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Development of a novel and specialized cultivation method for isolating Helicobacter pullorum from chicken meat | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 1 - شماره پیاپی 74، 2021، صفحه 76-80 اصل مقاله (268.11 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2020.38193.5560 | ||
| نویسندگان | ||
| H. Akhlaghi1؛ S. H. Emadi Chashmi2؛ A. Jebelli Javan* 3 | ||
| 1DVM Student, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 2Department of Clinical Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| چکیده | ||
| Background: It has become established that Helicobacter pullorum could be isolated from raw chicken meat. Aims: This study was aimed to develop a novel culture method (protocol B) to isolate H. pullorum from chicken meat by adding some modifications to the traditional culture method (protocol A), and as a consequence to compare their sensitivity, specificity, and the accuracy of these methods with polymerase chain reaction (PCR) test. Methods: 400 chicken meat samples were collected from various retail markets and supermarkets. Each sample was processed by protocol A, protocol B, and PCR test. Results: Out of 400 samples, 77 (19.25%), and 163 (40.75%) were culture-positive by protocol A and protocol B, respectively. Using PCR test as a gold standard, 196 (49%) samples were identified as H. pullorum. The specificity for both protocols was determined 100%, while the sensitivity of protocol B and protocol A was assessed 83% and 39%, respectively. Also, the higher and lower accuracy belonged to protocol B (92%) and protocol A (70%), respectively. Conclusion: The methodology designed herein can provide a suitable, approximately sensitive, specific, and accurate method to cultivate H. pullorum from chicken meat. | ||
| کلیدواژهها | ||
| Culture method؛ Helicobacter pullorum؛ PCR؛ Sensitivity؛ Specificity | ||
| مراجع | ||
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