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Dual effect of Sapogenins extracted from Spirulina platensis on telomerase activity in two different cell lines | ||
| Molecular Biology Research Communications | ||
| دوره 10، شماره 1، 2021، صفحه 1-4 اصل مقاله (198.68 K) | ||
| نوع مقاله: Short communication | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2020.38230.1537 | ||
| نویسندگان | ||
| Mahboobeh Akbarizare؛ Hamideh Ofoghi* ؛ Mahnaz Hadizadeh | ||
| Iranian Research Organization for Science and Technology (IROST), Tehran, Iran | ||
| چکیده | ||
| Spirulina platensis is a photosynthetic filamentous, edible cyanobacterium that is known as a superfood. In this study, sapogenins were extracted from the spirulina and the effects of these compounds on telomerase activity were evaluated in MCF7 and HDF cell lines using Telomeric Repeat Amplification Protocol and ELIZA assay. The highest increase in telomerase activity was observed at 0.004 mg/ml of sapogenin by 26% ±20.5 in MCF7 cells, while in HDF cells in the same concentration telomerase activity decreased down to 47%±0.48 and the highest inhibition of telomerase activity was observed at 0.070 mg/ml of sapogenins from Spirulina by 68%±0.43. In conclusion, a compound could play a role as a telomerase activator in one cell line while it could play another role as a telomerase inhibitor in another cell line so introducing compounds as a telomerase inhibitor (anticancer) or as a telomerase activator (anti-aging) should be done with discreet. | ||
| کلیدواژهها | ||
| Spirulina platensis؛ Sapogenins؛ Telomerase activator؛ Telomerase inhibitor | ||
| مراجع | ||
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1. Mahdieh G, Fazilati M, Izadi M, Pilehvarian A, Nazem H. Investigation of ACE inhibitory effect and antioxidant activity of peptide extracted from Spirulina platensis. Chem Methodol J 2020;4:172-180. 2. Mathur M. Bioactive Molecules of Spirulina: A Food Supplement. In: Mérillon JM., Ramawat K. (eds) Bioactive Molecules in Food. 2018;1-22. Springer, Cham. https://doi.org/10.1007/978-3-319-54528-8_97-1 3. Turner KJ, Vasu V, Griffin DK. Telomere biology and human phenotype. Cells 2019;8:73. 4. Wai LK. Telomeres, telomerase, and tumorigenesis, a review. Med Gen Med 2004;6:19. 5. Shay J, Wright W. Telomeres and telomerase: three decades of progress. Nat Rev Genet 2019;20:299-309. 6. Shanab S, Mostafa S, Shalaby E, Mahmoud G. Aqueous extracts of microalgae exhibit antioxidant and anticancer activities. Asian Pac J Trop Biomed 2012;2:608-615. 7. Majinda RT. Extraction and isolation of Saponins. Natural products isolation. Methods Mol Biol 2012;864:415-426. 8. Kuljanabhagavad T, Thongphasuk P, Chamulitrat W, Winkd M. Triterpene saponins from Chenopodium quinoa Willd. Phytochemistry 2008;69:1919-1926. 9. Fayyad RJ, Ali AN, Dwaish AS, Abboodi AK. Anti cancer activity of Spirulina platensis methanolic extracts against L20B and MCF7 human cancer cell lines. Plant Arch 2019;19: 1419-1426. 10.Yurtcu E, Iseri OD, Sahin FI. Effects of silymarin and silymarin-doxorubicin applications on telomerase activity of human hepatocellular carcinoma cell line HepG2. J BUON 2015;20: 555-561. 11. Parzonko A, Naruszewicz M. Silymarin inhibits endothelial progenitor cells' senescence and protects against the antiproliferative activity of rapamycin: preliminary study. J Cardiovasc Pharmacol 2010;56:610-618. 12. Harley CB, Liu W, Blasco M, Vera E, Andrews WH, Briggs LA, Raffaele JM. A natural product telomerase activator as part of a health maintenance program. Rejuvenation Res 2011;14:45-56. 13. Ilbeigi D, Nourbakhsh M, Khaghani S, Einollahi N, Kheiripour N, Gholinejad Z, Alaee M, Saberian M. Enterolactone reduces telomerase activity and the level of its catalytic subunit in breast cancer cells. cell J 2017;19:37-43. 14. Wojtyla A, Gladych M, Rub B. Human telomerase activity regulation. Mol Biol Rep 2011;38:3339-3349. 15. Chang JT, Lu YC, Chen YJ, Tseng CP, Chen YL, Fang CW, Cheng AJ. hTERT phosphorylation by PKC is essential for telomerase holoprotein integrity and enzyme activity in head neck cancer cells. Br J Cancer 2006;94:870-878. | ||
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