اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها851
تعداد مقالات7,575
تعداد مشاهده مقاله13,413,813
تعداد دریافت فایل اصل مقاله11,580,480
Nguyen, Thanh-Tan, Vo-Nguyen, Hai-Vy, Tran-Van, Hieu. (1400). Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool. , 10(3), 105-108. doi: 10.22099/mbrc.2021.39728.1588
Thanh-Tan Nguyen; Hai-Vy Vo-Nguyen; Hieu Tran-Van. "Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool". , 10, 3, 1400, 105-108. doi: 10.22099/mbrc.2021.39728.1588
Nguyen, Thanh-Tan, Vo-Nguyen, Hai-Vy, Tran-Van, Hieu. (1400). 'Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool', , 10(3), pp. 105-108. doi: 10.22099/mbrc.2021.39728.1588
Nguyen, Thanh-Tan, Vo-Nguyen, Hai-Vy, Tran-Van, Hieu. Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool. , 1400; 10(3): 105-108. doi: 10.22099/mbrc.2021.39728.1588

Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool

Molecular Biology Research Communications
دوره 10، شماره 3، 2021، صفحه 105-108    اصل مقاله (249.53 K)
نوع مقاله: Short communication
شناسه دیجیتال (DOI): 10.22099/mbrc.2021.39728.1588
نویسندگان
Thanh-Tan Nguyen1، 2؛ Hai-Vy Vo-Nguyen1، 2؛ Hieu Tran-Van* 1، 2
1Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam
2Vietnam National University, Ho Chi Minh City, Vietnam
چکیده
GFP is an old-yet-powerful protein marker, which has been widely used in molecular biotechnology due to its capacity of exhibiting bright green fluorescence when exposed to ultraviolet light. The hFc region of IgG antibodies is a specific binding ligand of expressed receptors on immune cells with well-known cellular-associated functions like opsonization and phagocytosis. In this present study, we proceeded to fuse gfp-hfc gene into pET-28a to create a recombinant pET-28a-gfp-hfc vector. The expression of GPF-hFc was induced by IPTG and confirmed using SDS-PAGE and followed by Western blot probed with 6xHis antibodies. This chimeric protein was utilized in specific binding experiments with protein A/G-coated magnetic beads using a fluorescence microscope. Due to its fluorescence and binding ability, GFP-hFc could be used as a model molecule for monitoring molecule detection studies, tracking nanoparticle migration and distribution, or stimulating immune responses.
کلیدواژه‌ها
GFP؛ hFc-tag؛ GPF-hFc؛ protein A/G-coated magnetic beads
مراجع
  1. Hori Y. Crystal structure of the Aequorea victoria green fluorescent protein. Tanpakushitsu Kakusan Koso 2007;52:1768-1769.
  2. An NH, Truong DT, Hieu TV. Cloning, expression and purification of m-cell specific binding peptide (CO1) fused with GFP. Vietnam J Biotechnol 2016;14:599-604.
  3. Shukla AA, Gupta P, Han X. Protein aggregation kinetics during Protein A chromatography. Case study for an Fc fusion protein. J Chromatogr A 2007;1171:22-28.
  4. Stripecke R, del Carmen Villacres M, Skelton DC, Satake N, Halene S, Kohn DB. Immune response to green fluorescent protein: implications for gene therapy. Gene Ther 1999;6: 1305-1312.
  5. Manat Y, Shustov AV, Evtehova E, Eskendirova SZ. Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species. Open Vet J 2016;6:71-77.
  6. Xu T, Lin Z, Wang Ch, Li Y, Zhao M, Hua L, Zhu B. Prokaryotic expression and identification of scavenger receptor B2. Acta Virol 2018;62:50-57.
  7. Vo-Nguyen HV, Nguyen TT, Vu HTT, Nguyen TTT, Hoang QC, Tran TL, Tran-Van H. Recombinant human SCARB2 expressed in Escherichia coli and its potential in enterovirus 71 blockage. Iran J Sci Technol Trans Sci 2021;45:455-461.
  8. Robinson MP, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colussi PA, Noren CJ, Taron CH, DeLisa MP, Berkmen M. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 2015;1-9.
  9. Chan CEZ, Lim APC, Chan AHY, MacAry PA, Hanson BJ. Optimized expression of full-length IgG1 antibody in a common E.coli strain. PLos One 2010;4:e10261.
  10. Sarker A, Rathore AS, Gupta RD. Evaluation of scFv protein recovery from E.coli by in vitro refolding and mild solubilization process. Micro Cell Fac 2019;18:5:2-12.
آمار
تعداد مشاهده مقاله: 1,648
تعداد دریافت فایل اصل مقاله: 932


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب