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The effect of adding pyridoxine to soybean lecithin-based extender on goat semen quality parameters after the freeze-thawing process | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 3 - شماره پیاپی 76، 2021، صفحه 234-238 اصل مقاله (306.38 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.39828.5777 | ||
| نویسندگان | ||
| Z. Rezaei1؛ S. Zeynodini2؛ H. Baghshahi* 3؛ A. Towhidi2؛ S. Faraji4 | ||
| 1MSc in Animal physiology, Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Graduated from Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 4Graduated form Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده | ||
| Background: Supplementing the semen extender with various antioxidants can increase the quality of semen. Aims: The aim of the present study was to investigate the addition of pyridoxine (vitamin B6), a phenolic compound with antioxidant properties, to soybean lecithin extender on motility and quality indices of goat sperm after freezing-thawing process. Methods: Semen was collected at weekly intervals from four Mahabadi goats, mixed, and was divided into 7 subsamples. They were then diluted with the basal extender supplemented with 0, 2, 4, 6, 8, 10, and 12 mM pyridoxine. Following freeze-thawing process, quality parameters such as sperm motility characteristics, viability, plasma membrane integrity, and malondialdehyde content were determined. Results: The results showed that pyridoxine at the level of 6 mM caused the highest total motility rate (P<0.05). Progressive sperm motility was highest at the 4, 6, and 8 mM pyridoxine (P<0.05). Although the control group showed the least progressive motility, it was not statistically significantly different from the 12 mM level. Among the pyridoxine levels, the 6 mM level recorded the best performance in term of plasma membrane integrity, sperm viability, and decreasing malondialdehyde concentration compared to the control group (P<0.05). Conclusion: The findings indicated that soybean lecithin extender supplemented with 6 mM pyridoxine can improve motility and quality parameters such as viability, plasma membrane integrity, and reduce oxidative stress of goat sperm after thawing. | ||
| کلیدواژهها | ||
| Antioxidant؛ Malondialdehyde؛ Soybean lecithin extender؛ Sperm؛ Vitamin B6 | ||
| مراجع | ||
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