| تعداد نشریات | 24 |
| تعداد شمارهها | 859 |
| تعداد مقالات | 7,646 |
| تعداد مشاهده مقاله | 13,578,486 |
| تعداد دریافت فایل اصل مقاله | 11,684,905 |
Genetic polymorphisms of Y-chromosome short tandem repeats (Y-STRs) in a male population from Golestan province, Iran | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 9، شماره 1، 2020، صفحه 11-16 اصل مقاله (234.87 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2020.35547.1462 | ||
| نویسندگان | ||
| Minoo Sayyari1؛ Ali Salehzadeh* 1؛ Mohammad Amin Tabatabaiefar2؛ Ali Abbasi3 | ||
| 1Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 2Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Iranian Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran | ||
| چکیده | ||
| Short Tandem Repeats (STRs), which are located out of pseudo-autosomal parts of the human Y chromosome and passed-down from fathers to the male offspring in a non-recombinant form, are regarded as appropriate markers for forensic purposes and evolutionary investigations. Few studies concerned the genotyping of Y chromosome short tandem repeats (Y-STRs) among the ethnic groups of the north of Iran, especially the province of Golestan which is a multiethnic region of Iran. Thus, in this work we investigated the frequency of Y-STR haplotypes among the male population from Golestan province, to elucidate their identity and kinship patterns. A total number of 106 unrelated male individuals participated in this study. Genomic DNA was extracted from blood samples and the multiplex polymerase chain reaction was employed to amplify DNA fragments. Genotyping was performed using capillary electrophoresis and, finally, allele polymorphisms, haplotype diversity (HD) and haplotype discrimination capacity (DC) were determined using GenAlEXv6.5 and Arlequin v5.3.2 software and compared to other regions of Iran. A total number of 87 unique haplotypes were determined. The highest and least allelic polymorphism was observed for the DYS385b and DYS391 loci, respectively. HD and DC were 0.9962 and 0.8207, respectively. In the case of locus with the least allelic variation, we didn’t observe any difference between the Gilan and Golestan but there was a difference between the Golestan and Mazandaran provinces. Our results indicated the efficiency of Y-STRs to be used as genetic markers for forensic medicine, and also the evolutionary comparison of different ethnic groups of Golestan, Iran. Also, a low genetic distance between the population of Golestan with other northern provinces was noticed. | ||
| کلیدواژهها | ||
| Allele؛ Haplotype diversity؛ Haplotype discrimination capacity | ||
| مراجع | ||
|
1. Butler JM. Advanced Topics in Forensic DNA Typing Methodology. Elsevier Inc 2012; 704p. 2. Butler JM. Fundamentals of Forensic DNA Typing: Lineage Markers: Y Chromosome. Elsevier Inc 2010;520p. 3. Butler JM. Recent Developments in Y Short tandem repeat and Y-single nucleotide polymorphism analysis. Forensic Sci Rev 2003;15:91-111. 4. Jobling MA. In the name of the father: surnames and genetics. Trends Genet 2001;17:353- 357. 5. Roewer L, Arnemann J, Spurr NK, Grzeschik KH, Epplen JT. Simple repeat sequences on the human Y-chromosome are equally polymorphic as their autosomal counterparts. Hum Genet 1992;89:389-394. 6. Jeffreys AJ. Highly variable minisatellites and DNA fingerprints. Biochem Soc Trans 1987; 15:309-317. 7. Nei M. Molecular evolutionary genetics. New York: Columbia university press 1987;512p. 8. Peakall R, Smouse PE. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 2012;28:2537-2539. 9. Excoffier L, Lischer HE. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 2010;10:564-567. 10. Sayyari M, Salehzadeh A, Tabatabai MA, Abbasi A. Profiling of 17 Y-STR loci in Mazandaran and Gilan provinces of Iran. Turk J Med Sci 2019;49:1277-1286. 11.Vatandoost N, Salehi AR, Kazemi M, Khosravi S, Eslami G, Kamali S. Genetic polymorphism of 8 Y-STR loci in native population of Isfahan province in central part of Iran. Ann Hum Biol 2017;44:175-179. 12. Tabrizi AA, Hedjazi A, Kerachian MA, Honarvar Z, Dadgarmoghaddam M, Raoofian R. Genetic profile of 17 Y-chromosome STR haplotypes in East of Iran. Forensic Sci Int Genet 2014;14:6-7. | ||
|
آمار تعداد مشاهده مقاله: 1,139 تعداد دریافت فایل اصل مقاله: 938 |
||