| تعداد نشریات | 24 |
| تعداد شمارهها | 912 |
| تعداد مقالات | 8,158 |
| تعداد مشاهده مقاله | 15,836,160 |
| تعداد دریافت فایل اصل مقاله | 13,332,069 |
Deterioration of wound healing and intense suppression of MMP-9 mRNA expression after short-term administration of different topical glucocorticoids or NSAIDs in an avian model of corneal lesions | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 3 - شماره پیاپی 76، 2021، صفحه 188-194 اصل مقاله (393.48 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.39864.5783 | ||
| نویسندگان | ||
| H. Mirarab Razi1؛ N. Mosleh2؛ T. Shomali* 3؛ N. Tavangar1؛ F. Namazi4 | ||
| 1Resident of Avian Medicine, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 3Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran; 4Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 4Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Background: Corneal lesions are considered among important ophthalmic conditions in avian patients. Short-term outcome of using anti-inflammatory agents in corneal lesions of birds are not well-described. Aims: The study evaluates effects of different anti-inflammatory agents on healing of alkali burn-induced corneal lesions in layer hens as an avian model. Methods: Adult layers were randomly allocated into 7 groups (n=15) as follows: 1. Negative (normal) control (NC), and 2. Positive control (PC) with an experimentally induced-corneal lesion, 3-7. Birds with corneal lesions that were treated with dexamethasone, fluorometholone, prednisolone, ketorolac, or diclofenac eye-drops every 6 hours (QID) for 5 consecutive days. Results: At the end of the experiment, proper healing was observed in PC group based on lesion area, while treated groups showed statistically larger lesion sizes as compared to PC birds (p <0.05). Although no significant difference was observed among groups, birds treated with ketorolac, diclofenac or fluorometholone had higher histopathological scores for most of the assayed parameters than other groups. Levels of tumor necrosis factor-α (TNF-α) and vascular endothelial growth factor (VEGF) in corneal tissue of different groups were statistically the same. The mRNA expression of matrix metalloproteinase-9 (MMP-9) was increased 2.5 folds in PC group as compared to NC birds. However, birds treated with anti-inflammatory agents showed no detectable expression of MMP-9 mRNA. Conclusion: Five days of topical administration of non-steroidal anti-inflammatory agents (NSAIDs) or glucocorticoids (GCs) is associated with suppression of MMP-9 mRNA expression in corneal tissue and detrimental effects on wound healing in layers with alkali burn-induced corneal ulcers. | ||
| کلیدواژهها | ||
| Anti-inflammatory agents؛ Bird؛ Cornea؛ Healing؛ Matrix metalloproteinase-9 | ||
| مراجع | ||
|
Anderson, C; Zhou, Q and Wang, S (2014). An alkali-burn injury model of corneal neovascularization in the mouse. J. Vis. Exp., 86: e51159.
Araki-Sasaki, K; Katsuta, O; Mano, H; Nagano, T and Nakamura, M (2016). The effects of oral and topical corticosteroid in rabbit corneas. BMC Ophthalmol., 16: 1-6.
Bai, JQ; Qin, HF and Zhao, SH (2016). Research on mouse model of grade II corneal alkali burn. Int. J. Ophthalmol., 9: 487-490.
Bian, F; Shin, CS; Wang, C; Pflugfelder, SC; Acharya, G and De Paiva, CS (2016). Dexamethasone drug eluting nanowafers control inflammation in alkali-burned corneas associated with dry eye. Invest. Ophthalmol. Vis. Sci., 57: 3222-3230.
Cade, F; Paschalis, EI; Regatieri, CV; Vavvas, DG; Dana, R and Dohlman, CH (2014). Alkali burn to the eye: protection using TNF-α inhibition. Cornea. 33: 382-389.
Calder, LA; Balasubramanian, S and Fergusson, D (2005). Topical nonsteroidal anti-inflammatory drugs for corneal abrasions: meta-analysis of randomized trials. Acad. Emerg. Med., 12: 467-473.
Carvalho, CMD; Rodarte-Almeida, ACDV; Santana, MIS and Galera, PD (2018). Avian ophthalmic peculiarities. Ciência Rural. 48: e20170904.
Castro, MR; Lutz, D and Edelman, JL (2004). Effect of COX inhibitors on VEGF-induced retinal vascular leakage and experimental corneal and choroidal neovascularization. Exp. Eye Res., 79: 275-285.
De Paiva, CS; Corrales, RM; Villarreal, AL; Farley, WJ; Li, DQ; Stern, ME and Pflugfelder, SC (2006). Corticosteroid and doxycycline suppress MMP-9 and inflammatory cytokine expression, MAPK activation in the corneal epithelium in experimental dry eye. Exp. Eye Res., 83: 526-535.
Ferrari, G; Bignami, F and Rama, P (2015). Tumor necrosis factor-α inhibitors as a treatment of corneal hemangiogenesis and lymphangiogenesis. Eye Contact Lens. 41: 72-76.
Gao, M; Sang, W; Liu, F; Yu, H; Zhou, R; Belin, MW; Zloty, P and Chen, Y (2016). High MMP-9 expression may contribute to retroprosthetic membrane formation after KPro implantation in rabbit corneal alkali burn model. J. Ophthalmol., 2016: Article ID:1094279.
Griffith, GL; Wirostko, B; Lee, HK; Cornell, LE; McDaniel, JS; Zamora, DO and Johnson, AJ (2018). Treatment of corneal chemical alkali burns with a crosslinked thiolated hyaluronic acid film. Burns. 44: 1179-1186.
Iwamoto, S; Koga, T; Ohba, M; Okuno, T; Koike, M; Murakami, A; Matsuda, A and Yokomizo, T (2017). Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B 4 receptor 2. Sci. Rep., 7: 1-10.
Kadmiel, M; Janoshazi, A; Xu, X and Cidlowski, JA (2016). Glucocorticoid action in human corneal epithelial cells establishes roles for corticosteroids in wound healing and barrier function of the eye. Exp Eye Res., 152: 10-33.
Kaya, SG; Inanc-Surer, S; Cakan-Akdogan, G; Oktay, G, Utine, CA and Kalyoncu, S (2021). Roles of matrix metalloproteinases in cornea: a special focus on macular corneal dystrophy. Med. Drug Discov. [In press]. doi: https://doi.org/10.1016/j.medidd.2021.100095.
Li, H; Fan, TJ; Zou, P and Xu, B (2021). Diclofenac sodium triggers p53-dependent apoptosis in human corneal epithelial cells via ROS-mediated crosstalk. Chem. Res. Toxicol., 34: 70-79.
Li, ZR; Li, YP; Lin, ML; Su, WR; Zhang, WX; Zhang, Y; Yao, L and Liang, D (2012). Activated macrophages induce neovascularization through upregulation of MMP-9 and VEGF in rat corneas. Cornea. 31: 1028-1035.
Li, DQ; Lokeshwar, BL; Solomon, A; Monroy, D; Ji, Z and Pflugfelder, SC (2001). Regulation of MMP-9 production by human corneal epithelial cells. Exp. Eye Res., 73: 449-459. doi: 10.1006/exer.2001.1054. PMID: 11825017.
Mulholland, B; Tuft, SJ and Khaw, PT (2005). Matrix metalloproteinase distribution during early corneal wound healing. Eye. 19: 584-588.
Nyati, KK; Prasad, KN; Agrawal, V and Husain, N (2017). Matrix metalloproteinases-2 and -9 in Campylobacter jejuni-induced paralytic neuropathy resembling Guillain-Barré syndrome in chickens. Microb. Pathog., 111: 395-401.
OECD (2016). ANNEX II: Atlas of histopathological lesions of isolated chicken eyes. Addendum to Draft Revised GD 160 v. 21 Dec. 2016. https://www.oecd.org/env/ehs/testing/ AnnexII_DraftRevisedGD160_21Dec2016_compressed_images.pdf.
Pfaffl, MW (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res., 29: e45.
Philipp, W; Speicher, L and Humpel, C (2000). Expression of vascular endothelial growth factor and its receptors in inflamed and vascularized human corneas. Invest. Ophthalmol. Vis. Sci., 41: 2514-2522. Reviglio, VE; Rana, TS; Li, QJ; Ashraf, MF; Daly, MK and O’Brien, TP (2003). Effects of topical nonsteroidal antiinflammatory drugs on the expression of matrix metalloproteinases in the cornea. J. Cataract Refract Surg., 29: 989-997.
Ryu, JS; Ko, JH; Kim, MK; Wee, WR and Oh, JY (2017). Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci. Rep., 7: 4135.
Singh, A; Maurya, OP; Jagannadhan, MV and Patel, A (2012). Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography. J. Ocul. Biol. Dis. Infor., 5: 31-35.
Smith, CH and Goldman, RD (2012). Topical nonsteroidal anti-inflammatory drugs for corneal abrasions in children. Can. Fam. Physician. 58: 748-749.
Srinivasan, M; Mascarenhas, J; Rajaraman, R; Ravindran, M; Lalitha, P; Ray, KJ; Zegans, ME; Acharya, NR; Lietman, TM and Keenan, JD (2014). Steroids for corneal ulcers trial group. Visual recovery in treated bacterial keratitis. Ophthalmology. 121: 1310-1311.
Steer, JH; Kroeger, KM; Abraham, LJ and Joyce, DA (2000). Glucocorticoids suppress tumor necrosis factor-alpha expression by human monocytic THP-1 cells by suppressing transactivation through adjacent NF-kappa B and c-Jun-activating transcription factor-2 binding sites in the promoter. J. Biol. Chem., 275: 18432-18440.
Stevenson, W; Cheng, SF; Dastjerdi, MH; Ferrari, G and Dana, R (2012). Corneal neovascularization and the utility of topical VEGF inhibition: ranibizumab (Lucentis) vs bevacizumab (Avastin). Ocul. Surf., 10: 67-83.
Thiel, B; Sarau, A and Ng, D (2017). Efficacy of topical analgesics in pain control for corneal abrasions: a systematic review. Cureus. 9: e1121.
Williams, D (1994). Ophthalmology. In: Ritchie, BW; Harrison, GJ and Harrison, LR (Eds.), Avian medicine: principles and application. Florida, USA, Wingers Publishing. PP: 673-694. | ||
|
آمار تعداد مشاهده مقاله: 1,008 تعداد دریافت فایل اصل مقاله: 849 |
||