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Virulence factors, serogroups, and antibiotic resistance of Shiga-toxin producing Escherichia coli from raw beef, chicken meat, and vegetables in Southwest Iran | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 3 - شماره پیاپی 76، 2021، صفحه 180-187 اصل مقاله (442.07 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.39266.5706 | ||
| نویسندگان | ||
| S. Kholdi1؛ M. Motamedifar* 2؛ F. Fani3؛ S. Mohebi1؛ A. Bazargani* 4 | ||
| 1Ph.D. Student in Bacteriology, Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Shiga-toxin-producing Escherichia coli (STEC) is an important food-borne pathogen causing human diseases with severe symptoms. Although the O157 serotype has been mostly isolated from human specimens, the increasing incidence rates of non-O157 serogroups have attracted special attention in recent years. Aims: Evaluation of the epidemiology and identification of different characteristics of STEC isolates from raw beef, chicken meat, and vegetable samples in Shiraz, Southwest Iran. Methods: Two hundred beef and chicken meat samples from different parts of carcasses and four hundred vegetable samples (carrots, lettuce, cucumber, and leafy greens) were randomly taken; STEC were isolated and confirmed using standard microbiological methods. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disc diffusion method. Polymerase chain reaction (PCR) was used for the identification of O-serogroups, virulence, and antibiotic resistance genes. Results: 52% of beef, 8% of chicken, and 7.2% of vegetable samples were STEC-positive. Further, the highest frequency of virulence factors belonged to the co-existence of stx1 and stx2. O157 serogroup was only detected in beef (3.8%) and lettuce (16.6%) isolates, while the rates of the non-O157 serogroups were relatively high (up to 44.2%). The highest resistance rate in the STEC isolates of different samples belonged to nalidixic acid (62.5%), tetracycline (55.7%), and ampicillin (48%). Conclusion: Paying more attention to non-O157 serogroups in future studies is recommended due to the relatively high prevalence of theses STEC serogroups in our study. Besides, the high level of resistance to some antibiotics observed in this study needs to be addressed. | ||
| کلیدواژهها | ||
| ESBL؛ Foodborne pathogen؛ O-serogroups؛ STEC؛ Virulence genes | ||
| مراجع | ||
|
Ahmed, OB and Dablool, A (2017). Quality improvement of the DNA extracted by boiling method in gram negative bacteria. Int. J. Bioassays. 6: 5347-5349. Alegría, Á; Arias-Temprano, M; Fernández-Natal, I; Rodríguez-Calleja, JM; García-López, ML and Santos, JA (2020). Molecular diversity of ESBL-producing Escherichia coli from foods of animal origin and human patients. Int. J. Environ. Res. Public Health. 17: 1312-1321. Ateba, CN and Mbewe, M (2011). Detection of Escherichia coli O157: H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: public health implications. Res. Microbiol., 162: 240-248.
Attari, VE; Abbasi, MM; Abedimanesh, N; Ostadrahimi, A and Gorbani, A (2014). Investigation of enrofloxacin and chloramphenicol residues in broiler chickens carcasses collected from local markets of Tabriz, Northwestern Iran. Health Promot. Perspect., 4: 151-157.
Balamurugan, S; Ahmed, R; Gao, A and Strange, P (2017). Comparison of the fate of the top six non-O157 shiga-toxin producing Escherichia coli (STEC) and E. coli O157: H7 during the manufacture of dry fermented sausages. Int. J. Food Microbiol., 259: 14-21. Bardasi, L; Taddei, R; Nocera, L; Ricchi, M and Merialdi, G (2015). Shiga toxin-producing Escherichia coli in meat and vegetable products in Emilia Romagna Region, years 2012-2013. Ital. J. Food Saf., 4: 33-37. Boerlin, P; McEwen, SA; Boerlin-Petzold, F; Wilson, JB; Johnson, RP and Gyles, CL (1999). Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J. Clin. Microbiol., 37: 497-503. Bonyadian, M; Moshtaghi, H and Mohamadtaghipour, L (2017). Antibiotic resistance of Verotoxigenic Escherichia coli isolated from vegetables. BJM., 5: 9-16. Cepeda-Molero, M; Berger, CN; Walsham, AD; Ellis, SJ; Wemyss-Holden, S; Schüller, S; Frankel, G and Fernández, LÁ (2017). Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors. PLoS Pathogens. 13: 257-263. Conrad, C; Stanford, K; McAllister, T; Thomas, J and Reuter, T (2016). Shiga toxin-producing Escherichia coli and current trends in diagnostics. Anim. Front., 6: 37-43. Coombes, BK; Gilmour, MW and Goodman, CD (2011). The evolution of virulence in non-O157 Shiga toxin-producing Escherichia coli. Front. Microbiol., 2: 90-94. Dehkordi, FS; Tavakoli-Far, B; Jafariaskari, S; Momtaz, H; Esmaeilzadeh, S; Ranjbar, R and Rabiei, M (2020). Uropathogenic Escherichia coli in the high vaginal swab samples of fertile and infertile women: virulence factors, O-serogroups, and phenotyping and genotyping characterization of antibiotic resistance. New Microbes and New Infect., 38: 1-11. Dulo, F (2014). Prevalence and antimicrobial resistance profile of Escherichia coli O157: H7 in goat slaughtered in dire dawa municipal abattoir as well as food safety knowledge, attitude and hygiene practice assessment among slaughter staff, Ethiopia. MSc Thesis, Addis Ababa, Ethiopia: Addis Ababa University. Dutta, T; Warjri, I; Roychoudhury, P; Lalzampuia, H; Samanta, I; Joardar, S; Bandyopadhyay, S and Chandra, R (2013). Extended-spectrum-β-lactamase-producing Escherichia coli isolate possessing the Shiga toxin gene (stx 1) belonging to the O64 serogroup associated with human disease in India. J. Clin. Microbiol., 51: 2008-2009. Ercoli, L; Farneti, S; Zicavo, A; Mencaroni, G; Blasi, G; Striano, G and Scuota, S (2016). Prevalence and characteristics of verotoxigenic Escherichia coli strains isolated from pigs and pork products in Umbria and Marche regions of Italy. Int. J. Food Microbiol., 232: 7-14. Etcheverria, AI and Padola, NL (2013). Shiga toxin-producing Escherichia coli: factors involved in virulence and cattle colonization. Virulence. 4: 366-372. Fan, R; Shao, K; Yang, X; Bai, X; Fu, S; Sun, H; Xu, Y; Wang, H; Li, Q and Hu, B (2019). High prevalence of non-O157 Shiga toxin-producing Escherichia coli in beef cattle detected by combining four selective agars. BMC Microbiol., 19: 213-219. Ferens, WA and Hovde, CJ (2011). Escherichia coli O157: H7: animal reservoir and sources of human infection. Foodborne Pathog. Dis., 8: 465-487. Franz, E; van Hoek, AH; Wuite, M; van der Wal, FJ; de Boer, AG; Bouw, E and Aarts, HJ (2015). Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC). PLoS One, 10: 511-516. Geser, N; Stephan, R and Hächler, H (2012). Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet. Res., 8: 21-26. Hassan, SA; Altalhi, AD; Gherbawy, YA and El-Deeb, BA (2011). Bacterial load of fresh vegetables and their resistance to the currently used antibiotics in Saudi Arabia. Foodborne Pathog. Dis., 8: 1011-1018. Herman, K; Hall, A and Gould, L (2015). Outbreaks attributed to fresh leafy vegetables, United States, 1973-2012. Epidemiol. Infect., 143: 3011-3021. Hessain, AM; Al-Arfaj, AA; Zakri, AM; El-Jakee, JK; Al-Zogibi, OG; Hemeg, HA and Ibrahim, IM (2015). Molecular characterization of Escherichia coli O157: H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia. Saudi J. Biol. Sci., 22: 725-729. Iweriebor, BC; Iwu, CJ; Obi, LC; Nwodo, UU and Okoh, AI (2015). Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa. BMC Microbiol., 15: 1-9. Kohansal, M and Asad, AG (2018). Molecular analysis of Shiga toxin-producing Escherichia coli O157: H7 and non-O157 strains isolated from calves. Onderstepoort J. Vet. Res., 85: 1-7. Koochakzadeh, A; Badouei, MA; Mazandarani, E and Madadgar, O (2014). Survey on O157: H7 enterohemorrhagic Escherichia coli (EHEC) in cattle in Golestan province, Iran. Iran J. Microbiol., 6: 276-282. Lavilla, S; Gonzalez-Lopez, J; Sabate, M; Garcia-Fernandez, A; Larrosa, M; Bartolome, R; Carattoli, A and Prats, G (2008). Prevalence of qnr genes among extended-spectrum β-lactamase-producing enterobacterial isolates in Barcelona, Spain. J. Antimicrob. Chemother., 61: 291-295. Leotta, GA; Miliwebsky, ES; Chinen, I; Espinosa, EM; Azzopardi, K; Tennant, SM; Robins-Browne, RM and Rivas, M (2008). Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand. BMC Microbiol., 8: 46-51. Majowicz, SE; Scallan, E; Jones-Bitton, A; Sargeant, JM; Stapleton, J; Angulo, FJ; Yeung, DH and Kirk, MD (2014). Global incidence of human Shiga toxin-producing Escherichia coli infections and deaths: a systematic review and knowledge synthesis. Foodborne Pathog. Dis., 11: 447-455.
Mandakini, R; Dutta, TK; Chingtham, S; Roychoudhury, P; Samanta, I; Joardar, SN; Pachauau, AR and Chandra, R (2015). ESBL-producing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India. Trop. Anim. Health Prod., 47: 377-381.
Manyi-Loh, C; Mamphweli, S; Meyer, E and Okoh, A (2018). Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules. 23: 795-801. Mashak, Z (2018). Virulence genes and phenotypic evaluation of the antibiotic resistance of vero toxin producing Escherichia coli recovered from milk, meat, and vegetables. Jundishapur J. Microbiol., 11: 1-8. Mathusa, EC; Chen, Y; Enache, E and Hontz, L (2010). Non-O157 Shiga toxin-producing Escherichia coli in foods. J. Food Prot., 73: 1721-1736. Melton-Celsa, AR(2014). Shiga toxin(Stx) classification, structure, and function. Microbiol. Spectr., 54: 333-337. Memariani, M; Peerayeh, SN; Salehi, TZ and Mostafavi, SKS (2015). Occurrence of SHV, TEM and CTX-M β-lactamase genes among enteropathogenic Escherichia coli strains isolated from children with diarrhea. Jundishapur J. Microbiol., 8: 1-8. Minh, DH; Minh, SH; Honjoh, KI and Miyamoto, T (2016). Isolation and bio-control of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli contamination in raw chicken meat by using lytic bacteriophages. LWT., 71: 339-346. Mohammadi-Sardo, MR; Salehi, S; Mirbaha, S and Abdollahi, A (2017). Shiga toxigenic Escherichia coli antimicrobial resistance properties in diabetic and nondiabetic pediatric patients; a case-control study. Int. J. Pediatr., 5: 5999-6008.
Momtaz, H; Dehkordi, FS; Rahimi, E; Ezadi, H and Arab, R (2013a). Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant’s meat. Meat Sci., 95: 381-388.
Momtaz, H; Karimian, A; Madani, M; Dehkordi, FS; Ranjbar, R; Sarshar, M and Souod, N (2013b). Uropathogenic Escherichia coli in Iran: serogroup distributions, virulence factors and antimicrobial resistance properties. Ann. Clin. Microbiol. Antimicrob., 12: 1-12. Mooljuntee, S; Chansiripornchai, P and Chansiripornchai, N (2010). Prevalence of the cellular and molecular antimicrobial resistance against E. coli isolated from Thai broilers. Wetchasan Sattawaphaet., 40: 311-315.
Nasrolahei, M; Zahedi, B; Bahador, A; Saghi, H; Kholdi, S; Jalalvand, N and Esmaeili, D (2014). Distribution of bla OXA-23, IS Aba, Aminoglycosides resistant genes among burned & ICU patients in Tehran and Sari, Iran. Ann. Clin. Microbiol. Antimicrob., 13: 1-4. Noguera, P; Posthuma-Trumpie, G; Van Tuil, M; Van der Wal, F; De Boer, A; Moers, A and Van Amerongen, A (2011). Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Anal. Bioanal. Chem., 399: 831-838. Ojo, O; Ajuwape, A; Otesile, E; Owoade, A; Oyekunle, M and Adetosoye, A (2010). Potentially zoonotic shiga toxin-producing Escherichia coli serogroups in the faeces and meat of food-producing animals in Ibadan, Nigeria. Int. J. Food Microbiol., 142: 214-221. Osek, J and Gallien, P (2002). Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods. Vet. Med. (Praha)., 47: 149-158. Ozpınar, H; Turan, B; Tekiner, IH; Tezmen, G; Gökçe, I Panel, EB; Koutsoumanis, K; Allende, A; Alvarez-Ordóñez, A; Bover-Cid, S; Chemaly, M; Davies, R; De Cesare, A; Herman, L and Hilbert, F (2020). Pathogenicity assessment of Shiga toxin-producing Escherichia coli (STEC) and the public health risk posed by contamination of food with STEC. EFSA J., 18: 1-8. Raeispour, M and Ranjbar, R (2018). Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains. Antimicrob. Resist. Infect. Control., 7: 1-9. Ranjbar, R; Dehkordi, FS; Shahreza, MHS and Rahimi, E (2018). Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products. Antimicrob. Resist. Infect. Control., 7: 53-59. Ranjbar, R; Masoudimanesh, M; Dehkordi, FS; Jonaidi-Jafari, N and Rahimi, E (2017a). Shiga (Vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties. Antimicrob. Resist. Infect. Control., 6: 4-8. Ranjbar, R; Seif, A and Safarpoor Dehkordi, F (2019). Prevalence of antibiotic resistance and distribution of virulence factors in the shiga toxigenic Escherichia coli recovered from hospital food. Jundishapur J. Microbiol., 12: 1-8. Ranjbar, R; Sheikhshahrokh, A and Jonaidi Jafari, N (2017b). Shiga (vero) toxin producing Escherichia coli in various types of food stuffs; virulence factors, O-serogroups and antimicrobial resistance properties. J. Food Saf., 37: 1-7. Schwaiger, K; Helmke, K; Hölzel, CS and Bauer, J (2011). Antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket). Int. J. Food Microbiol., 148: 191-196. Stuart, JC; van den Munckhof, T; Voets, G; Scharringa, J; Fluit, A and Leverstein-Van Hall, M (2012). Comparison of ESBL contamination in organic and conventional retail chicken meat. Int. J. Food Microbiol., 154: 212-214. Tzipori, S; Sheoran, A; Akiyoshi, D; Donohue-Rolfe, A and Trachtman, H (2004). Antibody therapy in the management of Shiga toxin-induced hemolytic uremic syndrome. Clin. Microbiol. Rev., 17: 926-941. Zahraei Salehi, T; Safarchhi, A and Rabbani Khorrasgani, M (2006). Identification of virulence genes in isolated Escherichia coli from diarrheic calves and lambs by multiplex polymerase chain reaction. Pak. J. Biol. Sci., 9: 191-196. | ||
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