. Res., 48: 42. https://doi.org/10. 1186/s13567-017-0447-0. Toledo, RDS; Martins, FDC and Freire, RL (2017). Waterborne Giardia and Cryptosporidium: contamination of human drinking water by sewage and cattle feces. Semin. Agrar., 38: 3395-3415. https://doi.org/10.5433/ 1679-0359.2017v38n5p3395.
Trotz-Williams, LA; Jarvie, BD; Martin, SW; Leslie, KE and Peregrine, AS (2005). Prevalence of Cryptosporidium parvum infection in southwestern Ontario and its association with diarrhea in neonatal dairy calves. Can. Vet. J., 46: 349-351.
Vejdani, M; Mansour, R; Hamzavi, Y; Vejdani, S; Nazeri, N and Michaeli, A (2014). Immunofluorescence assay and PCR analysis of Cryptosporidium oocysts and species from human fecal specimens. Jundishapur J. Microbiol., 7: e10284. https://doi.org/10.5812/jjm.10284.
Venu, R; Latha, BR; Basith, SA; Raj, GD; Sreekumar, C and Raman, M (2012). Molecular prevalence of Cryptosporidium spp. in dairy calves in Southern states of India. Vet. Parasitol., 188: 19-24. https://doi.org/10.1016/j. vetpar.2012.02.025.
Vermeulen, LC; Benders, J; Medema, G and Hofstra, N (2017). Global Cryptosporidium loads from livestock manure. Environ. Sci. Technol., 51: 8663-8671. https://doi.org/10.1021/acs.est.7b00452.
Vohra, P; Sharma, M and Chaudhary, U (2012). A comprehensive review of diagnostic techniques for detection of Cryptosporidium parvum in stool samples. IOSR. J. Pharm., 2: 15-26.
Wang, R; Zhao, G; Gong, Y and Zhang, L (2017). Advances and perspectives on the epidemiology of bovine Cryptosporidium in China in the past 30 years. Front. Microbiol., 8: 1823. https://doi.org/10.3389/fmicb.2017. 01823.
Wells, B; Paton, C; Bacchetti, R; Shaw, H; Stewart, W; Plowman, J; Katzer, F and Innes, EA (2019). Cryptosporidium prevalence in calves and geese co-grazing on four livestock farms surrounding two reservoirs supplying public water to Mainland Orkney, Scotland. Microorganisms. 7: 513. https://doi.org/10.3390/ microorganisms7110513.
Xiao, L (2010). Molecular epidemiology of cryptosporidiosis: an update. Exp. Parasitol., 124: 80-89. https://doi.org/10. 1016/j.exppara.2009.03.018.
Xiao, L; Fayer, RL; Ryan, UL and Upton, SJ (2004). Cryptosporidium taxonomy: recent advances and implications for public health. Clin. Microbiol. Rev., 17: 72-97. https://doi.org/10.1128/cmr.17.1.72-97.2004.
Yildirim, A; Adanir, R; Inci, A; Yukari, BA; Duzlu, O; Onder, Z; Ondera, Z; Ciloglua, A and Simsek, E (2020). Prevalence and genotyping of bovine Cryptosporidium species in the Mediterranean and Central Anatolia Region of Turkey. Comp. Immunol. Microbiol. Infect. Dis., 69: 101425. https://doi.org/10.1016/j.cimid.2020.101425.
Yimer, M; Gezhagne, M; Biruk, T and Dinaol, B (2015). A review on major bacterial causes of calf diarrhea and its diagnostic method. J. Vet. Med. Anim. Health. 7: 173-185. https://doi.org/10.5897/JVMAH2014.0351.
Yu, JR; Lee, SU and Park, WY (2009). Comparative sensitivity of PCR primer sets for detection of Cryptosporidium parvum. Korean J. Parasitol., 47: 293-297. https://doi.org/10.3347/kjp.2009.47.3.293. | ||
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