| تعداد نشریات | 24 |
| تعداد شمارهها | 862 |
| تعداد مقالات | 7,672 |
| تعداد مشاهده مقاله | 13,668,449 |
| تعداد دریافت فایل اصل مقاله | 11,743,017 |
Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay | ||
| Molecular Biology Research Communications | ||
| دوره 9، شماره 3، 2020، صفحه 117-121 اصل مقاله (114.01 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2020.37381.1506 | ||
| نویسندگان | ||
| Fargol Abdali1؛ Saeid Hosseinzadeh2؛ Enayat Berizi* 1؛ Maryam Pourmontaseri2 | ||
| 1Nutrition Research Center, Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens)in unpasteurized dairy products consumed in Shiraz province. In this study conducted in 2016, 238 unpasteurized dairy products including 48 raw milk, 48 yogurt, 46 cheeses, 48 dough and 48 ice cream samples, were purchased from the retail market in Shiraz province and were examined by a specific PCR assay. This study showed positive 5/04% out of 238 unpasteurized dairy products including 9 out of 48 (18/75%) raw milk samples and 3 out of 48 (6.25%) yogurt samples). Contamination was not detected in samples of dough, cheese and traditional ice cream. The results also showed that among 12 positive samples, 6 samples were contaminated with B. abortus (including 4 milk samples and 2 yogurt samples), 2 samples were contaminated with B. melitensis (including 2 Milk samples) and 4 samples were contaminated simultaneously with B. abortus and B. melitensis (including 3 milk samples and 1 yogurt sample). The present study suggests the unpasteurized dairy products as the major sources of brucellosis in Shiraz province, South of Iran; thus, to prevent brucellosis in human, the consumption of pasteurized milk and dairy products is highly recommended. | ||
| کلیدواژهها | ||
| Consumption؛ Brucella؛ Dairy products؛ PCR | ||
| مراجع | ||
|
1.Young EJ, Corbel MJ. Brucellosis: clinical and laboratory aspects. CRC press 1989. 2. Gwida M, Al Dahouk S, Melzer F, Rösler U, Neubauer H, Tomaso H. Brucellosis–regionally emerging zoonotic disease? Croat Med J 2010;51:289-295. 3. Masoudian M, Derakhshandeh A, Ghahramani Seno MM. Brucella melitensis and Mycobacterium tuberculosis depict overlapping gene expression patterns induced in infected THP-1 macrophages. Iran J Vet Res 2015;16:368-373. 4. Young EJ. An overview of human brucellosis. Clin Infec Dis 1995;21:283-289. 5. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. The New Eng J Med 2005; 352:2325-2336. 6. Esmaeili H. Brucellosis in Islamic republic of Iran. J Med Bacteriol 2014;3:47-57. 7. Jawetz E, Melnick JL, Adelberg EA. Review of medical microbiology. The Pediat Inf Dis J 1983;2:176. 8. Corbel MJ. Brucellosis in humans and animals: World Health Organization; 2006. 9. Spanu V, Spanu C, Virdis S, Cossu F, Scarano C, De Santis EPL. Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep's milk cheese. Int J Food Microbiol 2012;153:53-57. 10. Izadi A, Moslemi E, Tabatabaei Panah A, Kheiri Manjili H. Brucella spp. detection in dairy products using nested and hemi nested PCR techniques. Ann Biol Res 2014;5:124-131. 11. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet Microbiol 2002;90:435-446. 12. Gupta VK, Verma DK, Rout PK, Singh SV, Vihan VS. Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rum Res 2006;65:79-84. 13. Al Nakkas AF, Wright SG, Mustafa AS, Wilson S. Single-tube, nested PCR for the diagnosis of human brucellosis in Kuwait. Ann Trop Med Parasitol 2002;96:397-403. 14. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994;32:2660-2666. 15. Doganay M, Aygen B. Human brucellosis: an overview. Int J Infect Dis 2003;7:173-82. 16. Shakerian A. Study of contamination rate in craw milk and its traditional products with Brucella abortus, and Brucella mellitensis in Isfahan and Chaharmahal and Bakhtiari Provinces. 2012. J Shahrekord Univ Med Sci 2015;17. 17. Bryan FL. Epidemiology of milk-borne diseases. J Food Prot 1983;46:637-649. 18. Miyashiro S, Scarcelli E, Piatti RM, Campos FR, Vialta A, Keid LB, Dias RA, Genoves ME. Detection of Brucella abortus DNA in illegal cheese from São Paulo and Minas Gerais and differentiation of B19 vaccinal strain by means of the polymerase chain reaction (PCR). Braz J Microbiol 2007;38:17-22. 19. Talei BAAAB. Isolation, identification and biotyping of Brucella spp. from milk product at Basrah Province. Basrah J Vet Res 2010;9:152-162. 20. Arasoglu T, Gulluce M, Özkan H, Adiguzel A, Şahin F. PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey. Turk J Med Sci 2013;43:501-518. 21. Shafei B, Ahmadi M, Dastmalchi SH. Diagnosis of Brucella abortus and Brucella melitensis in the milk of cattle and sheep in Kordestan province by polymerase chain reaction. 2013. 22. Esmaeili H, Ekhtiyar ZH, Ebrahimzadeh H, Partovi R, Marhamati KB, Hamedi M, Khaji L. Evaluation of the national sheep and goat brucellosis control program in Iran. Arak Uni Med Sci 2012;14:9-20. | ||
|
آمار تعداد مشاهده مقاله: 1,149 تعداد دریافت فایل اصل مقاله: 848 |
||