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Khorsand, Marjan, Mostafavi-Pour, Zohreh, Razban, Vahid, Khajeh, Sahar, Zare, Razieh. (1400). Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells. , 11(1), 11-20. doi: 10.22099/mbrc.2022.42638.1700
Marjan Khorsand; Zohreh Mostafavi-Pour; Vahid Razban; Sahar Khajeh; Razieh Zare. "Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells". , 11, 1, 1400, 11-20. doi: 10.22099/mbrc.2022.42638.1700
Khorsand, Marjan, Mostafavi-Pour, Zohreh, Razban, Vahid, Khajeh, Sahar, Zare, Razieh. (1400). 'Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells', , 11(1), pp. 11-20. doi: 10.22099/mbrc.2022.42638.1700
Khorsand, Marjan, Mostafavi-Pour, Zohreh, Razban, Vahid, Khajeh, Sahar, Zare, Razieh. Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells. , 1400; 11(1): 11-20. doi: 10.22099/mbrc.2022.42638.1700

Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells

Molecular Biology Research Communications
دوره 11، شماره 1، 2022، صفحه 11-20    اصل مقاله (796.34 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.42638.1700
نویسندگان
Marjan Khorsand1، 2؛ Zohreh Mostafavi-Pour* 1، 3؛ Vahid Razban4، 5؛ Sahar Khajeh6؛ Razieh Zare1
1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
3Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4Molecular Medicine Department, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
5Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
6Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
The epithelial-to-mesenchymal transition (EMT) is a unique process resulting in enhanced cell motility, invasiveness, and metastasis in cancer. The EMT is regulated by several transcription factors, including Snail and Slug, which exert crucial roles during cancer progression. We have studied the effects of Docetaxel as the first-line chemotherapy agent for prostate cancer, and Telmisartan as an anti-hypertensive drug on the expression level of Snail and Slug. In addition, the effects of Docetaxel, Telmisartan and their combination on cancer cell proliferation were investigated. The PC3, DU145, MDA-MB468, and HEK cell lines were used for this study. Quantitative RT-PCR analysis and MTT assay were used to study the expression of Snail and Slug level and cell proliferative assay, respectively. We found that a combination of Docetaxel + Telmisartan effectively inhibits the cell proliferation in cancerous cells in comparison with each drug alone (P<0.05). Furthermore, in these cell lines, Docetaxel, Telmisartan and their combination significantly diminished the expression level of Snail and Slug genes compared to control cells (P<0.001), however, in the HEK cell line, this effect was seen only in the combination group. Our data imply that Telmisartan and its combination with Docetaxel exert strong inhibitory effects on the expression level of Snail and Slug genes. Also, these drugs and their combination could inhibit cancer cell proliferation. In conclusion, the combination of Telmisartan and Docetaxel has the potential to suppress the metastasis of prostate and breast cancer cells.
کلیدواژه‌ها
Cancer؛ Combination index؛ EMT؛ Slug؛ Snail
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