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Valizadeh, Sepehr, Zehtabi, Mojtaba, Feiziordaklou, Neda, Akbarpour, Zahra, Khamaneh, Amir Mahdi, Raeisi, Mortaza. (1401). Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. , 11(3), 133-141. doi: 10.22099/mbrc.2022.43947.1757
Sepehr Valizadeh; Mojtaba Zehtabi; Neda Feiziordaklou; Zahra Akbarpour; Amir Mahdi Khamaneh; Mortaza Raeisi. "Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis". , 11, 3, 1401, 133-141. doi: 10.22099/mbrc.2022.43947.1757
Valizadeh, Sepehr, Zehtabi, Mojtaba, Feiziordaklou, Neda, Akbarpour, Zahra, Khamaneh, Amir Mahdi, Raeisi, Mortaza. (1401). 'Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis', , 11(3), pp. 133-141. doi: 10.22099/mbrc.2022.43947.1757
Valizadeh, Sepehr, Zehtabi, Mojtaba, Feiziordaklou, Neda, Akbarpour, Zahra, Khamaneh, Amir Mahdi, Raeisi, Mortaza. Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. , 1401; 11(3): 133-141. doi: 10.22099/mbrc.2022.43947.1757

Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis

Molecular Biology Research Communications
دوره 11، شماره 3، 2022، صفحه 133-141    اصل مقاله (217.33 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.43947.1757
نویسندگان
Sepehr Valizadeh1؛ Mojtaba Zehtabi2؛ Neda Feiziordaklou1؛ Zahra Akbarpour3؛ Amir Mahdi Khamaneh4؛ Mortaza Raeisi* 2
1Department of Internal Medicine, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Rahat Breathe and Sleep Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.
کلیدواژه‌ها
Papillary thyroid cancer؛ MicroRNA؛ Bioinformatics؛ Microarray data analysis
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مراجع
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