| تعداد نشریات | 24 |
| تعداد شمارهها | 903 |
| تعداد مقالات | 8,081 |
| تعداد مشاهده مقاله | 15,203,651 |
| تعداد دریافت فایل اصل مقاله | 12,848,022 |
Enhanced inulin production by hairy root cultures of Cichorium intybus in response to Pi and Fe starvation | ||
| Molecular Biology Research Communications | ||
| دوره 10، شماره 2، 2021، صفحه 85-91 اصل مقاله (355.79 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2021.38031.1527 | ||
| نویسندگان | ||
| Somayeh Tabatabaee1؛ Forough Sanjarian* 1؛ Tahmineh Lohrasebi1؛ Mahsan Karimi2 | ||
| 1Department of Plant Bioproducts, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran | ||
| 2Entomology, Plant Pathology and Nematology Department, University of Idaho, Moscow, ID 83844-2329, USA | ||
| چکیده | ||
| Cichorium intybus is rich in inulin and has several pharmacological applications. Hairy roots culture is a valuable biotechnological tool used to produce plant secondary metabolites. Agrobacterium rhizogenes-mediated genetic transformation of chicory to hairy roots was investigated using Agrobacterium Strains A4, A13, A7, and ATCC15834. Several hairy roots were tested, from which 17 lines were selected based on their fast-growing characteristics. Results of PCR analysis revealed foreign DNA integration into the selected transgenic hairy root lines. Finally, four Adventitious roots that contained the highest ratio of total sugar to total weight (µg/gr DW), were selected. This study investigated the effects of various levels of minerals and sucrose on the production of inulin in Cichorium hairy root culture. Different levels of sucrose, phosphate (Pi) and Iron (Fe) were evaluated, separately. It was found that an increase in sucrose levels from 3 to 5% could decrease the root growth; however, 60 g/l sucrose remarkably enhanced the inulin production rate in all the examined lines. The highest biomass was achieved by applying 3.75 mM Pi but it ended in the decreasing the inulin content per unit weight. In contrast, the highest inulin accumulation and the lowest amount of biomass were observed in 0.5 mM Pi. Fe starvation caused the biomass decrease and a significant increase in inulin accumulation. Results of this study suggest a successfully optimized culture medium to initiate the induction of Cichorium intybus hairy root cells to produce inulin as a valuable medicinal secondary metabolite. | ||
| کلیدواژهها | ||
| Cichorium intybus؛ Inulin؛ Agrobacterium rhizogenes؛ Phosphate starvation؛ Iron starvation | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
1. Satmbekova D, Srivedavyasasri R. Orazbekov Y, Omarova R, Datkhayev U, Ross SA. Chemical and biological studies on Cichorium intybus L. Nat Prod Res 2018;32:1343-1347.
2. Saeed M, El-Hack ME, Alagawany M, Arian MA, Arif M, Mirza MA, Naveed M, Chao S, Sarwar M, Sayab M, Dhama K. Chicory (cichorium intybus) herb: Chemical composition, pharmacology, nutritional and healthical applications. Inter J Pharmacol 2017;13:351-360.
3. Zaman R, Basar SN. A review article of Beekhe Kasni (Cichorium intybus) its traditional uses and pharmacological actions. Res J Pharmaceutical Sci 2013;2:1-4.
4. Mensink MA, Frijlink HW, Maarschalk KVDV, Hinrichs WLJ. Inulin, a flexible oligosaccharide I: Review of its physicochemical characteristics. Carbohydr Polym2015 130:405-419.
5. Gholami H, Fard FR, Saharkhiz MJ, Ghani A. Yield and physicochemical properties of inulin obtained from Iranian chicory roots under vermicompost and humic acid treatments. Crops Prod 2018;123:610-616.
6. El-Esawi MA, Elkelish A, Elansary HO, Ali HM, Elshika M, Witczak J, Ahmad M. Genetic transformation and hairy root induction enhance the antioxidant potential of Lactuca serriola L. Oxid Med Cell Longev 2017;5604746.
7. Banerjee S, Singh S, Rahman LU. Biotransformation studies using hairy root cultures-a review. Biotechnol Adv 2012;30:461-468.
8. Wahyuni DK, Vidiyanti F, Purnobasuki H, Ermayanti TM, Prajoga B, Utami ESW. Agrobacterium rhizogenes mediated hairy root induction in Justicia gendarussa Burm. f. J Appl Environ Biol Sci 2015;5:87-93.
9. Wu CH, Dewir YH, Hahn EJ, Paek KY. Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots ofEchinacea angustifolia. J Plant Biol 2006;49:193.
10. Cao D, Hou W, Song S, Sun H, Wu C, Gao Y, Han T. Assessment of conditions affecting Agrobacterium rhizogenes-mediated transformation of soybean. Plant Cell Tiss Org Cult 2009;96:45-52.
11. Cui XH, Murthy HN, Wu CH, Paek KY. Sucrose-induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell Tiss Org Cult 2010;103:7-14.
12. Valimehr S, Sanjarian F, Sohi HH, Sharafi A, Sabouni F. A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma. Physiol Mol Biol Plants 2014; 20:351-356.
13. Higa A, Mori Y, Kitamura Y. Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus. J Plant Physiol 2010;167:870-878.
14. Liu L, Yang D, Liang T, Zhang H, He Z, Liang Z. Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots. Plant Cell Rep 2016;35:1933-1942.
15. Yin S, Gao W, Liang Y, Wang J, Liu H, Wei C, Zuo B. Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla. Acta Physiol Plant 2013;35:1579-1585.
16. Moharrami F, Hosseini B, Sharafi A, Farjaminezhad M. Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture of Hyoscyamus reticulatus L. elicited by iron oxide nanoparticles. In Vitro Cell Dev Biol-Plant 2017;53:104-111.
17. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473-497.
18. Schenk RU, Hildebrandt AC. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 1972;50:199-204.
19. Jain VM, Karibasappa GN, Dodamani AS, Mali GV. Estimating the carbohydrate content of various forms of tobacco by phenol-sulfuric acid method. J Educ Health Promot 2017;4:90.
20. Kabirnataj S, Nematzadeh G, Zolal J, Talebi AF. High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb. Acta Agric Slov 2016;107:321-333.
21. Hanafy MS, Asker MS, Shabrawi HE, Matter MA. Agrobacterium rhizogenes-mediated genetic transformation in Cichorium spp.: hairy root production, inulin and total phenolic compounds analysis. J Hortic Sci Biotech 2018;93:605-613.
22. Zhang Z, Liao H, Lucas WJ. Molecular mechanisms underlying phosphate sensing, signaling, and adaptation in plants. J Integr Plant Biol 2014;56:192-220.
23. Liu L, Yang D, Xing B, Zhang H, Liang Z. Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses. Molecules 2018;23:1132.
24. Ward JT, Lahner B, Yakubova E, Salt DE, Raghothama KG. The effect of iron on the primary root elongation of Arabidopsis during phosphate deficiency. Plant Physiol 2008;147: 1181-1191.
25. Zargar SM, Agrawal GK, Rakwal R, Fukao Y. Quantitative proteomics reveals role of sugar in decreasing photosynthetic activity due to Fe deficiency. Front Plant Sci 2015;6:592.
26. López-Millán AF, Morales F, Andaluz S, Gogorcena Y, Abadia A, Rivas JDL, Abadia J. Responses of sugar beet roots to iron deficiency. Changes in carbon assimilation and oxygen use. Plant Physiol 2000;124:885-898. | ||
|
آمار تعداد مشاهده مقاله: 1,424 تعداد دریافت فایل اصل مقاله: 1,047 |
||
