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de Moraes, Rodolfo, Pimenta, Ruan, Mori, Fernando, dos Santos, Gabriel, Viana, Nayara, Guimarães, Vanessa, de Camargo, Juliana, Leite, Katia, Srougi, Miguel, Nahas, William, Reis, Sabrina. (1400). Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?. , 10(4), 149-156. doi: 10.22099/mbrc.2021.40912.1646
Rodolfo Pacheco de Moraes; Ruan Pimenta; Fernando Noburu Cabral Mori; Gabriel Arantes dos Santos; Nayara Izabel Viana; Vanessa Ribeiro Guimarães; Juliana Alves de Camargo; Katia Ramos Moreira- Leite; Miguel Srougi; William Carlos Nahas; Sabrina T. Reis. "Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?". , 10, 4, 1400, 149-156. doi: 10.22099/mbrc.2021.40912.1646
de Moraes, Rodolfo, Pimenta, Ruan, Mori, Fernando, dos Santos, Gabriel, Viana, Nayara, Guimarães, Vanessa, de Camargo, Juliana, Leite, Katia, Srougi, Miguel, Nahas, William, Reis, Sabrina. (1400). 'Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?', , 10(4), pp. 149-156. doi: 10.22099/mbrc.2021.40912.1646
de Moraes, Rodolfo, Pimenta, Ruan, Mori, Fernando, dos Santos, Gabriel, Viana, Nayara, Guimarães, Vanessa, de Camargo, Juliana, Leite, Katia, Srougi, Miguel, Nahas, William, Reis, Sabrina. Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?. , 1400; 10(4): 149-156. doi: 10.22099/mbrc.2021.40912.1646

Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?

Molecular Biology Research Communications
دوره 10، شماره 4، 2021، صفحه 149-156    اصل مقاله (257.03 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2021.40912.1646
نویسندگان
Rodolfo Pacheco de Moraes1؛ Ruan Pimenta1، 2؛ Fernando Noburu Cabral Mori1؛ Gabriel Arantes dos Santos* 1، 2؛ Nayara Izabel Viana1؛ Vanessa Ribeiro Guimarães1؛ Juliana Alves de Camargo1؛ Katia Ramos Moreira- Leite1؛ Miguel Srougi1، 2؛ William Carlos Nahas3؛ Sabrina T. Reis1
1Laboratory of Medical Investigation (LIM55), Urology Department, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil
2D'Or Institute for Research and Education (IDOR), São Paulo, Brazil
3Instituto Do Cancer Do Estado De São Paulo (ICESP), Universidade de São Paulo, São Paulo, Brazil
چکیده
Prostate cancer is the most frequent malignancy affecting men worldwide. Due to the low sensitivity and specificity of the prostate-specific antigen test and the digital rectal exam as screening modalities, several alternatives are being studied. This study aimed to evaluate the application of MMP-9 and its regulators (TIMP-1, RECK, and miR-338-3p) as diagnostic and prognostic indicators of prostate cancer. A total of 134 randomly selected patients under investigation for prostate cancer submitted to a transrectal ultrasound-guided prostate biopsy were enrolled in the study; of these, 61 were positive for the disease (cases), and 73 were negative (control group). The tissue samples were further analyzed by gene and miR-338-3p expression analysis using qRT-PCR (one randomly selected fragment of each patient).  Approximately 58% of the patients with prostate cancer presented MMP9 upregulation, while 73%, 65%, and 69% downregulated IMP-1, RECK, and miR-338-3p, respectively. MiR-338-3p was expressed at lower levels in patients with PSA concentrations exceeding 20 ng/mL (p=0.045) and abnormal DRE (p=0.006), while the RECK was more expressed in patients with abnormal DRE (p=0.01). We found that most patients with prostate cancer overexpressed MMP-9; on the other hand, most of them underexpressed TIMP-1, RECK, and miR-338-3p. MiR-338-3p presented as a possible predictor of poor prognosis. Further studies are warranted to evaluate these biomarkers as prognosis factors better.
کلیدواژه‌ها
Image-guided biopsy؛ Biomarkers؛ Matrix metalloproteinases؛ TIMP proteins؛ MicroRNA
مراجع
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