| تعداد نشریات | 24 |
| تعداد شمارهها | 886 |
| تعداد مقالات | 7,900 |
| تعداد مشاهده مقاله | 14,905,374 |
| تعداد دریافت فایل اصل مقاله | 12,668,142 |
Antibiotic sensitivity patterns in Staphylococcus spp. isolated from goat milk in association with molecular detection of antibiotic resistance genes | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 3 - شماره پیاپی 76، 2021، صفحه 239-243 اصل مقاله (303.96 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.38465.5599 | ||
| نویسندگان | ||
| A. Quraishi1؛ P. Kaur* 2؛ N. Singh Sharma2؛ A. K. Arora2 | ||
| 1MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| 2Department of Veterinary Microbiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India | ||
| چکیده | ||
| Background: Microbes become unresponsive to the drugs used to treat infections due to their ability to acquire antimicrobial resistance. Aims: The present research aimed to study the patterns of phenotypic and genotypic antimicrobial resistance in Staphylococcus spp. isolated from goat milk. Methods: A total of 200 milk samples were collected to isolate Staphylococcus spp. from mastitic and healthy goats from Punjab state, India. The isolates were then identified biochemically and molecularly (polymerase chain reaction (PCR)). An antibiotic sensitivity test was conducted using 15 different antibiotics. Molecular detection of antibiotic resistance genes was done using PCR. Chi-square test was done to study the association between genotypic and phenotypic resistance patterns among the isolates. Results: A total of 47 Staphylococcus spp. were isolated of which 33 and 14 isolates were respectively coagulase positive and negative. The isolates phenotypically showed the highest resistance to Penicillin G (P) (91.4%). Methicillin resistant Staphylococcus aureus (MRSA) was found 56.25%. Amongst the antibiotic resistance genes, tetK (87.23%) was the most prevalent isolated gene followed by blaZ (85.10%), mecA (48.93%), and tetM (14.89%). Statistical analysis revealed that the genotypic and phenotypic resistance patterns were significantly associated with penicillin and methicillin (MET) resistances. Conclusion: The high prevalence of antibiotic-resistant Staphylococcus spp., especially MRSA, in goat milk is of concern and needs to be addressed in this area. | ||
| کلیدواژهها | ||
| Antibiotic resistance؛ Antibiotic resistance genes؛ Goat؛ PCR؛ Staphylococcus spp | ||
| مراجع | ||
|
Arora, S; Agarwal, RK and Bist, B (2006). Comparison of ELISA and PCR vis-à-vis cultural methods for detecting Aeromonas spp. in foods of animal origin. Int. J. Food Microbiol., 106: 177-183.
Bergonier, D; De Crémoux, R; Rupp, R; Lagriffoul, G and Berthelot, X (2003). Mastitis of dairy small ruminants. Vet. Res., 34: 689-716.
Brown, DFJ and Reynolds, PE (1980). Intrinsic resistance to beta-lactam antibiotics in S. aureus. FEBS Lett., 122: 275-278.
Choi, SM; Kim, SH; Kim, HJ; Lee, DG; Choi, JH; Yoo, JH; Kang, JH; Shin, WS and Kang, MW (2003). Multiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species. J. Korean Med. Sci., 18: 631-636.
Emaneini, M; Bigverdi, R; Kalantar, D; Soroush, S; Jabalameli, F; Noorazar, KB; Asadollahi, P and Taherikalani, M (2013). Distribution of genes encoding tetracycline resistance and aminoglycoside modifying enzymes in S. aureus strains isolated from a burn center. Ann. Burns Fire Disasters. 26: 76-80.
Franca, CA; Peixoto, RM; Cavalcante, MB; Melo, NF; Oliveira, CJB; Veschi, JLA; Mota, RA and Costa, MM (2012). Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil. Pesq. Vet. Bras., 32: 747-753.
Georgopapadakou, NH (1993). Penicillin-binding proteins and bacterial resistance to β lactams. Antimicrob. Agents Chemother., 37: 2045-2053.
Gundogan, N; Citak, S and Turan, E (2006). Slime production, DNAse activity and antibiotic resistance of Staphylococcus aureus isolated from raw milk, pasteurized milk and ice cream samples. Food Control. 17: 389-392.
Huys, G; D’Haene, K; Van Eldere, J; Von Holy, A and Swings, J (2005). Molecular diversity and characterization of tetracyclin-resistant S. aureus isolates from a poultry processing plant. Appl. Environ. Microbiol., 71: 574-579.
Kour, G; Chandra, M; Kaur, G; Narang, D; Gupta, DK; Arora, AK and Sharma, NS (2017). Prevalence of mastitis causing organism and their antibiotic resistance pattern in dairy farms. Indian J. Dairy Sci., 70: 587-592. Martineau, F; Picard, JF; Lansac, N; Menard, C; Roy, HP; Ouellette, M and Bergeron, MG (2000). Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of S. aureus and S. epidermidis. Antimicrob. Agents Chemother., 44: 231-238.
Moroni, P; Vellere, F; Antonini, M; Pisoni, G; Ruffo, G and Carli, S (2004). Antibiotic susceptibility of coagulase-negative staphylococci isolated from goats’ milk. Int. J. Antimicrob. Agents., 23: 637-640.
Poutrel, B; De Cremoux, R; Ducelliez, M and Verneau, D (1997). Control of intramammary infections in goats: impact on somatic cell counts. Anim. Sci. J., 75: 566-570.
Rahmdel, S; Hosseinzadeh, S; Shekarforoush, SS; Torriani, S; Gatto, V and Pashangeh, S (2018). Safety hazards in bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. Microb. Pathog., 116: 100-108.
Rola, JG; Sosnowski, M; Ostrowska, M and Osek, J (2015). Prevalence and antimicrobial resistance of coagulase-positive staphylococci isolated from raw goat milk. Small Rumin. Res., 123: 124-128.
Ruiz, P; Barragán, I; Seseña, S and Palop, ML (2016). Is staphylococci population from milk of healthy goats safe? Int. J. Food Microbiol., 238: 146-152.
Sabath, LD (1979). Staphylococcal tolerance to penicillins and cephalosporins. ASM., PP: 299-303.
Sabath, LD (1982). Mechanisms of resistance to β-lactam antibiotics in strains of S. aureus. Ann. Intern. Med., 97: 339-344.
Santis, EPL; Mencarelli, A and Nieddu, MP (2001). Efficacy of endomammarian administration of cloxacillin benzatine for the treatment of intramammary infections in the dry ewes. Large Anim. Rev., 7: 39-47.
Sarangi, LN; Panda, HK; Priyadarshini, A; Palai, T; Ranabijuli, S; Sahoo, S; Dash, AK; Mohanty, N; Kar, BC and Mohanty, DN (2009). Antibiogram and drug resistance of Staphylococcus aureus isolated from bovine clinical and subclinical mastitis. J. Res., 27: 136-138.
Sree, PD and Ayodha, S (2016). Bacteriological and antibiogram studies of milk samples of clinical mastitis in goats. IOSR-JAVS., 9: 33-35.
Strommenger, B; Kettlitz, C; Werner, G and Witte, W (2003). Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in S. aureus. J. Clin. Microbiol., 41: 4089-4094.
Ünal, N; Askar, S; Macun, HC; Sakarya, F; Altun, B and Yıldırım, M (2012). Panton-Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants. Trop. Anim. Health Prod., 44: 573-579.
Virdis, S; Scarano, C; Cossu, F; Spanu, V; Spanu, C and De Santis, EPL (2010). Antibiotic resistance in Staphylococcus aureus and coagulase negative staphylococci isolated from goats with subclinical mastitis. Vet. Med. Int., 2010: 517060. doi: 10.4061/2010/517060.
Walther, B; Friedrich, AW; Brunnberg, L; Wieler, LH and Becker, LA (2006). Methicillin resistant S. aureus (MRSA) in veterinary medicine: a new emerging pathogen? Berl. Munch. Tierarztl. Wochenschr., 119: 222-232.
WHO (2001). Global strategy for containment of antimicrobial resistance. http://www.who.int/csr/resources/publications/ drugresist/WHO_CDS_CSR_DRS_2001_2_EN/en/. | ||
|
آمار تعداد مشاهده مقاله: 986 تعداد دریافت فایل اصل مقاله: 652 |
||