| تعداد نشریات | 24 |
| تعداد شمارهها | 912 |
| تعداد مقالات | 8,158 |
| تعداد مشاهده مقاله | 15,836,153 |
| تعداد دریافت فایل اصل مقاله | 13,332,060 |
Expression of α- and ε-toxin genes in Clostridium perfringens type D vaccine strain in contact with the Caco-2 cell line | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 229-236 اصل مقاله (529.5 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6816 | ||
| نویسندگان | ||
| A. R. Forouzan1؛ N. Moori Bakhtiari* 2؛ M. R. Seify abad Shapouri2؛ F. Salabi3؛ M. Khosravi2 | ||
| 1Ph.D. Student in Veterinary Bacteriology, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Department of Venomous Animals and Anti-Venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran | ||
| چکیده | ||
| Background: Clostridium perfringens commonly resides in the gastrointestinal tract and can survive in different environmental conditions. This pathogen produces several protein toxins including the potent ε-toxin which is classified as a category B toxin by the Centers for Disease Control and Prevention (CDC). In several studies, the induction of C. perfringens type C or D to produce toxins much more rapidly by close contact of bacteria with Caco-2 cells has been reported. Aims: The effect of close contact of enterocyte-like Caco-2 cells with C. perfringens type D (vaccine strain) on the production time of ε- and α-toxins was studied. Methods: During C. perfringens type D contact with Caco-2 cells for 5 h, ε- and α-toxins expressions (at 0, 2, and 5 h) were evaluated by a quantitative real-time PCR assay. Non-contacted bacteria with cells were included as the negative control in this research. Results: Bacterial contact with the Caco-2 cells induces a significant effect on the mean expression of the ε-toxin gene (etx) (P<0.05). Two h after contact, the highest level of gene expression was detected in the experimental group. Bacterial harvesting time, cell treatment, and their interactions did not affect significantly the mean expression of the α-toxin gene (cpa) (P>0.05). Conclusion: According to the findings of the present study, 2 h of bacterial contact with Caco-2 cells could stimulate etx gene expression in the C. perfringens type D vaccine strain. | ||
| کلیدواژهها | ||
| Caco-2 cells؛ Clostridium perfringens؛ Gene expression؛ Toxin | ||
| مراجع | ||
|
Amimoto, K; Noro, T; Oishi, E and Shimizu, M (2007). A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Microbiology. 153: 1198-1206. https://doi: 10.1099/mic.0.2006/002287-0.
Chen, J; Ma, M; Uzal, FA and McClane, BA (2014). Host cell-induced signaling causes Clostridium perfringens to upregulate production of toxins important for intestinal infections. Gut Microbes. 5: 96-107. https://doi: 10.4161/ gmic.26419.
Chen, J; Rood, JI and McClane, BA (2011). Epsilon-toxin production by Clostridium perfringens type D strain CN3718 is dependent upon the agr operon but not the VirS/VirR two-component regulatory system. MBio. 2: e00275-11. https://doi.org/10.1128/mBio.00275-11.
Fisher, DJ; Fernandez-Miyakawa, ME; Sayeed, S; Poon, R; Adams, V; Rood, JI; Uzal, FA and McClane, BA (2006). Dissecting the contributions of Clostridium perfringens type C toxins to lethality in the mouse intravenous injection model. Infect. Immun., 74: 5200-5210.
Florence, LCH; Hakim, SL; Kamaluddin, MA and Thong, KL (2011). Determination of toxinotypes of environmental Clostridium perfringens by Polymerase Chain Reaction. Trop. Biomed., 28: 171-174.
Gkiourtzidis, K; Frey, J; Bourtzi-Hatzopoulou, E; Iliadis, N and Sarris, K (2001). PCR detection and prevalence of α-, β-, β2-, ε-, ι- and enterotoxin genes in Clostridium perfringens isolated from lambs with clostridial dysentery. Vet. Microbiol., 82: 39-43.
Gray, B; Hall, P and Gresham, H (2013). Targeting agr- and agr-like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections. Sensors. 13: 5130-5166.
Li, J; Sayeed, S; Robertson, S; Chen, J and McClane, BA (2011). Sialidases affect the host cell adherence and epsilon toxin-induced cytotoxicity of Clostridium perfringens type D strain CN3718. PLoS Pathog., 7: e1002429. https://doi: 10.1371/journal.ppat.1002429.
Matsuki, T; Watanabe, K; Fujimoto, J; Takada, T and Tanaka, R (2004). Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl. Environ. Microbiol., 70: 7220-7228. https://doi: 10.1128/AEM.70.12.7220-7228. 2004.
Ohtani, K (2016). Gene regulation by the VirS/VirR system in Clostridium perfringens. Anaerobe. 41: 5-9. https://doi.org/ 10.1016/j.anaerobe.2016.06.003.
Ohtani, K and Shimizu, T (2016). Regulation of toxin production in Clostridium perfringens. Toxins, (Basel). 8: 207-221. https://doi.org/10.3390/toxins8070207.
Petit, L; Gibert, M; Gourch, A; Bens, M; Vandewalle, A and Popoff, MR (2003). Clostridium perfringens epsilon toxin rapidly decreases membrane barrier permeability of polarized MDCK cells. Cell Microbiol., 5: 155-164. https://doi: 10.1128/jb.179.20.6480-6487.1997.
Pfaffl, MW; Horgan, GW and Dempfle, L (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res., 30: e36. https://doi: 10.1093/nar/30.9.e36.
Poormontaseri, M; Hosseinzadeh, S; Shekarforoush, SS and Kalantari, T (2017). The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type A in Caco-2 cell culture. BMC Microbiol., 17: 1-8. https://doi: 10.1186/s12866-017-1051-1.
Popoff, MR (2011). Epsilon toxin: a fascinating pore-forming toxin. FEBS. 278: 4602-4615. https//doi: 10.1111/j.1742-4658.2011.08145.x.
Revitt-Mills, SA; Rood, JI and Adams, V (2015). Clostridium perfringens extracellular toxins and enzymes: 20 and counting. Microbiol. Aust., 36: 114-117.
Rood, JI; Adams, V; Lacey, J; Lyras, D; McClane, BA; Melville, SB; Moore, RJ; Popoff, MR; Sarker, MR; Songer, JG; Uzal, FA and Immerseel, FV (2018). Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe. 53: 5-10. doi: 10.1016/j. anaerobe.2018.04.011.
Schlegel, BJ; Nowell, VJ; Parreira, VR; Soltes, G and Prescott, JF (2012). Toxin-associated and other genes in Clostridium perfringens type A isolates from bovine clostridial abomasitis (BCA) and jejunal hemorrhage syndrome (JHS). Can. J. Vet. Res., 76: 248-254.
Shimamoto, S; Tamai, E; Matsushita, O; Minami, J; Okabe, A and Miyata, S (2005). Changes in ganglioside content affect the binding of Clostridium perfringens epsilon-toxin to detergent-resistant membranes of Madin-Darby canine kidney cells. Microbiol. Immunol., 49: 245-253.
Shimizu, T; Ohtani, K; Hirakawa, H; Ohshima, K; Yamashita, A; Shiba, T; Ogasawara, N; Hattori, M; Kuhara, S and Hayashi, H (2002). Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. PNAS. 99: 996-1001.
Uzal, FA; Freedman, JC; Shrestha, A; Theoret, JR; Garcia, J; Awad, MM; Adams, V; Moore, RJ; Rood, JI and McClane, BA (2014). Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease. Future Microbiol., 9: 361-377.
Uzal, FA; Kelly, WR; Morris, WE; Bermudez, J and Baisón, M (2004). The pathology of peracute experimental Clostridium perfringens type D enterotoxemia in sheep. J. Vet. Diagn. Invest., 16: 403-411. Vidal, JE; Ohtani, K; Shimizu, T and McClane, BA (2009). Contact with enterocyte-like Caco-2 cells induces rapid upregulation of toxin production by Clostridium perfringens type C isolates. Cell Microbiol., 11: 1306-1328. https//doi: 10.1111/j.1462-5822.2009.01332.x.
Wang, H; Shen, X; Zhao, Y; Wang, M; Zhong, Q; Chen, T; Hu, F and Li, M (2012). Identification and proteome analysis of the two-component VirR/VirS system in epidemic Streptococcus suis serotype 2. FEMS Microbiol. Lett., 333: 160-168. doi: 10.1111/j.1574-6968.2012.02611. x. | ||
|
آمار تعداد مشاهده مقاله: 518 تعداد دریافت فایل اصل مقاله: 392 |
||