اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها903
تعداد مقالات8,081
تعداد مشاهده مقاله15,203,325
تعداد دریافت فایل اصل مقاله12,847,690
Pimenta, Ruan, Viana, Nayara, dos Santos, Gabriel, Candido, Patricia, Guimaraes, Vanessa, Romao, Poliana, Silva, Iran, de Camargo, Juliana, Hatanaka, Dina, Queiroz, Paula, Teruya, Alexandre, Echenique, Leandro, Besen, Bruno, Leite, Katia, Srougi, Victor, Srougi, Miguel, Reis, Sabrina. (1400). MiR-200c-3p expression may be associated with worsening of the clinical course of patients with COVID-19. , 10(3), 141-147. doi: 10.22099/mbrc.2021.40555.1631
Ruan Pimenta; Nayara I Viana; Gabriel A dos Santos; Patricia Candido; Vanessa R Guimaraes; Poliana Romao; Iran A Silva; Juliana A de Camargo; Dina M Hatanaka; Paula G S Queiroz; Alexandre Teruya; Leandro Echenique; Bruno A M P Besen; Katia R M Leite; Victor Srougi; Miguel Srougi; Sabrina T Reis. "MiR-200c-3p expression may be associated with worsening of the clinical course of patients with COVID-19". , 10, 3, 1400, 141-147. doi: 10.22099/mbrc.2021.40555.1631
Pimenta, Ruan, Viana, Nayara, dos Santos, Gabriel, Candido, Patricia, Guimaraes, Vanessa, Romao, Poliana, Silva, Iran, de Camargo, Juliana, Hatanaka, Dina, Queiroz, Paula, Teruya, Alexandre, Echenique, Leandro, Besen, Bruno, Leite, Katia, Srougi, Victor, Srougi, Miguel, Reis, Sabrina. (1400). 'MiR-200c-3p expression may be associated with worsening of the clinical course of patients with COVID-19', , 10(3), pp. 141-147. doi: 10.22099/mbrc.2021.40555.1631
Pimenta, Ruan, Viana, Nayara, dos Santos, Gabriel, Candido, Patricia, Guimaraes, Vanessa, Romao, Poliana, Silva, Iran, de Camargo, Juliana, Hatanaka, Dina, Queiroz, Paula, Teruya, Alexandre, Echenique, Leandro, Besen, Bruno, Leite, Katia, Srougi, Victor, Srougi, Miguel, Reis, Sabrina. MiR-200c-3p expression may be associated with worsening of the clinical course of patients with COVID-19. , 1400; 10(3): 141-147. doi: 10.22099/mbrc.2021.40555.1631

MiR-200c-3p expression may be associated with worsening of the clinical course of patients with COVID-19

Molecular Biology Research Communications
دوره 10، شماره 3، 2021، صفحه 141-147    اصل مقاله (359.81 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2021.40555.1631
نویسندگان
Ruan Pimenta* 1؛ Nayara I Viana1؛ Gabriel A dos Santos1؛ Patricia Candido1؛ Vanessa R Guimaraes1؛ Poliana Romao1؛ Iran A Silva1؛ Juliana A de Camargo1؛ Dina M Hatanaka2؛ Paula G S Queiroz2؛ Alexandre Teruya2؛ Leandro Echenique2؛ Bruno A M P Besen3؛ Katia R M Leite1؛ Victor Srougi1؛ Miguel Srougi1، 2؛ Sabrina T Reis1، 2
1Laboratory of Medical Investigation (LIM55), Urology Department, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil
2Moriah Hospital, São Paulo, SP, Brazil
3Instituto Central, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de SaoPaulo, Sao Paulo, SP, Brazil
چکیده
COVID-19 represents a public health emergency, whose mechanism of which is not fully understood. It is speculated that microRNAs may play a crucial role in host cells after infection by SARS-CoV-2. Thus, our study aimed to analyze the expression of miR-200c-3p in saliva samples from patients with COVID-19. One handred eleven samples from patients with COVID-19 were divided into 4 groups. Group I: 39 patients negative for Covid-19; Group II: 37 positive and symptomatic patients, with no indication of hospitalization; Group III: 21 patients with respiratory disorders (hospitalized); Group IV: 14 patients with severe conditions (oxygen therapy). The expression levels of miR-200c-3p were determined using qPCR. We found greater expression of miR-200c-3p in patients in group IV (p <0.0001), and also verified that patients aged ≥42 years had a higher expression of this miR (p =0.013). Logistic regression analysis revealed that the expression of miR-200c-3p and systemic arterial hypertension are factors independently associated with patients in group IV (p <0.0001). Our results suggest that miR-200c-3p is a predictor of severity independent of COVID-19 risk factors, which could represent a way of screening patients affected by SARS-CoV-2.
کلیدواژه‌ها
microRNA؛ COVID-19؛ SARs-CoV-2؛ Biomarkers
مراجع
  1. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116:281-297.
  2. Cullen BR. Viruses and microRNAs: RISCy interactions with serious consequences. Genes Dev 2011;25:1881-1894.
  3. Majer A, Caligiuri KA, Gale KK, Niu Y, Phillipson CS, Booth TF, Booth SA. Induction of Multiple miR-200/182 Members in the Brains of Mice Are Associated with Acute Herpes Simplex Virus 1 Encephalitis. PLoS One 2017;12:e0169081.
  4. Canatan D, De Sanctis V. The impact of MicroRNAs (miRNAs) on the genotype of coronaviruses. Acta Biomed 2020;91:195-198.
  5. Tahamtan A, Inchley CS, Marzban M, Tavakoli-Yaraki M, Teymoori-Rad M, Nakstad B, Salimi V. The role of microRNAs in respiratory viral infection: friend or foe? Rev Med Virol 2016;26:389-407.
  6. Rakhmetullina A. How miRNAs can protect humans from coronaviruses COVID-19, SARS-CoV, and MERS-CoV. 2020.
  7. Guterres A, de Azeredo Lima CH, Miranda RL, Gadelha MR. What is the potential function of microRNAs as biomarkers and therapeutic targets in COVID-19? Infect Genet Evol 2020;85:104417.
  8. Report CdC-S: World Health Organization. https://www.who.int/ Accessed 04/02 2020.
  9. Coronavirus COVID-19 outbreak: Latest news, information and updates. 2020.
  10. Wu Z, McGoogan JM. Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China: Summary of a Report of 72 314 Cases From the Chinese Center for Disease Control and Prevention. JAMA 2020;323:1239-1242.
  11. Zhou F, Yu T, Du R, Fan G, Liu Y, Liu Z, Xiang J, Wang Y, Song B, Gu X, Guan L, Wei Y, Li H, Wu X, Xu J, Tu S, Zhang Y, Chen H, Cao B. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet 2020;395:1054-1062.
  12. Jin JM, Bai P, He W, Wu F, Liu XF, Han DM, Liu S, Yang JK. Gender Differences in Patients With COVID-19: Focus on Severity and Mortality. Front Public Health 2020;8:152.
  13. Buggele WA, Johnson KE, Horvath CM. Influenza A virus infection of human respiratory cells induces primary microRNA expression. J Biol Chem 2012;287:31027-31040.
  14. Momen-Heravi F, Trachtenberg AJ, Kuo WP, Cheng YS. Genomewide Study of Salivary MicroRNAs for Detection of Oral Cancer. J Dent Res 2014;93(7 Suppl):86S-93S.
  15. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25:402-408.
  16. Viana N, Pimenta R, Gonçalves GL, Gomes TF, Guimaraes VR, Sro V, Sro M, Leite K, Reis S. Hydroxychloroquine Efficacy for the Treatment of Patients with COVID-19: A Systematic Review and Meta-Analysis 2020.
  17. Schwarz DS, Hutvagner G, Du T, Xu Z, Aronin N, Zamore PD. Asymmetry in the assembly of the RNAi enzyme complex. Cell 2003;115:199-208.
  18. Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol. 2005;6:376-385.
  19. Liu Q, Du J, Yu X, Xu J, Huang F, Li X, Zhang C, Li X, Chang J, Shang D, Zhao Y, Tian M, Lu H, Xu J, Li C, Zhu H, Jin N, Jiang C. miRNA-200c-3p is crucial in acute respiratory distress syndrome. Cell Discov 2017;3:17021.
  20. Lu D, Chatterjee S, Xiao K, Riedel I, Wang Y, Foo R, Bar C, Thum T. MicroRNAs targeting the SARS-CoV-2 entry receptor ACE2 in cardiomyocytes. J Mol Cell Cardiol 2020;148:46-49.
  21. EPIDEMIA COVID-19. https://www.epice ntro.iss.it/coron aviru s/bolle ttino/ Bolle ttino -sorve glian za-integ rata-COVID -19_16-giugn o-2020.pdf Accessed Accessed.
  22. Pontecorvi G, Bellenghi M, Ortona E, Carè A. microRNAs as new possible actors in gender disparities of Covid-19 pandemic. Acta Physiol (Oxf) 2020:e13538.
  23. Czumbel LM, Kiss S, Farkas N, Mandel I, Hegyi A, Nagy Á, Lohinai Z, Szakacs Z, Hegyi P, Steward MC, Varga G. Saliva as a Candidate for COVID-19 Diagnostic Testing: A Meta-Analysis. Front Med (Lausanne) 2020;7:465.
  24. Flanagin A, Fontanarosa PB, Bauchner H. Preprints Involving Medical Research-Do the Benefits Outweigh the Challenges?. JAMA 2020;324:1840-1843.
  25. Orsi C: Na pandemia, pré-prints servem mais à retórica que ciência. https://www.revistaquestaodeciencia.com.br/index.php/artigo/2020/11/11/na-pandemia-pre-prints-servem-mais-retorica-que-ciencia (2020). Accessed 12-01-2020 2020.
آمار
تعداد مشاهده مقاله: 1,819
تعداد دریافت فایل اصل مقاله: 1,225


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب