| تعداد نشریات | 24 |
| تعداد شمارهها | 917 |
| تعداد مقالات | 8,230 |
| تعداد مشاهده مقاله | 16,274,422 |
| تعداد دریافت فایل اصل مقاله | 13,750,851 |
Intrauterine proteolytic enzymes therapy hastens expulsion of fetal membranes in dystocia affected buffaloes | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 2 - شماره پیاپی 79، 2022، صفحه 163-168 اصل مقاله (402.48 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.41684.6066 | ||
| نویسندگان | ||
| N. Gulia1؛ M. Honparkhe* 2؛ A. Bisla2؛ A. K. Singh2؛ P. Singh3 | ||
| 1Ph.D. Student in Veterinary Gynaecology and Obstetrics, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| 2Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| 3Department of Teaching Veterinary Clinical Complex, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| چکیده | ||
| Background: Difficult calving (dystocia) in buffalo cows is a major obstetrical problem which further leads to metritis complex, encompassing the retention of fetal membranes (RFM), puerperal metritis, endometritis and pyometra with impaired future fertility. Aims: The current study aimed to evaluate the effect of the administration of intrauterine proteolytic enzymes on the expulsion of fetal membranes and postpartum fertility in dystociac buffaloes. Methods: Proteolytic enzymes consisting of Trypsin (16 mg), Chymotrypsin (16 mg), and Papain (8 mg) were dissolved in 500 ml normal saline were administered after 1 h of assisted delivery in dystociac buffaloes along with the conventional therapy. Results: The treated animals (n=15) expelled fetal membranes within a shorter period of time (P=0.043) compared to the control group (n=15) with none in the treatment group retaining it for more than 24 hours. Fewer (26.67 vs 73.33%; P=0.027) postpartum uterine infections developed in the treated animals compared to the control group. The interval between first postpartum estrus (P=0.067), service period (P=0.554), and open days (P=0.557) was shorter in the treatment group compared to the control group where postpartum anestrus developed less frequently (26.67 vs 66.67%; P=0.066) in the animals treated with enzymatic therapy. Systemic illness (neutrophillia) was reduced in the treatment group compared to the control on day 20 (64.55 ± 1.14% vs 70.23 ± 0.99%; P=0.001) and 45 (55.05 ± 1.63% vs 64.92 ± 1.45%; P<0.001) postpartum. Conclusion: It is concluded that proteolytic enzymes therapy after assisted delivery in dystociac buffalo cows could help in the early expulsion of fetal membranes and reduce uterine infections with decreased neutrophils count. | ||
| کلیدواژهها | ||
| Buffalo؛ Dystocia؛ Placenta؛ Proteolytic enzymes | ||
| مراجع | ||
|
20th Livestock Census (2019). All India Report. Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture and Farmers Welfare, Government of India, New Delhi. https://dahd.nic.in/documents/statistics/ livestock-census.
Au, YP; Montgomery, KF and Clowes, AW (1992). Heparin inhibits collagenase gene expression mediated by phorbol ester-responsive element in primate arterial smooth muscle cells. Circ. Res., 70: 1062-1069.
Azawi, O; Rahawy, M and Hadad, J (2008). Bacterial isolates associated with dystocia and retained placenta in Iraqi buffaloes. Reprod. Domest. Anim., 43: 286-292.
Beagley, JC; Whitman, KJ; Baptiste, KE and Scherzer, J (2010). Physiology and treatment of retained fetal membranes in cattle. J. Vet. Int. Med., 24: 261-268.
Bhavna; Nishi, P; Rajesh, A and Utsav, S (2019). Evaluation and modulation of postpartum uterine health using proteolytic enzymes in crossbred dairy cows. Har. Vet., 58: 124-126.
DAHD Annual Report (2020-21). Retrieved from https:// dahd.nic.in/annual_report.
Drillich, M; Raab, D; Miriam, W and Heuwieser, W (2005). Treatment of chronic endometritis in dairy cattle with an intrauterine application of enzymes: A field trial. Theriogenology. 63: 1811-1823.
Dubuc, J; Duffield, TF; Leslie, KE; Walton, JS and LeBlanc, SJ (2010). Risk factors for postpartum uterine diseases in dairy cows. J. Dairy Sci., 93: 5764-5771.
Eiler, H and Fecteau, KA (2007). Retained placenta. Current therapy in large animal theriogenology. 2nd Edn., St. Louis, Missouri, USA, Elservier. PP: 345-354.
Eiler, H and Hopkins, FM (1992). Bovine retained placenta: effects of collagenase and hyaluronidase on detachment of placenta. Biol. Reprod., 46: 580-585.
El-Wishy, AB (2007a). The postpartum buffalo: I. Endocrinological changes and uterine involution. Anim. Reprod. Sci., 97: 201-215.
El-Wishy, AB (2007b). The postpartum buffalo II. Acyclicity and anestrus. Anim. Reprod. Sci., 97: 216-236.
Fecteau, KA and Eiler, H (2001). Placenta detachment: Unexpected high concentrations of 5-hydroxytryptamine (serotonin) in fetal blood and its mitogenic effect on placental cells in bovine. Placenta. 22: 103-110.
Gautam, RP (2000). Studies on retention on fetal membranes in buffaloes, effect of some drugs on uterine contraction in vitro and expulsion of fetal membrane. M.V.Sc. Thesis, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India. PP: 1-68.
Gohar, MA; Elmetwally, MA; Montaser, A and Zaabel, SM (2018). Effect of oxytetracycline treatment on postpartum reproductive performance in dairy buffalo-cows with retained placenta. Egyptian J. Vet. Health. 1: 45-53.
Gröhn, YT and Rajala-Schultz, PJ (2000). Epidemiology of reproductive performance in dairy cows. Anim. Reprod. Sci., 60: 605-614.
Gross, TS (1985). In vitro proteolytic activity of the late pregnant and peripartum bovine placenta. J. Anim. Sci., 61: 391-429.
Gunnick, JW (1984). Retained placenta and leukocytic activity. Vet. Quart., 6: 49-51.
Honparkhe, M; Ghuman, SPS; Singh, J; Dhindsa, SS; Kumar, A; Chandra, M and Brar, PS (2014). Diagnosing sub-clinical endometritis through uterine cytobrush cytology and its treatment with proteolytic enzymes in buffaloes. In: XXIX Annual Convention and National Symposium of Indian Society for Study of Animal Reproduction on “Frontier Reproductive Biotechnologies for Enhancing Animal Fertility and Fecundity: Global perspective”, Maharashtra Animal and Fishery Science University, Nagpur, India, January 8-10. P: 147.
Honparkhe, M; Singh, J; Dadarwal, D; Kang, RS and Dhaliwal, GS (2005). Comparative efficacy of various intrauterine therapies in repeat breeding cross bred cattle with uterine infections. In: XXI Annual Convention and National Symposium of Indian Society for Animal Reproduction on “Recent trends and innovation in Animal Reproduction”. Faculty of Veterinary Science and Animal Husbandry, at Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, India, November 23-25. PP: 103-104.
Indurkar, S; Tiwari, RP; Dubey, M; Khan, JR and Mishra, GK (2019). Biochemical and hormonal profiles in buffaloes with retained fetal membranes. Buff. Bull., 38: 35-39.
Jadon, RS; Dhaliwal, GS and Jand, SK (2005). Prevalence of aerobic and anaerobic uterine bacteria during peripartum period in normal and dystocia affected buffaloes. Anim. Reprod. Sci., 88: 215-224.
Kruger, M; Hien, TT; Zaremba, W and Penka, L (1999). Investigations on the influence of the proteolytic enzymes trypsin, chymotrypsin and papain on udder pathogenic microorganisms. Part 1: Influence of enzymes on growth behaviour, survival rate, chain formation (streptococci) and morphism (yeasts). Tierarztl. Prax. Ausg. G: Grosstiere - Nutztiere., 27: 207-215. Qureshi, ZI; Lodhi, LA and Sattar, A (1997). An apparent effect of immunopotentiation during late gestation on the postpartum reproductive performance of Nili-Ravi buffaloes (Bubalus bubalis). Vet. Res. Commun., 21: 375-380.
Rajala, PJ and Gröhn, YT (1998). Effects of dystocia, retained placenta, and metritis on milk yield in dairy cows. J. Dairy Sci., 81: 3172-3181.
Shalaby, AS; Sharawy, SM; Saleh, NH and Hassan, EA (1994). Effect of orostaglandin F2 a on the expulsion of the placenta, and the interval from birth to the first postpartum estrus in buffaloes. Assiut Vet. Med. J., 31: 320.
Sheldon, IM; Cronin, J; Goetze, L; Donofrio, G and Schuberth, HJ (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol. Reprod., 81: 1025-1032.
Sheldon, IM; Lewis, GS; LeBlanc, S and Gilbert, RO (2006). Defining postpartum uterine disease in cattle. Theriogenology. 65: 1516-1530.
Singh, AK; Bansal, AK; Prabhakar, S; Cheema, RS; Singh, P; Brar, PS and Gandotra, VK (2011). Level of cortisol in placental tissue vis-à-vis oxidative stress in dystocia affected buffaloes. Indian J. Anim. Sci., 81: 235-239.
Singh, H; Brar, PS; Singh, N; Jan, MH; Honparkhe, M and Dhindsa, SS (2020a). Effects of intra-uterine infusion of proteolytic enzymes on selected cytokine concentrations, uterine inflammation, and fertility in postpartum water buffalo cows with subclinical endometritis. Anim. Reprod. Sci., 215: 1-9.
Singh, A; Dhindsa, SS; Singh, P; Singh, G and Honparkhe, M (2020b). Effect of prostaglandin and gonadotropin releasing hormone on uterine involution in dystocia affected buffaloes. Int. J. Curr. Microbiol. Appl. Sci., 9: 318-323.
Singh, J; Honparkhe, M; Ghuman, SPS; Kumar, A; Dhindsa, S and Chandra, M (2017). Intrauterine proteolytic enzyme therapy for subclinical endometritis in dairy cattle. Indian J. Anim. Reprod., 38: 1-3.
Usmani, RH; Ahmad, N; Shafiq, P and Mirza, MA (2001). Effect of subclinical uterine infection on cervical and uterine involution, estrous activity and fertility in postpartum buffaloes. Theriogenology. 55: 563-571.
Vala, KB; Kavani, FS; Raval, RJ and Tank, PH (2018). Evaluation of uterine involution by rectal palpation and ultrasonography in peripartum nutritional supplemented Jaffarabadi buffaloes. Int. J. Curr. Microbiol. App. Sci., 7: 1955-1963.
Wani, AA; Bhat, MB; Mavi, PS and Brar, PS (2019). Effect of oxytocin, pgf2α and GnRH on uterine involution and postpartum fertility in murrah buffaloes subjected to fetotomy. Indian J. Anim. Reprod., 40: 35-37.
Zander, H (1997). Examinations about the effectiveness of enzymes and enzyme/antibiotic-combinations in the therapy of different kind of bovine mastitis. Doctoral Dissertation, Thesis Freie Universität Berlin. | ||
|
آمار تعداد مشاهده مقاله: 961 تعداد دریافت فایل اصل مقاله: 826 |
||