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Dolatshah, Leila, Tabatabaei, Mohammad. (1400). A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa. , 10(4), 157-163. doi: 10.22099/mbrc.2021.41708.1673
Leila Dolatshah; Mohammad Tabatabaei. "A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa". , 10, 4, 1400, 157-163. doi: 10.22099/mbrc.2021.41708.1673
Dolatshah, Leila, Tabatabaei, Mohammad. (1400). 'A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa', , 10(4), pp. 157-163. doi: 10.22099/mbrc.2021.41708.1673
Dolatshah, Leila, Tabatabaei, Mohammad. A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa. , 1400; 10(4): 157-163. doi: 10.22099/mbrc.2021.41708.1673

A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa

Molecular Biology Research Communications
دوره 10، شماره 4، 2021، صفحه 157-163    اصل مقاله (301.58 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2021.41708.1673
نویسندگان
Leila Dolatshah؛ Mohammad Tabatabaei*
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده
Pseudomonas aeruginosa is identified as a versatile opportunistic microorganism with metabolic diversity contributing to a wide range of health burdens, especially in immunocompromised patients. This bacterium is the cause of 10 to 20% of nosocomial infections. In this study, we evaluated the phenotypic characterizations of biofilm formation in P. aeruginosa clinical isolates using micro-titer plate assay. Indeed, we estimated the prevalence of QS (rhlI, rhlR, rhlAB, lasB, lasI, lasR, aprA) and virulence genes (pslA and cupA) by PCR. The results showed that among 69% of the isolates forming biofilm, 9% were strong biofilm producers, whereas 13% and 47% of isolates produced moderate and low amounts of biofilm, respectively. All isolates possessed cupA and seven QS genes (rhlI, rhlR, rhlAB, lasB,  lasI, lasR, aprA), while 92% of the isolates possessed the pslA gene. Identification of these genes and their association with biofilm formation can be advantageous in adopting therapeutic methods.
کلیدواژه‌ها
Pseudomonas aeruginosa؛ Biofilm؛ Quorum sensing؛ Fimbrial؛ cupA؛ pslA
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