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Molecular characterization and DNA methylation profile of Libyodrilus violaceous from oil polluted soil | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 9، شماره 2، 2020، صفحه 45-53 اصل مقاله (368.64 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2019.35242.1449 | ||
| نویسندگان | ||
| Aemere Ogunlaja* 1، 2؛ Vikas Sharma2، 3؛ Meenu Ghai4؛ Johnson Lin2 | ||
| 1Department of Biological Sciences, Redeemer’s University, Ede Osun state, Nigeria | ||
| 2School of Life Sciences, Discipline of Microbiology, University of Kwazulu-Natal, Durban, South Africa | ||
| 3Department of Biotechnology Engineering, Ambala college of Engineering and Applied Research, Devsthali, P.O Sambhalkha, Ambala-133101, Haryana, India | ||
| 4School of Life Sciences, Discipline of Genetics, University of Kwazulu-Natal, Durban, South Africa | ||
| چکیده | ||
| Studies on earthworms using molecular markers are rare in Africa except a handful from South Africa. Reports on Libyodrilus violaceous,an earthworm found in West Africaare available including their metal tolerance and bioaccumulation capacity but their molecular characterization and ecotoxicology studies are scarce. In this study, triplicate L. violaceous specimens were collected from four locations within a petroleum polluted site and one in a control site, ≃1Km away from point of spill. DNA was extracted and 18S rRNA and 16S rRNA genes were amplified and sequenced. DNA methylation of their 18S rRNA gene was determined using Methylation specific PCR (MSP) method. Phylogenetic trees generated for 18S rRNA and 16S rRNA genes grouped L. violaceous within the Eudrilidae family concurrent with its conventional grouping and MSP results indicate no methylation in L. violaceous population from this site. | ||
| کلیدواژهها | ||
| Oil spill؛ Pollution؛ Natural attenuation؛ DNA Barcode؛ Phylogeny | ||
| مراجع | ||
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