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Immunization with recombinant PlpE of ovine Mannheimia haemolytica isolate provides protection against lethal challenge in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 4 - شماره پیاپی 77، 2021، صفحه 272-276 اصل مقاله (309.01 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.37923.5522 | ||
| نویسندگان | ||
| A. Yektaseresht* 1؛ Z. Hemati2؛ F. Sabet Sarvestani1؛ A. Hosseini1؛ S. Vir Singh3 | ||
| 1Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran | ||
| 3Department of Biotechnology, Institute of Applied Sciences and Humanities, GLA University, Ajhai, Mathura, Uttar Pradesh, India | ||
| چکیده | ||
| Background: Mannheimia haemolytica primarily causes pneumonia leading to heavy morbidity and mortality in the domestic livestock world-wide. Recently, lipoproteins have emerged as targets for inducing protective immunity against the Pasteurella infection. Aims: This study was aimed toevaluate recombinant outer membrane lipoprotein E (PlpE) from the isolate of ovine M. haemolytica, as potential vaccine candidate’. Methods: Recombinant PlpE was constructed using pET26 (b) expression vector in Escherichia coli. Expressed recombinant PlpE was purified and injected subcutaneously in the mice. Protection index of the vaccine was evaluated by challenge of mice intraperitoneally. Results: Anti-PlpE antibody responses in the immunized mice was significantly increased which provided effective protection, when challenged with strain of virulent M. haemolytica. Conclusion: Recombinant PlpE from ovine M. haemolytica isolate had potential to be used as ‘vaccine candidate against M. haemolytica infection in sheep flocks. | ||
| کلیدواژهها | ||
| Mannheimia haemolytica؛ PlpE؛ Recombinant vaccine؛ Sheep | ||
| مراجع | ||
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Aubry, P; Warnick, LD; Guard, CL; Hill, BW and Witt, MF (2001). Health and performance of young dairy calves vaccinated with a modified live Mannheimia haemolytica and Pasteurella multocida vaccine. J. Am. Vet. Med. Assoc., 219: 1739-1742.
Ayalew, S; Confer, AW; Payton, ME; Garrels, KD; Shrestha, B; Ingram, KR; Montelongo, MA and Taylor, JD (2008). Mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein PlpE and the neutralizing epitope of leukotoxin. Vaccine. 26: 4955-4961.
Batra, SA; Shanthalingam, S; Donofrio, G and Srikumaran, S (2016). A chimeric protein comprising the immunogenic domains of Mannheimia haemolytica leukotoxin and outer membrane protein PlpE induces antibodies against leukotoxin and PlpE. Vet. Immunol. Immunopathol., 175: 36-41.
Carpenter, T; Khalid, S and Sansom, MS (2007). A multi domain outer membrane protein from Pasteurella multocida: modelling and simulation studies of PmOmpA. Biochim. Biophys. Acta. 1768: 2831-2840.
Clinkenbeard, KD; Mosier, DA and Confer, AW (1989). Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin. Infect. Immun., 57: 420-425.
Confer, AW and Ayalew, S (2018). Mannheimia haemolytica in bovine respiratory disease: immunogens, potential immunogens, and vaccines. Anim. Health Res. Rev., 19: 79-99.
Confer, AW; Ayalew, S; Montelongo, M; Step, DL; Wray, JH; Hansen, RD and Panciera, RJ (2009). Immunity of cattle following vaccination with a Mannheimia haemolytica chimeric PlpE-LKT (SAC89) protein. Vaccine. 27: 1771-1776.
Confer, AW; Ayalew, S; Panciera, RJ; Montelongo, M; Whitworth, LC and Hammer, JD (2003). Immuno-genicity of recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE and augmentation of a commercial vaccine. Vaccine. 21: 2821-2829.
Confer, AW; Ayalew, S; Panciera, RJ; Montelongo, M and Wray, JH (2006). Recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE enhances commercial M. haemolytica vaccine-induced resistance against serotype 6 challenge. Vaccine. 24: 2248-2255.
Confer, AW; McCraw, RD; Durham, JA; Morton, RJ and Panciera, RJ (1995). Serum antibody responses of cattle to iron-regulated outer membrane proteins of Pasteurella haemolytica A1. Vet. Immunol. Immunopathol., 47: 101-110.
Conlon, JA; Shewen, PE and Lo, RY (1991). Efficacy of recombinant leukotoxin in protection against pneumonic challenge with live Pasteurella haemolytica A1. Infect. Immun., 59: 587-591.
Gatto, NT; Dabo, SM; Hancock, RE and Confer, AW (2002). Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multocida serotype A:3 (Omp28). Vet. Microbiol., 87: 221-235.
Guzman-Brambila, C; Quintero-Fabian, S; Gonzalez-Castillo, C; de Obeso-Fernandez del Valle, A; Flores-Samaniego, B; de la Mora, G; Rojas-Mayorquin, AE and Ortuno-Sahagun, D (2012). LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge. Res. Vet. Sci., 93: 1293-1300.
Hsuan, SL; Kannan, MS; Jeyaseelan, S; Prakash, YS; Malazdrewich, C; Abrahamsen, MS; Sieck, GC and Maheswaran, SK (1999). Pasteurella haemolytica leukotoxin and endotoxin induced cytokine gene expression in bovine alveolar macrophages requires NF-kappaB activation and calcium elevation. Microb. Pathogenesis. 26: 263-273.
Lee, HY; Kehrli Jr, ME; Brogden, KA; Gallup, JM and Ackermann, MR (2000). Influence of beta(2)-integrin adhesion molecule expression and pulmonary infection with Pasteurella haemolytica on cytokine gene expression in cattle. Infect. Immun., 68: 4274-4281.
Maheswaran, SK; Weiss, DJ; Kannan, MS; Townsend, EL; Reddy, K; Rwhiteley, LO and Srikumaran, S (1992). Effects of Pasteurella haemolytica A1 leukotoxin on bovine neutrophils: degranulation and generation of oxygen-derived free radicals. Vet. Immunol. Immunop., 33: 51-68.
Morton, RJ; Panciera, RJ; Fulton, RW; Frank, GH; Ewing, SA; Homer, JT and Confer, AW (1995). Vaccination of cattle with outer membrane protein-enriched fractions of Pasteurella haemolytica and resistance against experimental challenge exposure. Am. J. Vet. Res., 56: 875-879.
Ozbey, G and Muz, A (2006). Isolation of aerobic bacteria from the lungs of chickens showing respiratory disorders and confirmation of Pasteurella multocida by polymerase chain reaction (PCR). Vet. Arh., 76: 217-225.
Pandher, K; Confer, AW and Murphy, GL (1998). Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1. Infect. Immun., 66: 5613-5619.
Pandher, K; Murphy, GL and Confer, AW (1999). Identification of immunogenic, surface-exposed outer membrane proteins of Pasteurella haemolytica serotype 1. Vet. Microbiol., 65: 215-226.
Purdy, CW; Straus, DC; Sutherland, RJ and Ayres, JR (1996). Efficacy of a subcutaneously administered, ultraviolet light-killed Pasteurella haemolytica A1-containing vaccine against transthoracic challenge exposure in goats. Am. J. Vet. Res., 57: 1168-1174.
Quinn, PJ; Markey, BK; Leonard, FC; Hartigan, P; Fanning, S and Patrick, ESF (2011). Veterinary microbiology and microbial disease. UK, John Willy & Son Ltd.
Rajeev, S; Kania, SA; Nair, RV; McPherson, JT; Moore, RN and Bemis, DA (2001). Bordetella bronchiseptica fimbrial protein-enhanced immunogenicity of a Mannheimia haemolytica leukotoxin fragment. Vaccine. 19: 4842-4850.
Shewen, PE; Lee, CW; Perets, A; Hodgins, DC; Baldwin, K and Lo, RY (2003). Efficacy of recombinant sialoglycoprotease in protection of cattle against pneumonic challenge with Mannheimia (Pasteurella) haemolytica A1. Vaccine. 21: 1901-1906.
Shewen, PE and Wilkie, BN (1985). Evidence for the Pasteurella haemolytica cytotoxin as a product of actively growing bacteria. Am. J. Vet. Res., 46: 1212-1214.
Yektaseresht, A; Sabet Sarvestani, F; Dordani, M and Hosseini, A (2019). Cloning and expression of Mannheimia haemolytica PlpE gene in Escherichia coli and its immunogenicity assessment. Arch. Razi Inst., 74: 111-118. | ||
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