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The effect of adding pyridoxine to soybean lecithin-based extender on goat semen quality parameters after the freeze-thawing process | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 3 - شماره پیاپی 76، 2021، صفحه 234-238 اصل مقاله (306.38 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2021.39828.5777 | ||
| نویسندگان | ||
| Z. Rezaei1؛ S. Zeynodini2؛ H. Baghshahi* 3؛ A. Towhidi2؛ S. Faraji4 | ||
| 1MSc in Animal physiology, Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Graduated from Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 4Graduated form Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده | ||
| Background: Supplementing the semen extender with various antioxidants can increase the quality of semen. Aims: The aim of the present study was to investigate the addition of pyridoxine (vitamin B6), a phenolic compound with antioxidant properties, to soybean lecithin extender on motility and quality indices of goat sperm after freezing-thawing process. Methods: Semen was collected at weekly intervals from four Mahabadi goats, mixed, and was divided into 7 subsamples. They were then diluted with the basal extender supplemented with 0, 2, 4, 6, 8, 10, and 12 mM pyridoxine. Following freeze-thawing process, quality parameters such as sperm motility characteristics, viability, plasma membrane integrity, and malondialdehyde content were determined. Results: The results showed that pyridoxine at the level of 6 mM caused the highest total motility rate (P<0.05). Progressive sperm motility was highest at the 4, 6, and 8 mM pyridoxine (P<0.05). Although the control group showed the least progressive motility, it was not statistically significantly different from the 12 mM level. Among the pyridoxine levels, the 6 mM level recorded the best performance in term of plasma membrane integrity, sperm viability, and decreasing malondialdehyde concentration compared to the control group (P<0.05). Conclusion: The findings indicated that soybean lecithin extender supplemented with 6 mM pyridoxine can improve motility and quality parameters such as viability, plasma membrane integrity, and reduce oxidative stress of goat sperm after thawing. | ||
| کلیدواژهها | ||
| Antioxidant؛ Malondialdehyde؛ Soybean lecithin extender؛ Sperm؛ Vitamin B6 | ||
| مراجع | ||
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Anger, JT; Gilbert, BR and Goldstein, M (2003). Cryopreservation of sperm: indications, methods and results. J. Urol., 170: 1079-1084. Anghel, AH; Zamfirescu, S; Coprean, D and Nadolu, D (2010). Antioxidant additives effect on cytological parameters of refrigerated ram semen. Archive. 53: 294-298. Atessahin, A; Bucak, MN; Tuncer, PB and Kızıl, M (2008). Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rumin. Res., 77: 38-44. Baghshahi, H; Riasi, A; Mahdavi, AH and Shirazi, A (2014). Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation. Cryobiology. 69: 482-487. Banihani, SA (2017). A systematic review evaluating the effect of vitamin B6 on semen quality. J. Urol., 15: 1-5. Björndahl, L; Söderlund, I and Kvist, U (2003). Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment. Hum. Reprod., 18: 813-816. Bowling, FG (2011). Pyridoxine supply in human development. Semin. Cell Dev. Biol., 22: 611-618. Bucak, MN; Sarıözkan, S; Tuncer, PB; Ulutaş, PA and Akçadağ, Hİ (2009). Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryo-preservation. Small Rumin. Res., 81: 90-95. Daramola, JO; Adekunle, EO; Iyasere, OS; Oke, OE; Sorongbe, TA; Iyanda, OA; Kehinde, AR; Aluko, SP; Olaoye, IO; Gbadebo, OE and Falolu, LI (2016). Effects of coconut milk alone or supplementation with pyridoxine in tris-extenders on viability of buck spermatozoa during vitrification. Small Rumin. Res., 136: 208-213. Daramola, JO; Adekunle, EO; Oke, OE; Onagbesan, OM; Iyasere, OS; Williams, TJ; James, IJ; Oyewusi, IK and Oyewusi, JA (2015). Effects of pyridoxine supple-mentation or in combination with other antioxidants on motility, in vitro capacitation and acrosome reaction of goat buck spermatozoa during cryopreservation. Small Rumin. Res., 131: 113-117. Daramola, JO; Adekunle, EO; Oke, OE; Onagbesan, OM; Williams, TJ; Iyasere, OS; James, IJ; Oyewusi, IK and Oyewusi, JA (2017). Effects of pyridoxine in combination with different antioxidants on viability and oxidative stress parameters of cryopreserved goat buck semen. Arch. Zootec., 66: 15-21. Ehrenshaft, M; Bilski, P; Li, MY; Chignell, CF and Daub, ME (1999). A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis. Proc. Natl. Acad. Sci., 96: 9374-9378. Eidan, SM (2016). Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender. Anim. Reprod. Sci., 167: 1-7. Evans, G and Maxwell, WC (1987). Salamons’ artificial insemination of sheep and goats. 2th Edn., Butterworths, Sydney, Australia. P: 194. Holt, W (2000). Basic aspects of frozen storage of semen. Anim. Reprod. Sci., 62: 3-22. Ide, Y; Kaido, M and Koide, O (1992). Changes in spermatozoa due to large doses of pyridoxine (vitamin B6). Pathol. Int., 42: 861-869. Kaido, M; Mori, K; Ide, Y; Inoue, N and Koide, O (1991). Testicular damage by high doses of vitamin B6 (pyridoxine) in rats: a light and electron microscopical study. Exp. Mol. Pathol., 55: 63-82. Kannan, K and Jain, SK (2004). Effect of vitamin B6 on oxygen radicals, mitochondrial membrane potential, and lipid peroxidation in H2O2-treated U937 monocytes. Free Radic. Biol. Med., 36: 423-428. Koppers, AJ; Mitchell, LA; Wang, P; Lin, M and Aitken, RJ (2011). Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa. Biochem. J., 436: 687-698. Leboeuf, B; Restall, B and Salamon, S (2000). Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci., 62: 113-141. Mahfouz, MM; Zhou, SQ and Kummerow, FA (2009). Vitamin B6 compounds are capable of reducing the superoxide radical and lipid peroxide levels induced by H2O2 in vascular endothelial cells in culture. Int. J. Vitam. Nutr. Res., 79: 218-229. Mori, K; Kaido, M; Fujishiro, K; Inoue, N and Koide, O (1992). Effects of megadoses of pyridoxine on spermatogenesis and male reproductive organs in rats. Arch. Toxicol., 66: 198-203. Placer, ZA; Cushman, LL and Johnson, BC (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal. Biochem., 16: 359-364. Purdy, P (2006). A review on goat sperm cryopreservation. Small Rumin. Res., 63: 215-225. Ramis, MR; Esteban, S; Miralles, A; Tan, DX and Reiter, RJ (2015). Protective effects of melatonin and mitochondria-targeted antioxidants against oxidative stress: a review. Curr. Med. Chem., 22: 2690-2711. Ramis, MR; Esteban, S; Miralles, A; Tan, DX and Reiter, RJ (2015). Protective effects of melatonin and mitochondria-targeted antioxidants against oxidative stress: a review. Curr. Med. Chem., 22: 2690-2711. Tunali, S (2014). The effects of vitamin B6 on lens antioxidant system in valproic acid-administered rats. Hum. Exp. Toxicol., 33: 623-628. Vidal, AH; Batista, AM; Cordeiro, E; Silva, B; Gomes, WA; Pelinica, MA; Silva, SV; Medalena, M and Guerra, P (2013). Soybean lecithin-based extender as an alternative for goat sperm cryopreservation. Small Rumin. Res., 109: 47-51. | ||
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