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Heidary, Fatemeh, Tourani, Mehdi, Hejazi-Amiri, Fatemeh, Khatami, Seyyed Hossein, Jamali, Navid, Taheri-Anganeh, Mortaza. (1400). Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study. , 11(1), 55-66. doi: 10.22099/mbrc.2022.42468.1697
Fatemeh Heidary; Mehdi Tourani; Fatemeh Hejazi-Amiri; Seyyed Hossein Khatami; Navid Jamali; Mortaza Taheri-Anganeh. "Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study". , 11, 1, 1400, 55-66. doi: 10.22099/mbrc.2022.42468.1697
Heidary, Fatemeh, Tourani, Mehdi, Hejazi-Amiri, Fatemeh, Khatami, Seyyed Hossein, Jamali, Navid, Taheri-Anganeh, Mortaza. (1400). 'Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study', , 11(1), pp. 55-66. doi: 10.22099/mbrc.2022.42468.1697
Heidary, Fatemeh, Tourani, Mehdi, Hejazi-Amiri, Fatemeh, Khatami, Seyyed Hossein, Jamali, Navid, Taheri-Anganeh, Mortaza. Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study. , 1400; 11(1): 55-66. doi: 10.22099/mbrc.2022.42468.1697

Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study

Molecular Biology Research Communications
دوره 11، شماره 1، 2022، صفحه 55-66    اصل مقاله (544.07 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.42468.1697
نویسندگان
Fatemeh Heidary1؛ Mehdi Tourani2؛ Fatemeh Hejazi-Amiri3؛ Seyyed Hossein Khatami4؛ Navid Jamali5؛ Mortaza Taheri-Anganeh* 6
1Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
3Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
4Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Department of Laboratory Sciences, Sirjan School of Medical Sciences, Sirjan, Iran
6Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran
چکیده
Lung cancer is the most common type of tumor worldwide. Non-small-cell lung carcinoma (NSCLC) is considered any epithelial cell-related lung cancer, which includes more than 85% of all lung cancer cases. NSCLC is less responsive to chemotherapy than SCLC. Therefore, the need for other treatments has become more pronounced and immunotherapy has gained increasing attention as a promising therapy in recent years. The current study aimed to design a multi-epitope peptide vaccine targeting main cancer/testis antigens of SP17, AKAP4, and PTTG1, which have a major function in tumor cell proliferation invasion. The protein vaccine was constructed using the rigorous immunoinformatics analysis and investigation of several immune system parameters, considering B cell epitopes and CD4 and CD8 induced epitopes as the most important cells to respond to cancer cells. Inverse translation and optimization of codons were performed to have the designed protein's cloning as well as expression potential in E.coli. Physicochemical, antigenic, and allergenic features were assessed to confirm the safety and immunogenicity of the vaccine. The secondary and tertiary structures were predicted. Finally, intrinsic disorder and 3D model refinement and validation were performed to eliminate structural problems. The designed construct had a stable structure that could be an antigen and stimulate the immune system and not be an allergen. The built model 3D structure was valid and stable. Further investigations are needed to approve the safety and immunogenic property of this new vaccine for NSCLC before it can be used in patients.
کلیدواژه‌ها
Lung cancer؛ Bioinformatics؛ Epitope Vaccine
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