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In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 1 - شماره پیاپی 74، 2021، صفحه 65-71 اصل مقاله (412.32 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2020.37714.5508 | ||
| نویسندگان | ||
| P. Xu* 1؛ H. Fotina1؛ T. Fotina1؛ S. Wang2 | ||
| 1Department of Veterinary Expertise, Microbiology, Zoohygiene and Safety and Quality of Animals’ Products, Faculty of Veterinary Medicine, Sumy National Agrarian University, 40021 Sumy, Ukraine | ||
| 2Department of Veterinary Expertise, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003 Xinxiang, China | ||
| چکیده | ||
| Background: Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. Aims: This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from the milk of Ukraine Holstein dairy cows in order to develop a general in vitro model. Methods: The milk from a three-year-old lactating dairy cow was used as a source of the epithelial cell, characteristics of BMECs were examined using real time cell assay (RTCA), immunocytochemistry (ICC), reverse transcription-polymerase chain reaction (RT-PCR), and Western blot (WB). Results: The results showed that BMECs can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells. Conclusion: The established BMECs retained MEC characteristics and secreted β-caseins even when grew on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands. | ||
| کلیدواژهها | ||
| Bovine mammary epithelial cell؛ β-casein؛ Cobblestone morphology؛ Mammary disease؛ Ukraine Holstein dairy cow | ||
| مراجع | ||
|
Ahn, JK; Aoki, N; Adachi, T; Mizuno, Y; Nakamura, R and Matsuda, T (1995). Isolation and culture of bovine mammary epithelial cells and establishment of gene transfection conditions in the cells. Biosci. Biotech. Bioch., 59: 59-64.
Akers, R (2006). Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. J. Dairy Sci., 89: 1222-1234.
Anand, V; Dogra, N; Singh, S; Kumar, SN; Jena, MK; Malakar, D; Dang, AK; Mishra, BP; Mukhopadhyay, TK; Kaushik, JK and Mohanty, AK (2012). Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line. PloS One. 7: e40469.
Aoki, N (2006). Regulation and functional relevance of milk fat globules and their components in the mammary gland. Biosci. Biotech. Bioch., 70: 2019-2027.
Bartek, J; Durban, E; Hallowes, R and Taylor-papadimitriou, J (1985). A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J. Cell Sci., 75: 17-33.
Buehring, GC (1990). Culture of mammary epithelial cells from bovine milk. J. Dairy Sci., 73: 956-963.
Capuco, AV and Choudhary, RK (2020). Determinants of milk production: Understanding population dynamics in the bovine mammary epithelium. J. Dairy Sci., 103: 2928-2940.
Ceriani, RL; Taylor-Papadimitriou, J; Peterson, JA and Brown, P (1979). Characterization of cells cultured from early lactation milks. In vitro Cell Dev. Biol. Anim., 15: 356-362.
Cui, LL; Bu, PD; Wang, JQ and Luo, SP (2006). In vitro culture of cow mammary epithelial cells. Xinjiang Agri. Sci., 43: 394-398.
Cui, XJ; Wang, T; Liu, BC; Tao, L and Wang, X (2013). Isolation and identification of mammary epithelial cells from milk. Biotech. Bull., 3: 107-113.
Danielson, KG; Oborn, CJ; Durban, EM; Butel, JS and Medina, D (1984). Epithelial mouse mammary cell line exhibiting normal morphogenesis in vivo and functional differentiation in vitro. Proc. Natl. Acad. Sci. USA. 81: 3756-3760.
Du, J; Di, HS and Wang, GL (2007). Establishment of a bovine epithelial mammary cell line and ultrastructural changes when exposed to heat stress. Chin. J. Biotechnol., 23: 471-476.
Duan, AQ; Pang, CY; Zhu, P; Deng, TX; Lu, XR; Ma, XY; Liang, SS and Liang, XW (2017). Isolation, culture and identification of buffalo mammary epithelial cells from milk. China Anim. Hus. Vet. Med., 44: 3243-3249.
Duchler, M; Schmoll, F; Pfneisl, F; Brem, G and Schellander, K (1998). OMEG II: a new ovine mammary epithelial cell line. Biol. Cell. 90: 199-205.
Ebner, KE; Hoover, CR; Hageman, EC and Larson, BL (1961). Cultivation and properties of bovine mammary cell cultures. Exp. Cell Res., 23: 373-385.
Franke, WW; Appelhans, B; Schmid, E; Freudenstein, C; Osborn, M and Weker, K (1979). Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation. 15: 7-25.
Fu, NY; Nolan, E; Lindeman, GJ and Visvader, JE (2020). Stem cells and differentiation hierarchy in mammary gland development. Physiol. Rev., 100: 489-523.
Gaffney, EV; Polanowski, FP; Blackburn, SE; Lambiase, JT and Burke, RE (1976). Cultures of normal human mammary cells. Cell Differ. 5: 69-81.
German, T and Barash, I (2002). Characterization of an epithelial cell line from bovine mammary gland. In vitro Cell Dev. Biol. Anim., 38: 282-292.
Gibson, CA; Vega, JR; Baumrucker, CR; Oakley, CS and Welsch, CW (1991). Establishment and characterization of bovine mammary epithelial cell lines. In vitro Cell Dev. Biol. Anim., 27A: 585-594.
Hu, H; Wang, JQ; Bu, DP; Wei, HY; Zhou, LY; Li, FD and Loor, JJ (2009). In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow. PLoS One. 4: e7636.
Ilan, N; Barash, I; Gootwine, E and Shani, M (1998). Establishment and initial characterization of the ovine mammary epithelial cell line NISH. In vitro Cell Dev. Biol. Anim., 34: 326-332.
Jedrzejczak, M and Szatkowska, I (2014). Bovine mammary epithelial cell cultures for the study of mammary gland functions. In vitro Cell Dev. Biol. Anim., 50: 389-398.
Joshi, PS; Modur, V; Cheng, J; Robinson, K and Rao, K (2017). Characterization of immortalized human mammary epithelial cell line HMEC 2.6. Tumor Biol., 2017: 1-12.
Kaushik, R; Singh, KP; Kumari, A; Singh, MK; Manik, RS; Palta, P; Singla, SK and Chauhan, MS (2013). Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line. In vitro Cell Dev. Biol. Anim., 49: 1-7.
Ke, MW; Hsu, JT; Jiang, YN; Cheng, WTK and Ju, YT (2012). Characterzation of hTERT-Immortalized caprine mammary epithelial cells. Reprod. Domest. Anim., 47: 553-561.
Kitchen, BJ (1981). Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. J. Dairy Res., 48: 167-188.
Kittrell, FS; Oborn, CJ and Medina, D (1992). Development of mammary preneoplasias in vivo from mouse mammary epithelial cell lines in vitro. Canner Res., 52: 1924-1932.
KuMuRA, H; Tanaka, A; Abo, Y; Yui, S; Shimazaki, K; Kobayashi, E and Sayama, K (2001). Primary culture of porcine mammary epithelial cells as a model system for evaluation of milk protein expression. Biosci. Biotech. Bioch., 65: 2098-2101.
Lorraine, M and Sordillo, MS (2018). Mammary gland immunobiology and resistance to mastitis. Vet. Clin. North Am. Food Anim. Pract., 34: 507-523.
McGrath, MF (1987). A novel system for mammary epithelial cell culture. J. Dairy Sci., 70: 1967-1980.
Ogorevc, J; Prpar, S and Dove, P (2009). In vitro mammary gland model: establishment and characterization of a caprine mammary epithelial cell line. Acta Agr. Slov., 94: 133-138.
Pantschenko, AG; Woodcock-Mitchell, J; Bushmich, SL and Yang, TJ (2000). Establishment and characterization of a caprine mammary epithelial cell line (CMEC). In vitro. 36: 26-37.
Pipe, SW; Miao, HZ; Butler, SP and Calcaterra, J (2011). Functional factor VIII made with von Willebrand factor at high levels in transgenic milk. J. Thromb. Haemostasis. 9: 2235-2242.
Puissant, C; Attal, J and Houdebine, L (1990). The hormonal control of ovine β-lactoglobulin gene in cultured ewe mammary explants. Reprod. Nutr. Dev., 30: 245-251.
Rose, MT; Aso, H; Yonekura, S; Komatsu, T; Hagino, A; Ozutsumi, K and Obara, Y (2002). In vitro differentiation of a cloned bovine mammary epithelial cell. J. Dairy Res., 69: 345-355.
Schmid, E; Franke, WW; Grund, C; Schiller, DL; Kolb, H and Paweletz, N (1983). An epithelial cell line with elongated myoid morphology derived from bovine mammary gland. Exp. Cell Res., 146: 309-328.
Schmid, E; Schiller, DL; Grund, C; Stadler, J and Franke, WW (1983). Tissue type-specific expression of intermediate filament proteins in a cultured epithelial cell line from bovine mammary gland. J. Cell Biol., 96: 37-50.
Shamay, A and Gertler, A (1986). A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells. Cell Biol. Int. Rep., 10: 923-929.
Strange, R; Li, F; Friis, RR; Reichmann, E; Haenni, B and Burri, PH (1991). Mammary epithelial differentiation in vitro: minimum requirements for a functional response to hormonal stimulation. Cell Growth Differ. 2: 549-559.
Su, F; Liu, X; Liu, GH; Yu, Y; Wang, YS; Jin, YP; Hu, GD; Song, H and Zhang, Y (2013). Establishment and evaluation of a stable cattle type II Alveolar epithelial cell line. PLoS One. 8: e76036. Sun, Y; Lin, C and Chou, Y (2006). Establishment and characterization of a spontaneously immortalized porcine mammary epithelial cell line. Cell Biol. Int., 30: 970-976.
Taylor-Papadimitriou, J; Shearer, M and Tilly, R (1992). Some properties of cells cultured from early-lactation human milk. J. Natl. Cancer I. 58: 1563-1571.
Taylor-Papadimitriou, J; Stampfer, M; Bartek, J; Lewis, A; Boshell, M; Lane, EB and Leigh, IM (1989). Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivophenotypes and influence of medium. J. Cell Sci., 94: 403-413.
Tong, HL; Li, QZ; Gao, XJ and Yin, DY (2012). Establishment and characterization of a lactating dairy goat mammary gland cell line. In vitro Cell Dev. Biol. Anim., 48: 149-155.
Xu, W; Chen, Q; Jia, Y; Deng, J; Jiang, S; Qin, Q; Wang, X; Yang, X and Jiang, H (2019). Isolation, characterzation, and SREBP1 functional analysis of mammary epithelial cell in buffalo. J. Food Biochem., 43: e12997.
Zhang, H; Liu, T; Li, B; Zhang, K; Wang, D; Liu, Y; Ge, L; Jiang, Y and Su, F (2020). Establishment of a stable β-casein protein-secreted Laoshan dairy goat mammary epithelial cell line. Front Vet. Sci., 13: 501.
Zhao, K; Liu, HY; Zhou, MM and Liu, JX (2010). Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis. Cell Biol. Int., 34: 717-721.
Zheng, YM; He, XY and Zhang, Y (2010). Characteristics and EGFP expression of goat mammary gland epithelial cells. Reprod. Domest. Anim., 45: e323-331.
Zhong, K; Wang, YL and Yang, GY (2006). Research progress of somatic cells in breast secretions. China Anim. Hus. Vet. Med., 12: 39-42. | ||
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