| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,685 |
| تعداد دریافت فایل اصل مقاله | 11,580,422 |
The effect of docetaxel on survival, fertilization rate and apoptosis-related genes mRNA expression in mouse metaphase II oocytes following vitrification | ||
| Iranian Journal of Veterinary Research | ||
| دوره 22، شماره 1 - شماره پیاپی 74، 2021، صفحه 40-47 اصل مقاله (594.94 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2020.38128.5554 | ||
| نویسندگان | ||
| Z. Khodabandeh1؛ I. Jamhiri1؛ N. Dehghani2؛ H. Daneshpazhouh3؛ B. Namavar Jahromi4؛ M. Dianatpour5؛ S. Alaee* 6 | ||
| 1Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Department of Biology, College of Science, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran | ||
| 3Ph.D. Student in Biology, Department of Biology, Payam Noor University, Tehran, Iran | ||
| 4Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 6Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Docetaxel is beneficial in oocyte cryopreservation. Aims: The effect of docetaxel, on the survival, fertilization rate and mRNA expression of apoptosis-related genes of vitrified mature oocytes was investigated. Methods: Mature oocytes were divided into eight experimental groups, including I) control, II) docetaxel, III) docetaxel + cryoprotectant agent 1 (CPA1), IV) docetaxel + CPA2, V) docetaxel + vitrification 1 (Vit1), VI) docetaxel + Vit2, VII) Vit1, and VIII) Vit2. The survival and fertilization rates, and the mRNA expression level of Bcl-xl, Bax and caspase-3 as apoptosis-related genes were evaluated. Results: The survival rates in Vit1, and Vit2 groups were significantly lower than in the control group (P<0.05). The fertilization rates in docetaxel + Vit1, docetaxel + Vit2, Vit1, and Vit2 were significantly lower than the control, docetaxel, and related groups using docetaxel and CPAs. Bax expression was significantly increased in groups which oocytes vitrified. Also, its expression in the Vit2 group increased significantly in comparison to the docetaxel + Vit2 group. The expression of the Bcl-xl gene was downregulated in docetaxel + CPA2, docetaxel + Vit2 and Vit2 compared to docetaxel group. The Bax/Bcl-xl ratio significantly increased in docetaxel + CPA2, docetaxel + Vit1, docetaxel + Vit2, Vit1 and Vit2 groups compared to control, docetaxel and the docetaxel + CPA1 group. Caspase-3 expression significantly increased in all six groups in comparison to the control, and docetaxel groups. Its expression significantly increased in the Vit1 and Vit2 groups in comparison with docetaxel + Vit1, and docetaxel + Vit2, respectively. Conclusion: Docetaxel ameliorates the damages to oocytes during vitrification by altering the expression of apoptosis-related genes and its effects are dependent on the vitrification solution used in cryopreservation of oocytes. | ||
| کلیدواژهها | ||
| Apoptosis؛ Docetaxel؛ Fertilization؛ Oocytes؛ Vitrification | ||
| مراجع | ||
|
Abdollahi, M; Salehnia, M; Salehpour, S and Ghorbanmehr, N (2013). Human ovarian tissue vitrification/warming has minor effect on the expression of apoptosis-related genes. Iran. Biomed. J., 17: 179-186. Almasi, TS; Rouzbehi, A; Aliabadi, E; Haeri, A; Sadeghi, Y and Hosseini, A (2009). Developmental consequences of mouse cryotop-vitrified oocyte and embryo using low concentrated cryoprotectants. Int. J. Reprod. Biomed., 7: 181-188. Amidi, F; Khodabandeh, Z and Mogahi, MHN (2018). Comparison of the effects of vitrification on gene expression of mature mouse oocytes using cryotop and open pulled straw. Int. J. Fertil. Steril., 12: 61-67. Anchamparuthy, V; Pearson, R and Gwazdauskas, F (2010). Expression pattern of apoptotic genes in vitrified thawed bovine oocytes. Reprod. Domest. Anim., 45: e83-e90. Bakhtari, A; Nazari, S; Alaee, S; Kargar-Abarghouei, E; Mesbah, F; Mirzaei, E and Molaei, MJ (2020). Effects of dextran-coated superparamagnetic iron oxide nanoparticles on mouse embryo development, antioxidant enzymes and apoptosis genes expression, and ultrastructure of sperm, oocytes and granulosa cells. Int. J. Fertil. Steril., 14: 161-170. Bogdał, MN; Kochańczyk, M and Lipniacki, T (2013). Levels of pro-apoptotic regulator Bad and anti-apoptotic regulator Bcl-xL determine the type of the apoptotic logic gate. BMC Syst. Biol., 7: 67. Chasombat, J; Nagai, T; Parnpai, R and Vongpralub, T (2015). Pretreatment of in vitro matured bovine oocytes with docetaxel before vitrification: Effects on cytoskeleton integrity and developmental ability after warming. Cryobiology. 71: 216-223. Chian, RC; Wang, Y and Li, YR (2014). Oocyte vitrification: advances, progress and future goals. Int. J. Assist. Reprod. Genet., 31: 411-420.
Council, NR (2011). Guide for the care and use of laboratory animals. 8th Edn., Washington D. C., USA, National Academies Press. PP: 1-219.
Cristofani, R; Marelli, MM; Cicardi, ME; Fontana, F; Marzagalli, M; Limonta, P; Poletti, A and Moretti, RM (2018). Dual role of autophagy on docetaxel-sensitivity in prostate cancer cells. Cell Death Dis., 9: 1-9.
De Bem, TH; Adona, PR; Bressan, FF; Mesquita, LG; Chiaratti, MR; Meirelles, FV and Leal, Cl (2014). The influence of morphology, follicle size and Bcl-2 and bax transcripts on the developmental competence of bovine Oocytes. Reprod. Domest. Anim., 49: 576-583. Dehghani, N; Dianatpour, M; Hosseini, SE; Khodabandeh, Z and Daneshpazhouh, H (2019). Overexpression of mitochondrial genes (mitochondrial transcription factor A and cytochrome c oxidase subunit 1) in mouse metaphase II oocytes following vitrification via cryotop. Int. J. Mol. Sci., 44: 406-414. Dhali, A; Anchamparuthy, VM; Butler, SP; Pearson, RE; Mullarky, IK and Gwazdauskas, FC (2009). Effect of droplet Dhali vitrification on development competence, actin cytoskeletal integrity and gene expression in in vitro cultured mouse embryos. Theriogenology. 71: 1408-1416. Domanska, UM; Timmer-Bosscha, H; Nagengast, WB; Munnink, THO; Kruizinga, RC; Ananias, HJ; Kliphuis, NM; Huls, G; De Vries, EG and de Jong, IJ (2012). CXCR4 inhibition with AMD3100 sensitizes prostate cancer to docetaxel chemotherapy. Neoplasiam. 14: 709-718. Ebrahimi, B; Valojerdi, MR; Eftekhari-Yazdi, P and Baharvand, H (2010). In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes. J. Assist. Reprod. Genet., 27: 239-246. Esmaeilzadeh, F; Hamedi, L; Mehrabani, D; Bidshahri, A and Tanideh, N (2015). The effect of vitrification on follicular morphology of ovarian rat. J. Fasa Univ. Med. Sci., 5: 185-195. Fu, XW; Shi, WQ; Zhang, QJ; Zhao, XM; Yan, CL; Hou, YP; Zhou, GB; Fan, ZQ; Suo, L and Wusiman, A (2009). Positive effects of Taxol pretreatment on morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrification of in vitro matured porcine oocytes. Anim. Reprod. Sci., 115: 158-168. Gharenaz, NM; Movahedin, M and Mazaheri, Z (2018). Effects of re-vitrification of mouse morula and early blastocyst stages on apoptotic gene expression and developmental potential. Cell J., 19: 614-619.
Ghorbani, M; Sadrkhanlou, R; Nejati, V; Ahmadi, A and Tizroo, G (2012). The effects of dimethyl sulfoxide and ethylene glycol as vitrification protectants on different cleavage stages of mouse embryo quality. Vet. Res. Forum., 3: 245-249. Hou, L; Zhang, Y; Baoli, Y; Yanzhou, Y; Bo, L and Wu, J (2018). Oocyte-G1 promotes male germ cell apoptosis through activation of Caspase-3. Gene. 670: 22-30. Hwang, IS and Hochi, S (2014). Recent progress in cryopreservation of bovine oocytes. BioMed. Res. Int., 2014: 570647.
Ilani, M; Alaee, S; Khodabandeh, Z; Jamhiri, I and Owjfard, M (2018). Effect of titanium dioxide nanoparticles on the expression of apoptotic markers in mouse blastocysts. Toxicol. Environ. Chem., 100: 228-234. Khodabandeh Jahromi, Z; Amidi, F; Habibi, A; Ebrahimi, M; Nori Mugehe, SMH; Nouri, MS; Sobhani, A; Mehranian, K; Abbasi, M and Habibi Roudkenar, M (2010). Expression of heat shock protein (HSP A1A) and MnSOD genes following vitrification of mouse MII oocytes with cryotop method. Cell J., 12: 113-119. Kuwayama, M (2007). Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 67: 73-80. Leon, P; Campos, V; Kaefer, C; Begnini, K; McBride, A; Dellagostin, O; Seixas, F; Deschamps, J and Collares, T (2013). Expression of apoptotic genes in immature and in vitro matured equine oocytes and cumulus cells. Zygote. 21: 279-285. Luciano, AM; Modina, S; Vassena, R; Milanesi, E; Lauria, A and Gandolfi, F (2004). Cyclic role of intracellular adenosine 3´, 5´-monophosphate concentration and oocyte-cumulus cells communications on the acquisition of the developmental competence during in vitro maturation of bovine oocyte. Biol. Reprod., 70: 465-472. Moawad, AR; Choi, I; Zhu, J and Campbell, KH (2011). Ovine oocytes vitrified at germinal vesicle stage as cytoplast recipients for somatic cell nuclear transfer (SCNT). Cell Reprogram., 13: 289-296.
Morató, R; Izquierdo, D; Paramio, MT and Mogas, T (2008). Cryotops versus open-pulled straws (OPS) as carriers for the cryopreservation of bovine oocytes: effects on spindle and chromosome configuration and embryo development. Cryobiology. 57: 137-141. Parsanezhad, ME; Namavar Jahromi, B; Rezaee, S; Kooshesh, L and Alaee, S (2017). The effect of four different gonadotropin protocols on oocyte and embryo quality and pregnancy outcomes in IVF/ICSI cycles; a randomized controlled trial. Iran. J. Med. Sci., 42: 57-65. Pitchayapipatkul, J; Somfai, T; Matoba, S; Parnpai, R; Nagai, T; Geshi, M and Vongpralub, T (2017). Microtubule stabilisers docetaxel and paclitaxel reduce spindle damage and maintain the developmental competence of in vitro-mature bovine oocytes during vitrification. Reprod. Fert. Develop., 29: 2028-2039. Rahbar, A and Abbasi, M (2020). A brief clinical overview of etiological factors in infertility. J. Infertil. Reprod. Biol., 8: 6-8. Razi, MH; Razi, Y; Sabeti, P; Ghasemi-Esmailabad, S and Pourmasumi, S (2014). Correlation between women age and oocyte quality, embryo formation and pregnancy outcomes in assisted reproductive technology (ART) cycles: a retrospective analysis. J. Infertil. Reprod. Biol., 2: 108-114. Roozbehi, A (2013). Mouse oocytes and embryos cryotop-vitrification using low concentrated solutions: Effects on Shahedi, A; Hosseini, A; Khalili, MA and Yeganeh, F (2017). Vitrification affects nuclear maturation and gene expression of immature human oocytes. Res. Mol. Med., 5: 27-33. Shamas-Din, A; Kale, J; Leber, B and Andrews, DW (2013). Mechanisms of action of Bcl-2 family proteins. Cold. Spring Harb. Perspect. Biol., 5: a008714.
Shams Mofarahe, Z; Ghaffari Novin, M and Salehnia, M (2020). Folliculogenesis-associated genes expression in human vitrified ovarian tissue after xenotransplantation in γ irradiated mice. Cell J., 22: 350-357. Shams Mofarahe, Z; Salehnia, M; Ghaffari Novin, G; Ghorbanmehr, N and Gholami Fesharaki, M (2017). Expression of folliculogenesis-related genes in vitrified human ovarian tissue after two weeks in vitro culture. Cell J., 19: 18-26. Sharifi, S; Barar, J; Hejazi, MS and Samadi, N (2015). Doxorubicin changes Bax/Bcl-xL ratio, caspase-8 and 9 in breast cancer cells. Adv. Pharm. Bull., 5: 351-359. Sharma, Y and Sharma, M (2020). Sperm cryopreservation: principles and biology. J. Infertil. Reprod. Biol., 8: 43-48. Somfai, T; Ozawa, M; Noguchi, J; Kaneko, H; Karja, NW; Farhudin, M; Dinnyés, A; Nagai, T and Kikuchi, K (2007). Developmental competence of in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: effect of cryopreservation on oocyte antioxidative system and cell cycle stage. Cryobiology. 55: 115-126.
Xiao, H; Verdier-Pinard, P; Fernandez-Fuentes, N; Burd, B; Angeletti, R; Fiser, A; Horwitz, SB and Orr, GA (2006). Insights into the mechanism of microtubule stabilization by Taxol. Proc. Natl. Acad. Sci., 103: 10166-10173. Xu, B; Strom, J and Chen, QM (2011). Dexamethasone induces transcriptional activation of Bcl-xL gene and inhibits cardiac injury by myocardial ischemia. Eur. J. Pharmacol., 668: 194-200. Yang, MY and Rajamahendran, R (2002). Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro. Anim. Reprod. Sci., 70: 159-169. Ye, J; Coulouris, G; Zaretskaya, I; Cutcutache, I; Rozen, S and Madden, TL (2012). Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 13: 134. | ||
|
آمار تعداد مشاهده مقاله: 697 تعداد دریافت فایل اصل مقاله: 762 |
||