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Molecular detection of Mycobacterium leprae using RLEP-PCR in post elimination era of leprosy | ||
| Molecular Biology Research Communications | ||
| مقاله 3، دوره 9، شماره 1، 2020، صفحه 17-22 اصل مقاله (249.19 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2020.35658.1464 | ||
| نویسندگان | ||
| Partha Sarathi Mohanty* 1؛ Farhah Naaz1؛ Avi Kumar Bansal1؛ Dilip kumar1؛ Sandeep Sharma1؛ Mamta Arora2؛ Haribhan Singh1؛ Pradhumn Katara1؛ Namrata Soni1؛ Shripad A. Patil1؛ Manjula Singh3 | ||
| 1Department of Epidemiology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India | ||
| 2Clinical Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India | ||
| 3Epidemiology and Communicable Diseases Division, Indian Council of Medical Research, V Ramalingaswami Marg, Ansari Nagar, New Delhi, India | ||
| چکیده | ||
| Leprosy is considered as a contagious disease and is still a health problem in several countries including India. Diagnosis of leprosy is based either on clinical findings or on acid fast bacilli staining. Due to low sensitivity of acid fast bacilli staining most of the leprosy cases were remained undetected. The present study aims toassess the efficacy of RLEP-PCR in the field condition where majority of the patients are acid fast bacilli negative and have early disease. A total of 80 suspected leprosy cases were recruited. Slit skin smear samples were taken for microscopy and molecular experimentation. DNA was extracted and RLEP-PCR was executed for all the 80 samples. To establish the statistical correlation χ2 test and Fisher's exact test were made. To elucidate the sensitivity of the test Receiver Operating Characteristic (ROC) was drawn. These 80 leprosy patients comprised of 38 paucibacillary and 42 multibacillary leprosy cases. Of 80 leprosy patients 18 (22.5%) were AFB positive while 53 (66.25%) leprosy cases were RLEP-PCR positive. The results of test of significance (P=0.0001) and Cohen's kappa coefficient (κ) (0.614) indicated that the RLEP-PCR is a better diagnostic tool over AFB microscopy in case detection of leprosy. From the findings we concluded that RLEP-PCR could be used for the definitive detection of leprosy cases in accordance with the clinical findings in the field condition in the post elimination era of leprosy. | ||
| کلیدواژهها | ||
| Leprosy؛ RLEP-PCR؛ Paucibaccilary؛ Multibacillary | ||
| مراجع | ||
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1. World Health Organization. “Leprosy Fact Sheet”. Updated on January 2018. Assessed on 19.07.2019. http://www.who.int/mediacentre/factsheets/fs101/en/ 2. Mayboroda OA, van Hooij A, Derks R, van den Eeden SJ, Dijkman K, Khadge S, Thapa P, Kunwar CB, Hagge DA, Geluk A. Exploratory urinary metabolomics of type 1 leprosy reactions. Int J Infect Dis 2016;45:46-52. 3. Fine PE, Steme JA, Pönnighaus JM, Bliss L, Saui J, Chihana A, Munthali M, Wamdorff DK. Household and dwelling contact as risk factors for leprosy in northern Malawi. Am J Epidemiol 1997;146:91-102. 4. Moet FJ, Pahan D, Schuring RP, Oskam L, Richardus JH. Physical distance, genetic relationship, age, and leprosy classification are independent risk factors for leprosy in contacts of patients with leprosy. J Infect Dis 2006;193:346-353. 5. Yan W, Xing Y, Yuan LC, de Yang R, Tan FY, Zhang Y, Li HY. Application of RLEP real-time PCR for detection of M. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy. Am J Trop Med Hyg 2014;90:524-529. 6. Martinez AN, Talhari C, Moraes MO, Talhari S. PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic. PLoS Negl Trop Dis 2014;8:e2655. 7. Phetsuksiri B, Rudeeaneksin J, Supapkul P, Wachapong S, Mahotarn K, Brennan PJ. A simplified reverse transcriptase PCR for rapid detection of Mycobacterium leprae in skin specimens. FEMS Immunol Med Microbiol 2006;48:319-328. 8. Torres P, Camarena JJ, Gomez JR, Nogueira JM, Gimeno V, Navarro JC, Olmos A. Comparison of PCR-mediated amplification of DNA and the classical methods for detection of Mycobacterium leprae in different types of clinical samples in leprosy patients and contacts. Lepr Rev 2003;74:18-30. 9. Santos AR, Balassiano V, Oliveira ML, Pereira MA, Santos PB, Degrave WM, Suffys PN. Detection of Mycobacterium leprae DNA by polymerase chain reaction in the blood of individuals, eight years after completion of anti-leprosy therapy. Mem Inst Oswaldo Cruz 2001;96:1123-1133. 10. Martinez AN, Britto CF, Nery JA, Sampaio EP, Jardim MR, Sarno EN, Moraes MO. Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy. J Clin Microbiol 2006;44:3154-3159. 11. Donoghue HD, Holton J, Spigelman M. PCR primers that can detect low levels of Mycobacterium leprae DNA. J Med Microbiol 2001;50:177-182. 12. Banerjee M, Capozzoli M, McSweeney L, Sinha D. Beyondkappa: A review of interrater agreement measures. Canadian J Stat 1999;27:3-23. 13. Mohanty PS, Naaz F, Katara D, Misba L, Kumar D, Dwivedi DK, Tiwari AK, Chauhan DS, Bansal AK, Tripathy SP, Katoch K. Viability of Mycobacterium leprae in the environment and its role in leprosy dissemination. Indian J Dermatol Venereol Leprol 2016;82:23-27. 14. Truman RW, Andrews PK, Robbins NY, Adams LB, Krahenbuhl JL, Gillis TP. Enumeration of Mycobacterium leprae using real-time PCR. PLoS Negl Trop Dis 2008; 2:e328. 15. Shepard CC, McRae DH. A method for counting acid-fast bacteria. Int J Lepr Other Mycrobact Dis 1968;36:78-82. 16. Almeida EC, Martinez AN, Maniero VC, Sales AM, Duppre NC, Sarno EN, Santos AR, Moraes MO. Detection of Mycobacterium leprae DNA by polymerase chain reaction in the blood and nasal secretion of Brazilian household contacts. Mem Inst Oswaldo Cruz 2004;9 9:509-511. 17. Williams DL, Gillis TP, Booth RJ, Looker D, Watson JD. The use of a specific DNA probe and polymerase chain reaction for the detection of Mycobacterium leprae. J Infect Dis 1990; 162:193-200. 18. Woods SA, Cole ST. A rapid method for the detection of potentially viable Mycobacterium leprae in human biopsies: a novel application of PCR. FEMS Microbiol Lett 1989;53:305-309. 19. Goulart IM, Cardoso AM, Santos MS, Gonçalves MA, Pereira JE, Goulart LR. Detection of Mycobacterium leprae DNA in skin lesions of leprosy patients by PCR may be affected by amplicon size. Arch Dermatol Res 2007;299:267-271. 20. Reis EM, Araujo S, Lobato J, Neves AF, Costa AV, Gonçalves MA, Goulart LR, Goulart IM. Mycobacterium leprae DNA in peripheral blood may indicate a bacilli migration route and high-risk for leprosy onset. Clin Microbiol Infect 2014;20:447-452. 21. Siwakot S, Rai K, Bhattarai NR, Agarwal S, Khanal B. Evaluation of polymerase chain reaction (PCR) with slit skin smear examination (SSS) to confirm clinical diagnosis of Leprosy in eastern Nepal. PLoS Negl Trop Dis 2016,10:e0005220. | ||
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