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Phylogenetic relationship, virulence factors, and biofilm formation ability of human, pet animals, and raw milk Staphylococcus aureus isolates | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 181-188 اصل مقاله (721.85 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6760 | ||
| نویسندگان | ||
| S. Pahlavanzadeh1؛ R. Khoshbakht* 2؛ H. Kaboosi3؛ E. Moazamian4 | ||
| 1Ph.D. Student in Microbiology, Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran | ||
| 3Department of Microbiology, Faculty of Basic Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran | ||
| 4Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| چکیده | ||
| Background: Identification of genotypic characteristics and pathogenicity of Staphylococcus aureus isolates is very important in the epidemiological study of its related diseases. Aims: The present study was done to compare the S. aureus isolates from different sources on the basis of virulence gene properties, biofilm production ability, and phylogenetic variations. Methods: Seventy S. aureus isolates (including 25 human, 25 raw milk, and 20 pet animal isolates) were subjected to slime production ability testing, polymerase chain reaction (PCR) detection of 14 different virulence genes, and DNA fingerprinting using restriction fragment length polymorphism (RFLP) of coa gene PCR products. Results: Among 70 S. aureus, 64 (91.4%) isolates were slime producers on Congo red agar (CRA) medium. The spa and icaD virulence genes were present in all isolates and the seD and etaA genes were not detected in any of the isolates. In total, 22 different virulence gene patterns and nine distinct clusters of coa-PCR-RFLP were identified among isolates. Conclusion: According to the results, S. aureus strains of human origin showed a significant association with specific virulence gene profiles and genotypes. seB and seC were the most responsible genes for S. aureus enterotoxin among human and animal isolates, respectively. Coa-RFLP showed partially appropriate results in the classification and source detection of S. aureus isolates. | ||
| کلیدواژهها | ||
| Biofilm؛ PCR-RFLP؛ Staphylococcus aureus؛ Virulence factors | ||
| مراجع | ||
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Aalaa’A, C and Abd Al-Abbas, MJ )2019(. PCR-RFLP by AluI for coa gene of methicillin-resistant Staphylococcus aureus (MRSA) isolated from burn wounds, pneumonia and otitis media. Gene Rep., 15: 100390.
Aires-de-Sousa, M (2017). Methicillin-resistant Staphylo-coccus aureus among animals: current overview. Clin. Microbiol. Infect., 23: 373-380.
Al-Mebairik, NF; El-Kersh, TA; Al-Sheikh, YA and Marie, MAM (2016). A review of virulence factors, pathogenesis, and antibiotic resistance in Staphylococcus aureus. Rev. Med. Microbiol., 27: 50-56.
Arciola, CR; Baldassarri, L and Montanaro, L (2001). Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. J. Clin. Microbiol., 39: 2151-2156.
Arciola, CR; Campoccia, D; Gamberini, S; Cervellati, M; Donati, E and Montanaro, L (2002). Detection of slime production by means of an optimised Congo red agar plate test based on a colourimetric scale in Staphylococcus epidermidis clinical isolates genotyped for ica locus. Biomaterials. 23: 4233-4239.
Cheraghi, S; Pourgholi, L; Shafaati, M; Fesharaki, SH; Jalali, A; Nosrati, R and Boroumand, MA (2017). Analysis of virulence genes and accessory gene regulator (agr) types among methicillin-resistant Staphylococcus aureus strains in Iran. J. Glob. Antimicrob. Resist., 10: 315-320.
Cheung, GY; Bae, JS and Otto, M (2021). Pathogenicity and virulence of Staphylococcus aureus. Virulence. 12: 547-569.
Ciftci, A; Findik, A; Onuk, EE and Savasan, S (2009). Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis. Braz. J. Microbiol., 40: 254-261.
Dendani, ZC; Bezille, P and Arcangioli, MA (2016). PCR and PCR-RFLP genotyping of Staphylococcus aureus coagulase gene: convenience compared to pulse-field gel electrophoresis. Comp. Clin. Path., 25: 1061-1064.
Freeman, DJ; Falkiner, FR and Keane, CT (1989). New method for detecting slime production by coagulase negative staphylococci. J. Clin. Path., 42: 872-874.
Goh, SH; Byrne, SK; Zhang, JL and Chow, AW (1992). Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J. Clin. Microbiol., 30: 1642-1645.
Hakimi Alni, R; Mohammadzadeh, A; Mahmoodi, P and Alikhani, MY (2017). Genotypic analysis of Staphylo-coccus aureus coagulase gene using PCR-RFLP analysis. Med. Lab. J., 11: 12-17.
Halim, RMA; Kassem, NN and Mahmoud, BS (2018). Detection of biofilm producing staphylococci among different clinical isolates and its relation to methicillin susceptibility. Open Access. Maced. J. Med. Sci., 6: 1335-1341.
Jarraud, S; Mougel, C; Thioulouse, J; Lina, G; Meugnier, H; Forey, F; Nesme, X; Etienne, J and Vandenesch, F (2002). Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect. Immun., 70: 631-641.
Lina, G; Piémont, Y; Godail-Gamot, F; Bes, M; Peter, MO; Gauduchon, V; Vandenesch, F and Etienne, J (1999). Involvement of Panton-Valentine leukocidin—producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis., 29: 1128-1132.
Magro, G; Biffani, S; Minozzi, G; Ehricht, R; Monecke, S; Luini, M and Piccinini, R (2017). Virulence genes of S. aureus from dairy cow mastitis and contagiousness risk. Toxins. 9: 195.
Mahmoudi, H; Arabestani, MR; Mousavi, SF and Alikhani, MY (2017). Molecular analysis of the coagulase gene in clinical and nasal carrier isolates of methicillin-resistant Staphylococcus aureus by restriction fragment length polymorphism. J. Glob. Antimicrob. Resist., 8: 41-45.
Mehrotra, M; Wang, G and Johnson, WM (2000). Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbial., 38: 1032-1035.
Mohajeri, P; Azizkhani, S; Farahani, A and Norozi, B (2016). Genotyping of coa and aroA genes of methicillin-resistant Staphylococcus aureus strains isolated from nasal samples in western Iran. Jundishapur J. Microbiol., 9: e26460.
Pahlavanzadeh, S; Khoshbakht, R; Kaboosi, H and Moazamian, E (2021). Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates. Comp. Immunol. Microbiol. Infect. Dis., 79: 101717. Savariraj, WR; Ravindran, NB; Kannan, P; Paramasivam, R; Senthilkumar, TMA; Kumarasamy, P and Rao, VA (2019). Prevalence, antimicrobial susceptibility and virulence genes of Staphylococcus aureus isolated from pork meat in retail outlets in India. J. Food Saf., 39: e12589.
Sohail, M and Latif, Z (2018). Molecular typing of methicillin resistance Staphylococcus aureus (MRSA) isolated from device related infections by SCCmec and PCR-RFLP of coagulase gene. Adv. Life Sci., 6: 34-40.
Taj, Y; Essa, F; Aziz, F and Kazmi, SU (2012). Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. J. Infect. Dev. Ctries., 6: 403-409.
Tiwari, HK; Sapkota, D; Gaur, A; Mathuria, JP; Singh, A and Sen, MR (2008). Molecular typing of clinical Staphylococcus aureus isolates from northern India using coagulase gene PCR-RFLP. Southeast Asian J. Trop. Med. Public Health. 39: 467-473.
Vasudevan, P; Nair, MKM; Annamalai, T and Venkitanarayanan, KS (2003). Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylo-coccus aureus for biofilm formation. Vet. Microbiol., 92: 179-185.
Wada, M; Lkhagvadorj, E; Bian, L; Wang, C; Chiba, Y; Nagata, S; Shimizu, T; Yamashiro, Y; Asahara, T and Nomoto, K (2010). Quantitative reverse transcription-PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus. J. Appl. Microbiol., 108: 779-788.
Zimmerli, W; Trampuz, A and Ochsner, PE (2004). Prosthetic-joint infections. New Engl. J. Med., 351: 1645-1654.
Zmantar, T; Chaieb, K; Makni, H; Miladi, H; Abdallah, FB; Mahdouani, K and Bakhrouf, A (2008). Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus. J. Basic Microbiol., 48: 308-314. | ||
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