| تعداد نشریات | 24 |
| تعداد شمارهها | 916 |
| تعداد مقالات | 8,202 |
| تعداد مشاهده مقاله | 15,918,337 |
| تعداد دریافت فایل اصل مقاله | 13,466,899 |
Production of transgenic Paulownia tomentosa (Thunb.) steud. using chitosan nanoparticles to express antimicrobial genes resistant to bacterial infection | ||
| Molecular Biology Research Communications | ||
| مقاله 3، دوره 9، شماره 2، 2020، صفحه 55-62 اصل مقاله (671.27 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2019.35331.1454 | ||
| نویسنده | ||
| Eman Tawfik Hussien* | ||
| Lecturer of Genetics and Genetic Engineering, Faculty of Science, Helwan University, Egypt | ||
| چکیده | ||
| Paulownia tomentosa (Thunb.) Steud. is a very important hard woody plant, an extremely fast-growing tree and produce timber. Therefore, there is a demand to produce transgenic Paulownia plant resistant to bacterial infection. Microbial infection (especially bacterial one) is serious sever and cause a loss in plant productivity as they bear upon the character and amount of plant product. Two phytopathogenic bacteria were chosen to consider their effect on Paulownia tomentosa. These two bacterial species were Erwinia carotovora and Pseudomonas aeruginosa. Two thionin genes (AT1G12660 and AT1G12663) were selected. They produce antimicrobial peptides to resist this bacterial infection. Chitosan nanoparticle is a novel technology in genetic transformation into plant tissues. Chitosan nanoparticles were used in a ratio of 1:1 with the plasmid DNA carrying thionin genes independently. Characterization for chitosan nanoparticles was applied to determine the conditions of genetic transformation. The new transgenic P. tomentosa lines produced are partially resistant to these two bacterial infections compared to non-transgenic lines. The inhibitory percentage in the transgenic lines ranged from 8 to 21% wherein the non-transgenic the inhibitory percentage of P. tomentosa leaves ranged from 53-24%. Likewise, it is noticed that is Paulownia tomentosa less infectious than Erwinia carotovora. In conclusion, I recommend using chitosan nanoparticle is an excellent way for gene transformation into plant tissues. Also, manipulate the idea of using thionin as antimicrobial genes to resist bacterial infection for different plant species. | ||
| کلیدواژهها | ||
| Chitosan nanoparticle؛ Erwinia carotovora؛ thionin genes transformation | ||
| مراجع | ||
|
1. Litwińczuk W, Bochnia E. Development of royal paulownia (Paulownia tomentosa steud) in vitro shoot cultures under the influence of different saccharides. Acta Sci Pol Hortorum Cultus 2012;11:3-13
2. Stewart WM, Vaidya BN, Mahapatra AK, Terrill TH, Joshee N. Potential use of multipurpose Paulownia elongata tree as an animal feed resource. Am J Plant Sci 2018;9:1212-1227.
3. Schneiderova´ K, Smejkal K. Phytochemical profile of Paulownia tomentosa (Thunb). Steud. Phytochem Rev 2015;14:799-833.
4. Shtereva L, Vassilevska-Ivanova R, Karceva T, Kraptchev B. Micropropagation of six Paulownia genotypes through tissue culture. J Cent Eur Agric 2014;15:147-156.
5. Al-Sagheer AA, Abd El-Hack ME, Alagawany M, Naiel MA, Mahgoub SA, Badr MM, Hussein EOS, Alowaimer AA, Swelum AA. Paulownia leaves as a new feed resource: Chemical composition and effects on growth, carcasses, digestibility, blood biochemistry, and intestinal bacterial populations of growing rabbits. Animals 2019;9:95.
6. Prajapat R, Marwal A, Jha PN. Erwinia carotovora associated with potato: A critical appraisal with respect to Indian perspective. Int J Curr Microbiol App Sci 2013;2:83-89.
7. Yu L, Qin X Y, Du J, Wang AY, Zhao YY, Shen DJ, Sun YX, Huang Q. Bacterial leaf spot of tobacco caused by Pseudomonas aeruginosa in China. Plant Pathology 2008;57:774.
8. Kenneth T. Opportunistic infections caused by Pseudomonas aeruginosa. In: Todar’s online Textbook of Bacteriology, 2008 http://textbookofbacteriology.net/kt toc.html.
9. Hoshikawa K, Ishihara G, Takahashi H, Nakamura I. Enhanced resistance to gray mold (Botrytis cinerea) in transgenic potato plants expressing thionin genes isolated from Brassicaceae species. Plant Biotechnol 2012;29:87-93.
10. Özgümüs S, Gök MK, Pabuccuoglu S. Chitosan: Gene Delivery. Encyclopedia of Biomedical Polymers and Polymeric Biomaterials 2014;1-15.
11. Lu Y, Chanroj S, Zulkifli L Johnson MA, Uozumi N, Cheung A, Sze H. Pollen tubes lacking a pair of K+ transporters fail to target ovules in Arabidopsis. Plant Cell 2011;23:81-93.
12. Czechowska-Biskup R, Jarosińska D, Rokita B, Ulański P, Rosiak JM. Determination of degree of deacetylation of chitosan-comparison of methods. PCACD 2012;17:5-20.
13. Zetasizer Nano Series User Manual. MAN 0317, Issue 1.1, 2004 (Malvern Instruments Ltd.)
14. Silva VJ. Preparation and characterization of chitosan nanoparticles for gene delivery. Tecnico Lisboa 2013;1-10.
15. Mansouri S, Cuie Y, Winnik F, Shi Q, Lavigne P, Benderdour M, Beaumont E, Fernands JC. Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials 2006;27:2060-2065.
16. Kiang T, Wen J, Lim HW, Leong KW. The effect of the degree of chitosan deacetylation on the efficiency of gene transfection. Biomaterials 2004;25:5293-5301.
17. Abdel-Razik AB, Hammad IA, Tawfik E. Transformation of thionin genes using chitosan nanoparticle into potato plant to be resistant to fungal infection. IOSR-JBB 2017; 3:1-13.
18.Tawfik E. Production of transgenic potato expressing antimicrobial thionin gene. Lambert academic publishing. OmniScriptum Publishing Group, Germany. 2018.
19. Rogers SO, Bendich AJ. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 1985;5:69-76.
20. Kumar TS, kumar SS, Preetha D. Screening of Pathogenic Strains of Pseudomonas aeruginosa from Clinical samples using Lactuca sativa as a plant model. Int J Curr Microbiol App Sci 2015;4:566-577.
21. Hallaj-Nezhadi S, Valizadeha H, Dastmalchia S, Baradaranc B, Jalalia MB, Dobakhtid F, Lotfipoure F. Preparation of chitosan-plasmid DNA nanoparticles encoding interleukin-12 and their expression in CT-26 colon carcinoma cells. J Pharm Pharm Sci 2011;14:181-195.
22. Ing LY, Zin NM, Sarwar A, Katas H. Antifungal activity of chitosan nanoparticles and correlation with their physical properties. Int J Biomater 2012;1:9.
23. Stec B. Plant thionins- -the structural perspective. Cell Mol Life Sci 2006;63:1370 -1385.
24. Plattner S, Gruber C, Stadlmann J, Widmann S, Gruber CW, Altmann F, Bohlmann H. Isolation and characterization of a thionin proproteinprocessing enzyme from barley. J Biol Chem 2015;290:18056-18067.
25. Tam JP, Wang S, Wong KH, Tan WL. Antimicrobial peptides from plants. Pharmaceuticals 2015;8:711-757.
26. Petranka Z, Maya Y, Kaloyan K, Ivanka P. Antimicrobial peptides with plant origin. IJNFS 2016;5:5-9. | ||
|
آمار تعداد مشاهده مقاله: 2,083 تعداد دریافت فایل اصل مقاله: 1,343 |
||