| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,795 |
| تعداد دریافت فایل اصل مقاله | 11,580,476 |
Nucleotide mutation analyses of isolated lentogenic newcastle disease virus in live bird market | ||
| Molecular Biology Research Communications | ||
| دوره 9، شماره 4، 2020، صفحه 181-188 اصل مقاله (252.75 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2020.38061.1530 | ||
| نویسندگان | ||
| Jola Rahmahani1؛ Aisyah Nikmatuz Zahro2؛ Indah Laili Rahmawati3؛ Nurvita Putih3؛ Innah Wulandari3؛ Fedik Abdul- Rantam* 1، 4 | ||
| 1Laboratory of Virology and Immunology, Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Indonesia, 60115 | ||
| 2Bachelor of Veterinary Science, Faculty of Veterinary Medicine, Airlangga University, Indonesia, 60115 | ||
| 3Master of Vaccinology, Faculty of Veterinary Medicine, Airlangga University, Indonesia, 60115 | ||
| 4Stem Cell Research and Development Center, Airlangga University, Indonesia, 60115 | ||
| چکیده | ||
| Newcastle Disease (ND) is a major viral disease in Indonesia. It is an RNA virus belongs to Paramyxovirinae. It is well known that RNA virus is easily to mutate. In some cases, this mutation could generate virulence alteration. It is noted that mutation of NDV which has avirulent amino acid sequence on the cleavage site, could mutate to be virulent Newcastle Disease Virus (NDV). It is needed to analyze the nucleotide and amino acid mutations and the effect of those to its virulence. The aim of this study was to analyze nucleotide and amino acid mutations of original isolated Lentogenic Newcastle Disease Virus (NDV). Samples were collected from cloacal swab of native chicken (Gallus gallus domesticus) suspected to be infected by Lentogenic NDV from live bird markets. They were inoculated into embryonated eggs, to isolate the virus. HA and HI assays were conducted to confirm that they were NDV. Positive samples were processed into serial passages in embryonated egg to observe their death time. Samples caused mortality of the embryonated eggs more than 90 hours post infection were suspected as Lentogenic NDV. They were processed to RT-PCR then sequenced. Lentogenic NDV confirmation was done by comparing amino acid at Fusion protein cleavage site of the samples to Lasota/JF950510. Nucleotide and amino acid mutations were analyzed. The result showed that some nucleotide mutations were capable to change sequences of amino acid but the virulence of the samples remained the same to the reference sequence. | ||
| کلیدواژهها | ||
| NDV؛ RNA virus؛ mutations؛ virulence | ||
| مراجع | ||
|
1. Fenner. 2011. Fenner’s Veterinary Virology 4thedition: Paramyxoviridae. Elsevier. 2. Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR, Roisman B. Fields Virology 2013; LIPPINCOT WILLIAMS & WILKINS. 3. OIE. 2012. OIE Manual of Diagnostic Test and Vaccines for Terrestrial Animals in Newcastle Diseases (Infection with Newcastle Diseases Virus). 4. Alamares JG. Newcastle disease virus virulence: Mechanism of the interferon antagonistic activity of the V protein and characterization of a putative virulence-specific antibody to the attachment protein. 2008. Any disertation. Medical School. University of Massachusetts. 5. Seal BS, King DJ, Sellers HS. The avian response to Newcastle diseases Virus. Dev Comp Immunol 2000;24:257-268. 6. Ganar K, Das M, Sinha S, Kumar S. Newcastle diseases virus: Current status and our understanding. Virus Res 2014;184:71-81. 7. Errington W, Emmerson PT. Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein. J Gen Virol 1997; 78:2335-2339. 8. Moran IS, Castano SC, Barroso IM, Kostetsky EY, Zhadan G, Gomez J, Shnyrov VL, Villar E. Thermal stability of matrix protein from Newcastle diseases virus. Inter J Biol Macromol 2013;61:390-395. 9. Samal S. Role of fusion protein in Newcastle disease virus pathogenesis [Disertation]. Faculty of Graduated School. University of Maryland 2012. 10. Miller PJ, Haddas R, Simanov L, Lublin A, Rehmani SF, Wajid A, Bibi T, Khan TA, Yaqub T, Setiyaningsih S, Afonso CL. Identification of new sub-genotypes of virulent Newcastle disease virus with potential panzootic features. Infect Genet Evol 2015;29:216-229. 11. Horvarth CM, Lamb RA. Studies on the fusion peptide of a Paramyxovirus fusion glycoprotein : Roles of conserved residues in cell fusion. J Virol 1992;66:2443-2445. 12. Yuan P, Swanson KW, Leser GP, Paterson RG, Lamb RA, Jardetzky TS. Structure of the Newcastle disease virus Hemagglutinin-Neuraminidase (HN) ectodomain reveals a four-helix bundle stalk. Proc Natl Acad Sci USA 2011;108:14920-14925. 13. Porotto M, Salah ZW, Gui L, DeVito I, Jurgens EM, Lu H, Yokoyama CC, Palermo LM, Lee KK, Moscona A. Regulation of Paramyxovirus fusion activation: the hemagglutinin-neuraminidase protein stabilizes the fusion protein in a pretriggered state. J Virol 2012;86: 12838-12848. 14. Krishnamurthy S, Samal SK. Nucleotide sequences of the trailer, Nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence. J Gen Virol 1998;79:2419-2424. 15. Jenkins GM, Rambaut A, Pybus OG, Holmes EC. Rates of molecular evolution in RNA viruses : A quantitative phylogenetic analysis. J Mol Evol 2002;54:156-165. 16. Miller PJ, Kim LM, Ip HS, Afonso CL. Evolutionary dynamics of Newcastle disease virus. Virology 2009;391:64-72. 17. Alexander DJ. Newcastle disease, other avian Paramyxoviruses and pneumovirus infection. Rev Sci Tech 2000;19:443-462. 18. Alexander DJ, Bell JG, Alders RG. A Technology Review : Newcastle disease with special emphasis on its effect on village chickens. In : FAO Animal Production and Health. 2004. 19. Aldous EW, Alexander DJ. Detection and differentiation of Newcastle disease virus (avian Paramyxovirus type 1). Avian Pathol 2001;30:117-128. 20. Dharmayanti NI, Hartawan R, Hewajuli DA, Indriani R. Phylogenic analysis of genotype VII of Newcastle diseases virus in Indonesia. Afri J Microbiol Res 2014;8:1368-1374. 21. Chukwudi OE, Chukwuemeka ED, Mary U. Newcastle disease virus shedding among healthy commercial chickens and its epidemiological importance. Pak Vet J 2012;32:354-356. 22. Meng C, Qiu X, Yu S, Li C, Sun Y, Chen Z, Liu K, Zhang X, Tan L, Song C, Liu G, Ding C. Evolution of Newcastle disease virus quasispecies diversity and enhanced virulence after passage through chicken air sacs. J Virol 2016;90:2052-2063. 23. Lauring AS, Andino R. Quasispecies theory and the behaviour of RNA virus. PLoS Pathog 2010;6:e1001005. 24. Yan Y, Samal SK. Role of intergenic sequences in Newcastle disease virus RNA transcription and pathogenesis. J Virol 2008;82:1323-1331. 25. Ayala AJ, Dimitrov KM, Becker CR, Goraichuk IV, Arns CW, Bolotin VI, Ferreira HL, Gerilovych AP, Goujgoulova GV, Martini MC, Muzyka DV, Orsi MA, Scagion GP, Silva RK, Solodiankin OS, Stegniy BT, Miller PJ, Afonso CL. Presence of vaccine-derived Newcastle disease viruses in wild birds. PLoS One 2016;11:e0162484. 26. Sa X, Paldural A, Nayak B, Samuel A, Bharoto EE, Prajitno TY, Collins PL, Samal SK. Complete genome sequence of Newcastle disease virus strains circulating in chicken populations of Indonesia. J Virol 2012:5969-5970. | ||
|
آمار تعداد مشاهده مقاله: 1,099 تعداد دریافت فایل اصل مقاله: 754 |
||