| تعداد نشریات | 24 |
| تعداد شمارهها | 852 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,420,820 |
| تعداد دریافت فایل اصل مقاله | 11,583,444 |
First substantiation of clinico-molecular investigation of pathogenic Listeria monocytogenes in Nili-Ravi buffaloes | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 2 - شماره پیاپی 79، 2022، صفحه 120-127 اصل مقاله (491.32 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.41671.6062 | ||
| نویسندگان | ||
| M. Z. Munir1؛ J. A. Khan* 2؛ M. Ijaz2؛ F. Akhtar3 | ||
| 1Ph.D. Student in Veterinary Medicine, Department of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary, and Animal Sciences, Lahore, 54000, Pakistan | ||
| 2Department of Veterinary Medicine, Faculty of Veterinary Sciences, University of Veterinary, and Animal Sciences, Lahore, 54000, Pakistan | ||
| 3Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary, and Animal Sciences, Lahore, 54000, Pakistan | ||
| چکیده | ||
| Background: Listeriosis is a zoonotic disease of humans, animals, birds, fish, and crustaceans worldwide. Domestic animals, especially ruminants, are more susceptible to listeriosis. This infectious disease is caused by Listeria monocytogenes, an intracellular bacterium that can cross blood-brain, placental and intestinal barriers. In Pakistan, the incidence and reliable diagnostic tools for the L. monocytogenes are unidentified in Nili-Ravi buffaloes. Aims: This study was designed to inspect listeriosis in buffaloes through molecular techniques and haemato-biochemical analyses. Methods: A total of 230 samples (115 milk and 115 faecal samples) were collected from symptomatic listeriosis cases in Nili-Ravi buffaloes of 3 geographical districts (Rawalpindi, Faisalabad, and Muzaffargarh) Punjab, Pakistan. These samples were processed for DNA extraction using commercialized kits, and L. monocytogenes was confirmed by conventional PCR. Results: The results revealed that 6.08% and 4.34% of the isolates from milk and faecal samples were found positive for L. monocytogenes, respectively. The phylogenetic analysis of these isolates showed 97-100% similarity to isolates from the USA, Switzerland, Japan, and India. The accession numbers on NCBI GenBank appeared as HF558398 (Switzerland), KP965732 (India), EU372032 (USA), and LC259850 (Japan). Haemato-biochemical examinations showed that the values of WBCs, plasma fibrinogen, ALT, and AST significantly increased (P<0.05) in diseased buffaloes compared to healthy ones. Conclusion: The occurrence of listeriosis in buffaloes urges continuous monitoring and surveillance to prevent this emerging disease in Pakistan. | ||
| کلیدواژهها | ||
| Buffaloes؛ Listeria monocytogenes؛ Listeriosis؛ Phylogeny؛ Polymerase chain reaction | ||
| مراجع | ||
|
Amagliani, G; Omiccioli, E; Del Campo, A; Bruce, IJ; Brandi, G and Magnani, M (2006). Development of a magnetic capture hybridization PCR assay for Listeria monocytogenes direct detection in milk samples. J. Appl. Microbiol., 100: 375-383.
Aygun, O and Pehlivanlar, SJ (2006). Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control. 17: 676-679.
Barbuddhe, S; Chaudhari, S and Malik, S (2002). The occurrence of pathogenic Listeria monocytogenes and antibodies against listeriolysin O in buffaloes. J. Vet. Med. B., 49: 181-184.
Braun, U; Stehle, C and Ehrensperger, E (2002). Clinical findings and treatment of listeriosis in 67 sheep and goats. Vet. Rec., 150: 38-42.
Chandio, TH; Soomro, AH; Bhutto, MB; Dewani, P and Shah, GJ (2007). Occurrence of Listeria monocytogenes in bovine milk in Hyderabad, Pakistan. Ann. Microbiol., 57: 341-344.
Dehkordi, FS; Barati, S; Momtaz, H; Ahari, SNH and Dehkordi, SN (2013). Comparison of shedding, and antibiotic resistance properties of Listeria monocytogenes isolated from milk, feces, urine, and vaginal secretion of bovine, ovine, caprine, buffalo, and camel species in Iran. Jundishapur J. Microbiol., 6: 284-294.
El Hag, MM; El Zubeir, I and Mustafa, NE (2021). Prevalence of Listeria species in dairy farms in Khartoum State (Sudan). Food Control. 123: 107699.
El-Liethy, MA; Hemdan, BA and El-Taweel, GE (2020). Prevalence of E. coli, Salmonella, and Listeria spp. as potential pathogens: A comparative study for biofilm of sink drain environment. J. Food Saf., 40: e12816.
Esteban, JI; Oporto, B; Aduriz, G; Juste, RA and Hurtado, A (2009). Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain. BMC Vet. Res., 5: 1-10.
Fox, E; O’MAHONY, T; Clancy, M; Dempsey, R; O’BRIEN, M and Jordan, K (2009). Listeria monocytogenes in the Irish dairy farm environment. J. Food Prot., 72: 1450-1456.
Haj Hosseini, A; Sharifan, A and Tabatabaee, A (2014). Isolation of Listeria monocytogenes from meat and dairy products. JoMMID., 2: 159-162.
Headley, SA; Fritzen, JT; Queiroz, GR; Oliveira, RA; Alfieri, AF; Di Santis, GW; Lisbôa, JA and Alfieri, AA (2014). Molecular characterization of encephalitic bovine listeriosis from southern Brazil. Trop. Anim. Health Prod., 46: 19-25.
Hunt, K; Drummond, N; Murphy, M; Butler, F; Buckley, J and Jordan, K (2012). A case of bovine raw milk contamination with Listeria monocytogenes. Ir. Vet. J., 65: 1-5.
Kalender, H (2003). Detection of Listeria monocytogenes in faeces from chickens, sheep and cattle in Elazığ province. Turkish J. Vet. Anim. Sci., 27: 449-451.
Kalorey, DR; Warke, SR; Kurkure, NV; Rawool, DB and Barbuddhe, SB (2008). Listeria species in bovine raw milk: A large survey of Central India. Food Control. 19: 109-112.
Kennerman, E (2014). Determination of seroprevalence of Listeria monocytogenes antibodies in cattle in Bursa province of Turkey. Vet. Fak. Derg., 24: 95-98.
Konosonoka, I; Jemeljanovs, A; Osmane, B; Ikauniece, D and Gulbe, GJ (2012). Incidence of Listeria spp. in dairy cows feed and raw milk in Latvia. Int. Sch. Res. Notices. 2012.
Laximan, S; Kaur, S; Aulakh, RS and Gill, JPS (2016). Molecular characterization of Listeria monocytogenes in bovine milk and evaluating the sensitivity of PCR for direct detection in milk. Indian J. Anim. Sci., 86: 512-517.
Lecuit, M (2005). Understanding how Listeria monocytogenes targets and crosses host barriers. Clin. Microbiol. Infect., 11: 430-436.
Liu, D; Lawrence, ML; Austin, FW and Ainsworth, AJ (2007). A multiplex PCR for species-and virulence-specific determination of Listeria monocytogenes. J. Microbiol. Methods. 71: 133-140.
Mansouri-Najand, L; Kianpour, M; Sami, M and Jajarmi, M (2015). Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran. Vet. Res. Forum., 6: 223.
Meyer-Broseta, S; Diot, A; Bastian, S; Rivière, J and Cerf, O (2003). Estimation of low bacterial concentration: Listeria monocytogenes in raw milk. Int. J. Food Microbiol., 80: 1-15.
Nayak, DN; Savalia, CV; Kshirsagar, DP and Kumar, R (2015). Isolation, identification and molecular characterization of Listeria species from milk and milk products in Navsari city of South Gujarat. J. Vet. Pub. Health. 13: 19-23.
Nightingale, KK; Fortes, ED; Ho, AJ; Schukken, YH; Grohn, YT and Wiedmann, M (2005). Evaluation of farm management practices as risk factors for clinical listeriosis and fecal shedding of Listeria monocytogenes in ruminants. J. Am. Vet. Med. Assoc., 227: 1808-1814.
Orsi, RH and Wiedmann, M (2016). Characteristics and distribution of Listeria spp., including Listeria species newly described since 2009. Appl. Microbiol. Biotechnol., 100: 5273-5287.
Osman, KM; Samir, A; Abo-Shama, UH; Mohamed, EH; Orabi, A and Zolnikov, T (2016). Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk. BMC Microbiol., 16: 1-13.
Paziak-Domańska, B; Bogusławska, E; Więckowska-Szakiel, M; Kotłowski, R; Różalska, B; Chmiela, M; Kur, J; Dąbrowski, W and Rudnicka, W (1999). Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiol. Lett., 171: 209-214.
Prado, RGS; Domiciano, TAO; Paredes, LJA; Bezerra, PS; Pereira, WLA; Cerqueira, VD; Driemeier, D and Riet-Correa, G (2019). Nervous form of listeriosis in buffaloes. Pesqui. Vet. Bras., 39: 299-303.
Quereda, JJ; Leclercq, A; Moura, A; Vales, G; Gómez-Martín, Á; García-Muñoz, Á; Thouvenot, P; Tessaud-Rita, N; Bracq-Dieye, H and Lecuit, M (2020). Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep. Int. J. Syst. Evol. Microbiol., 70: 5868-5879.
Rahimi, E; Momtaz, H; Behzadnia, A and Baghbadorani, Z (2014). Incidence of Listeria species in bovine, ovine, caprine, camel and water buffalo milk using cultural method and the PCR assay. Asian Pac. J. Trop. Dis., 4: 50-53.
Samad, A; Abbas, F; Ahmad, Z; Pokryrshko, O and Asmat, TMJ (2018). Prevalence of foodborne pathogens in food items in Quetta, Pakistan. Pak. J. Zool., 50: 1-4.
Schweizer, G; Ehrensperger, F; Torgerson, PR and Braun, U (2006). Clinical findings and treatment of 94 cattle presumptively diagnosed with listeriosis. Vet. Rec., 158: 588-592.
Shakuntala, I; Das, S; Ghatak, S; Milton, AAP; Sanjukta, R; Puro, KU; Pegu, RK; Duarah, A; Barbuddhe, SB and Sen, A (2019). Prevalence, characterization, and genetic diversity of Listeria monocytogenes isolated from foods of animal origin in North East India. Food Biotechnol., 33: 237-250.
Shakuntala, I; Malik, S; Barbuddhe, S and Rawool, DJ (2006). Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Vet. Microbiol., 117: 229-234.
Shamloo, E; Hosseini, H; Moghadam, ZA; Larsen, MH; Haslberger, A and Alebouyeh, M (2019). Importance of Listeria monocytogenes in food safety: a review of its prevalence, detection, and antibiotic resistance. Iran. J. Vet. Res., 20: 241.
Shantha, S and Gopal, S (2014). Prevalence of Listeria species in environment and milk samples. Adv. Anim. Vet. Sci., 2: 1-4.
Silk, BJ; Mahon, BE; Griffin, PM; Gould, LH; Tauxe, RV; Crim, SM; Jackson, KA; Gerner-Smidt, P; Herman, KM and Henao, OL (2013). Vital signs: Listeria illnesses, deaths, and outbreaks—United States, 2009-2011. MMWR Morb. Mortal Wkly Rep., 62: 448.
Skowron, K; Wałecka-Zacharksa, E; Grudlewska, K; Wiktorczyk, N; Kaczmarek, A; Gryń, G; Kwiecińska-Piróg, J; Juszczuk, K; Paluszak, Z and Kosek-Paszkowska, K (2019). Characteristics of Listeria monocytogenes strains isolated from milk and humans and the possibility of milk-borne strains transmission. Pol. J. Microbiol., 68: 353-369.
Sleator, RD; Gahan, CG and Hill, C (2003). A postgenomic appraisal of osmotolerance in Listeria monocytogenes. Appl. Environ. Microbiol., 69: 1-9.
Soni, DK and Dubey, SKJ (2014). Phylogenetic analysis of the Listeria monocytogenes based on sequencing of 16S rRNA and hlyA genes. Mol. Biol. Rep., 41: 8219-8229.
Soni, DK; Singh, RK; Singh, DV and Dubey, SK (2013). Characterization of Listeria monocytogenes isolated from Ganges water, human clinical and milk samples at Varanasi, India. Infect. Genet. Evol., 14: 83-91.
Terzi Gulel, G; Gucukoglu, A; Cadirci, O; Saka, E and Alisarli, MJ (2020). Serotyping and antibiotic resistance of Listeria monocytogenes isolated from raw water buffalo milk and milk products. J. Food Sci., 85: 2889-2895.
Usman, UB; Kwaga, JKP; Kabir, J; Olonitola, OS; Radu, S and Bande, F (2016). Molecular characterization and phylogenetic analysis of Listeria monocytogenes isolated from milk and milk products in Kaduna, Nigeria. Can. J. Infect. Dis. Med. Microbiol., 2016.
Usman, M and Mukhtar, N (2014). Prevalence of Listeria monocytogenese in raw milk in Faisalabad, Pakistan. IOSR J. Agric. Vet. Sci., 7: 08-11.
Vázquez-Boland, JA; Kuhn, M; Berche, P; Chakraborty, T; Domı́nguez-Bernal, G; Goebel, W; González-Zorn, B; Wehland, Jr and Kreft, Jr (2001). Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev., 14: 584-640.
Vilar, MJ; Yus, E; Sanjuan, ML; Diéguez, F and Rodríguez-Otero, J (2007). Prevalence and risk factors for Listeria species on dairy farms. J. Dairy Sci., 90: 5083-5088.
Walland, J; Lauper, J; Frey, J; Imhof, R; Stephan, R; Seuberlich, T and Oevermann, A (2015). Listeria monocytogenes infection in ruminants: is there a link to the environment, food and human health? A review. Schweiz. Arch. Tierheilkd., 157: 319-328.
Zafar, N (2020). Prevalence, molecular characterization and antibiogram study of Listeria monocytogenes isolated from raw milk and milk products. Pure Appl. Biol., 9: 1982-1987. | ||
|
آمار تعداد مشاهده مقاله: 594 تعداد دریافت فایل اصل مقاله: 647 |
||