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Virulence, MLST analysis, and antimicrobial resistance of Campylobacter coli isolated from broiler chickens in Tamil Nadu, India | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 2 - شماره پیاپی 79، 2022، صفحه 128-136 اصل مقاله (729.31 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.42199.6135 | ||
| نویسندگان | ||
| K. Gunasekaran1؛ S. Vellapandi2؛ M. Ananda Chitra* 3؛ K. Kumaragurubaran3 | ||
| 1MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai-600007, Tamil Nadu, India | ||
| 2MSc Student in Biotechnology, Department of Biotechnology, University of Madras, Chennai-600025, Tamil Nadu, India | ||
| 3Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India | ||
| چکیده | ||
| Background: Campylobacter species are the zoonotic bacteria and the most common cause of foodborne gastroenteritis around the world. The link between human campylobacteriosis and infected poultry consumption has been well established. Aims: In this study, we aimed to isolate Campylobacter spp. from chicken and characterize them with molecular methods. Methods: Totally, 241 chicken caecal mucosal scrapings were collected from five districts of Tamil Nadu. Bacterial isolation was done by plating on blood-free Campylobacter selective medium with supplements. Campylobacter species were identified by multiplex PCR and Campylobacter coli isolates were tested for 11 virulence genes by PCR. C. coli isolates were typed based on seven housekeeping genes multilocus sequence typing (MLST) scheme. The antimicrobial susceptibility was determined by a microdilution resazurin assay. Results: The prevalence of C. coli and C. jejuni were 14.94% (36/241), and 3.32% (8/241), respectively. The virulence genes flaA, flaB, cadF, cdtA, cdtB, cdtC, ciaB, and ceuE were present in all 36 C. coli isolates, pldA and racR genes were present in 58.33% (21/36), and 16.67% (6/36) of the isolates, respectively, and dnaJ was present in only one isolate. Two novel sequence types (ST-10872, ST-11031) were found in this study. Though different STs were identified, all the STs belonged to the same clonal complex of ST-828. All 14 C. coli isolates showed 100% resistance to nalidixic acid, and higher resistance to tetracycline (92.8%), erythromycin (71.4%), clindamycin (71.4%), and azithromycin (64.2%) was noticed. All C. coli isolates were sensitive to chloramphenicol, and higher sensitivity to ciprofloxacin (78.5%), and gentamicin (71.4%) was observed. Conclusion: The present study demonstrated that C. coli is more prevalent in broilers than C. jejuni in Tamil Nadu. The presence of C. jejuni and C. coli in chicken caecal samples from the slaughterhouse are indicative of the possibility of public health hazards. | ||
| کلیدواژهها | ||
| Antimicrobial resistance؛ Campylobacter coli؛ Multilocus sequence typing؛ Multiplex PCR؛ Virulence genes | ||
| مراجع | ||
|
Ananda Chitra, M; Jayanthy, C and Nagarajan, B (2015). Detection and sequence analysis of accessory gene regulator genes of Staphylococcus pseudintermedius isolates. Vet. World. 8: 902-907. http://dx.doi.org/10. 14202/vetworld.902-907.
Bachoual, R; Ouabdesselam, S; Mory, F; Lascols, C; Soussy, CJ and Tankovic, J (2001). Single or double mutational alterations of gyrA associated with fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli. Microb. Drug Resist., 7: 257-261. https://doi.org/10.1089/10766290152652800.
Bolton, DJ (2015). Campylobacter virulence and survival factors. Food Microbiol., 48: 99-108. https://doi.org/10. 1016/j.fm.2014.11.017.
Byrd, JA; Hargis, BM; Caldwell, DJ; Bailey, RH; Herron, KL; McReynolds, JL; Brewer, RL; Anderson, RC; Bischoff, KM and Callaway, TR (2001). Effect of lactic acid administration in the drinking water during preslaughter feed withdrawal on Salmonella and Campylobacter contamination of broilers. Poult. Sci., 80: 278-283. https://doi.org/10.1093/ps/80.3.278.
Caffrey, N; Agunos, A; Gow, S; Liljebjelke, K; Waldner, CL; Mainali, C and Checkley, SL (2021). A cross-sectional study of the prevalence factors associated with fluoroquinolone resistant Campylobacter jejuni in broiler flocks in Canada. Prev. Vet. Med., 186: 105-164. http://dx.doi.org/10.1016/j.prevetmed.2020.105164.
CLSI (2020). Performance standards for antimicrobial susceptibility testing CLSI supplement M100. (30th Edn.), Clinical and Laboratory Standards Institute, Wayne, PA.
Datta, S; Niwa, H and Itoh, K (2003). Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. J. Med. Microbiol., 52: 345-348.
Denis, M; Nagard, B; Rose, V; Bourgoin, K; Cutimbo, M and Kerouanton, A (2017). No clear differences between organic or conventional pig farms in the genetic diversity or virulence of Campylobacter coli isolates. Front. Microbiol., 8: 1016.
Dingle, KE; Colles, FM; Wareing, DRA; Ure, R; Fox, AJ; Bolton, FE; Bootsma, HJ; Willems, RJL; Urwin, R and Maiden, MCJ (2001). Multilocus sequence typing system for Campylobacter jejuni. J. Clin. Microbiol., 39: 14-23.
Engberg, J; Aarestrup, FM; Taylor, DE; Gerner-Smidt, P and Nachamkin, I (2001). Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg. Infect. Dis., 7: 24-34. doi: 10.3201/eid0701.010104.
Gahamanyi, N; Song, DG; Yoon, KY; Mboera, LE; Matee, MI; Mutangana, D; Amachawadi, RG; Komba, EV and Pan, CH (2021). Antimicrobial resistance profiles, virulence genes, and genetic diversity of thermophilic Campylobacter species isolated from a layer poultry farm in Korea. Front. Microbiol., 12: 554. https://doi.org/10. 3389/fmicb.2021.622275.
García-Sánchez, L; Melero, B; Diez, AM; Jaime, I; Canepa, A and Rovira, J (2020). Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp. isolated during two seasonal periods in Spanish poultry farms. Prev. Vet. Med., 176: 104935. https://doi.org/10.1016/j. prevetmed.2020.104935.
Gonzalez, I; Grant, KA; Richardson, PT; Park, SF and Collins, MD (1997). Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant. J. Clin. Microbiol., 35: 759-763.
Goon, S; Kelly, JF; Logan, SM; Ewing, CP and Guerry, P (2003). Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene. Mol. Microbiol., 50: 659-671.
Jesse, TW; Englen, MD; Pittenger-Alley, LG and Fedorka-Cray, PJ (2006). Two distinct mutations in gyrA lead to ciprofloxacin and nalidixic acid resistance in Campylobacter coli and Campylobacter jejuni isolated from chickens and beef cattle. J. Appl. Microbiol., 100: 682-688. https://doi.org/10.1111/j.1365-2672.2005.02796.
Jolley, KA; Bray, JE and Maiden, MCJ (2018). Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications. Wellcome Open Res., 3: 124. doi: 10.12688/wellcomeopenres.14826. 1.
Kaakoush, NO; Mitchell, HM and Man, SM (2015). Campylobacter. Mol. Med. Microbiol., 1187-1236. https://doi.org/10.1016/B978-0-12-397169-2.00067-6.
Kashoma, IP; Kumar, A; Sanad, YM; Gebreyes, W; Kazwala, RR; Garabed, R and Rajashekara, G (2014). Phenotypic and genotypic diversity of thermophilic Campylobacter spp. in commercial turkey flocks: a longitudinal study. Foodborne Pathog. Dis., 11: 850-860. https://doi.org/10.1089/fpd.2014.1794.
Khan, JA; Rathore, RS; Abulreesh, HH; Qais, FA and Ahmad, I (2018). Prevalence and antibiotic resistance profiles of Campylobacter jejuni isolated from poultry meat and related samples at retail shops in Northern India. Foodborne Pathog. Dis., 15: 218-225. https://doi.org/10. 1089/fpd.2017.2344.
Konkel, ME; Gray, SA; Kim, BJ; Garvis, SG and Yoon, J (1999). Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J. Clin. Microbiol., 37: 510-517.
Koolman, L; Whyte, P; Burgess, C and Bolton, D (2015). Distribution of virulence-associated genes in a selection of Campylobacter isolates. Foodborne Pathog. Dis., 12: 424-432. http://dx.doi.org/10.1089/fpd.2014.1883.
Kuana, SL; Santos, LR; Rodrigues, LB; Borsoi, A; Moraes, HLS; Salle, CTP and Nascimento, VP (2008). Occurrence and characterization of Campylobacter in the Brazilian production and processing of broilers. Avian Dis., 52: 680-684. https://doi.org/10.1637/8296-032608-Reg.1.
Ladely, SR; Berrang, ME; Meinersmann, RJ and Cox, NA (2017). Campylobacter multi-locus sequence types and antimicrobial susceptibility of broiler cecal isolates: A two year study of 143 commercial flocks. J. Food Saf., 37: e12366. https://doi.org/10.1111/jfs.12366.
Livestock Census of India (2019). Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Government of India, Krishi Bhavan, New Delhi.
Mossong, J; Mughini-Gras, L; Penny, C; Devaux, A; Olinger, C; Losch, S; Cauchie, HM; van Pelt, W and Ragimbeau, C (2016). Human campylobacteriosis in Luxembourg, 2010-2013: a case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. Sci. Rep., 6: 1-12. https://doi.org/ 10.1038/srep20939.
Musgrove, MT; Berrang, ME; Byrd, JA; Stern, NJ and Cox, NA (2001). Detection of Campylobacter spp. in ceca and crops with and without enrichment. Poult. Sci., 80: 825-828. https://doi.org/10.1093/ps/80.6.825.
NARMS (National Antimicrobial Resistance Monitoring System) (2020). U.S. Department of Health and Human Services, Food and Drug Administration. Rockville, MD. https://www.fda.gov/animal-veterinary/antimicrobial-resistance/nationalantimicrobial-resistance-monitoring-system.
Romero, MR and Cook, N (2018). A rapid LAMP-based method for screening poultry samples for Campylobacter without enrichment. Front. Microbiol., 9: 2401. https://doi. org/10.3389/fmicb.2018.02401.
Rossler, E; Olivero, C; Soto, LP; Frizzo, LS; Zimmermann, J; Rosmini, MR; Sequeira, GJ; Signorini, ML and Zbrun, MV (2020). Prevalence, genotypic diversity and detection of virulence genes in thermotolerant Campylobacter at different stages of the poultry meat supply chain. Int. J. Food Microbiol., 326: 108641. https://doi.org/10.1016/j.ijfoodmicro.2020.108641.
Sahin, O; Zhang, Q; Meitzler, JC; Harr, BS; Morishita, TY and Mohan, R (2001). Prevalence, antigenic specificity, and bactericidal activity of poultry anti-Campylobacter maternal antibodies. Appl. Environ. Microbiol., 67: 3951-3957. http://dx.doi.org/10.1128/aem.67.9.3951-3957.2001.
Sallam, KI (2007). Prevalence of Campylobacter in chicken and chicken by-products retailed in Sapporo area, Hokkaido, Japan. Food Control. 18: 1113-1120. http://dx.doi.org/10.1016/j.foodcont.2006.07.005.
Skarp, CPA; Hänninen, ML and Rautelin, HIK (2016). Campylobacteriosis: the role of poultry meat. Clin. Microbiol. Infect., 22: 103-109. http://dx.doi.org/10.1016/j. cmi.2015.11.019.
Tang, Y; Jiang, Q; Tang, H; Wang, Z; Yin, Y; Ren, F; Kong, L; Xinan Jiao, X and Huang, J (2020). Characterization and prevalence of Campylobacter spp. from broiler chicken rearing period to the slaughtering process in eastern China. Front. Vet. Sci., 7: 227. http://dx.doi.org/10.3389/fvets.2020.00227.
Toplak, N; Kovač, M; Piskernik, S; Možina, SS and Jeršek, B (2012). Detection and quantification of Campylobacter jejuni and Campylobacter coli using real-time multiplex PCR. J. Appl. Microbiol., 112: 752-764. http://dx.doi.org/ 10.1111/j.1365-2672.2012.05235.x.
Torralbo, A; Borge, C; García-Bocanegra, I; Méric, G; Perea, A and Carbonero, A (2015). Higher resistance of Campylobacter coli compared to Campylobacter jejuni at chicken slaughterhouse. Comp. Immunol. Microbiol. Infect. Dis., 39: 47-52. http://dx.doi.org/10.1016/j.cimid. 2015.02.003.
Ugarte-Ruiz, M; Gomez-Barrero, S; Porrero, MC; lvarez, JA; Garci, M; Comeron, MC; Wassenaar, TM and Dominguez, L (2012). Evaluation of four protocols for the detection and isolation of thermophilic Campylobacter from different matrices. J. Appl. Microbiol., 113: 200-208. doi: 10.1111/j.1365-2672.2012.05323.x.
Vasiliki, I; Ioannidis, A; Magiorkinis, E; Bagos, P; Nicolaou, C; Legakis, N and Chatzipanagiotou, S (2013). Multilocus sequence typing (and phylogenetic analysis) of Campylobacter jejuni and Campylobacter coli strains isolated from clinical cases in Greece. BMC Res. Notes. 6: 359. http://dx.doi.org/10.1186/1756-0500-6-359.
Vinueza-Burgos, C; Wautier, M; Martiny, D; Cisneros, M; Van Damme, I and De Zutter, L (2017). Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age. Poult. Sci., 96: 2366-2374. http://dx.doi.org/10.3382/ps/pew487.
Walker, LJ; Wallace, RL; Smith, JJ; Graham, T; Saputra, T; Symes, S; Stylianopoulos, A; Polkinghorne, BG; Kirk, MD and Glass, K (2019). Prevalence of Campylobacter coli and Campylobacter jejuni in retail chicken, beef, lamb, and pork products in three Australian states. J. Food Prot., 82: 2126-2134. doi: 10.4315/0362-028X.JFP-19-146.
Wang, G; Clifford, GC; Tracy, M; Pucknell, C; Barton, C; Price, L; Woodward, DL and Rodgers, FG (2002). Colony Multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J. Clin. Microbiol., 40: 4744-4747. http://dx.doi.org/10.1128/jcm.40.12.4744-4747.2002.
Wangroongsarb, P; Cheunban, N; Jittaprasatsin, C; Kamthalang, T; Saipradit, N; Chaichana, P; Chaiwat, P; Sittiporn, P and Orapan, S (2021). Prevalence and antimicrobial susceptibility of Campylobacter isolated from retail chickens in Thailand. Int. J. Food Microbiol., 339: 109017. http://dx.doi.org/10.1016/j.ijfoodmicro.2020. 109017. Wieczorek, K; Bocian, Ł and Osek, J (2020). Prevalence and antimicrobial resistance of Campylobacter isolated from carcasses of chickens slaughtered in Poland–a retrospective study. Food Control. 11: 107159. http://dx.doi.org/10.1016/ j.foodcont.2020.107159.
Wysok, B; Wojtacka, J and Kivistö, R (2020). Pathogenicity of Campylobacter strains of poultry and human origin from Poland. Int. J. Food Microbiol., 334: 108830. https://doi. org/10.1016/j.ijfoodmicro.2020.108830.
Zheng, J; Meng, J; Zhao, S; Singh, R and Song, W (2006). Adherence to and invasion of human intestinal epithelial cells by Campylobacter jejuni and Campylobacter coli isolates from retail meat products. J. Food Prot., 69: 768-774. | ||
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