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Molecular identification of aviadenoviruses in broiler chickens suspected to inclusion body hepatitis in Golestan province, Iran | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 5، دوره 21، شماره 3 - شماره پیاپی 72، 2020، صفحه 198-202 اصل مقاله (314.57 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2020.34619.5113 | ||
| نویسندگان | ||
| S. Arazi1؛ M. Mayahi* 2؛ F. Talazadeh2؛ Z. Boroomand2؛ R. A. Jafari2 | ||
| 1Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Background: Fowl adenoviruses (FAdVs) are distributed widely throughout the world, and domestic avian species of all ages are susceptible. Fowl aviadenoviruses (FAdVs) can be separated into 5 different species (A-E) with various genotypes and 12 serotypes. Some geno- or serotypes induce hepatitis-hydropericardium syndrome (HPS), inclusion body hepatitis (IBH), and adenoviral gizzard erosion (AGE). Aims: Detect FAdVs serologically and molecularly and sequencing of FAdVs in broiler flocks in Golestan province. Methods: From December 2017 to June 2018 liver tissues and blood samples were collected from 31 broiler flocks suspected of IBH. Polymerase chain reaction (PCR) was applied on liver samples and the positive samples were sequenced and antibody against FAdVs was measured by enzyme-linked immunosorbent assay (ELISA). Results: Out of 31 flocks, the titers of 29 flocks (93.5%) were high in ELISA test for FAdVs and 22 flocks (70.96%) were positive in PCR test. Sequence analysis indicated the isolates belonged to D and E genotype of adenovirus. Conclusion: Inclusion body hepatitis caused by FAdVs, are spreading increasingly in broiler flocks of Golestan province and more attention and surveillance programs of breeder and broiler farms are needed to develop preventive measures. Moreover, vaccination of poultry farms in Iran should be considered by more complement studies. | ||
| کلیدواژهها | ||
| Adenovirus؛ Inclusion body hepatitis؛ Iran؛ Molecular identification؛ Serology | ||
| مراجع | ||
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Alvarado, IR; Villegas, P; EL-Attrache, J; Jensen, E; Rosales, G; Perozo, F and PurvisA, LB (2007). Genetic characterization, pathogenicity, and protection studies with an avian adenovirus isolate associated with inclusion body hepatitis. Avian Dis., 51: 27-32.
Balamurugan, V and Kataria, JM (2004). The hydro-pericardium syndrome in poultry - a current scenario. Vet. Res. Commun., 28: 127-148.
Cowen, BS (1988). Chicken embryo propagation of type I avian adenoviruses. Avian Dis., 32: 347-352.
De Herdt, P; Timmerman, T; Defoort, P; Lycke, K and Jaspers, R (2013). Fowl adenovirus infections in Belgian broilers: a ten-year survey. Vlaams Diergeneeskundig Tijdschrift. 82: 125-132.
Fadly, AM; Winterfield, RWF and Olander, HJ (1976). Role of the bursa of Fabricius in the pathogenicity of inclusion body hepatitis and infectious bursal disease viruses. Avian Dis., 20: 467-477.
Gomis, S; Goodhope, AR; Ojkic, AD and Willson, P (2006). Inclusion body hepatitis as a primary disease in broilers in Saskatchewan, Canada. Avian Dis., 50: 550-555.
Grafl, B; Aigner, F; Liebhart, D; Marek, A; Prokofieva, I; Bachmeier, J and Hess, M (2012). Vertical transmission and clinical signs in broiler breeders and broilers experiencing adenoviral gizzard erosion. Avian Path., 41: 599-604.
Hafez, MH (2011). Avian adenoviruses infections with special attention to inclusion body hepatitis/hydropericardium syndrome and egg drop syndrome. Pak. Vet. J., 31: 85-92.
Hess, M (2000). Detection and differentiation of avian adenoviruses: a review. Avian Path., 29: 195-206.
Hosseini, H and Morshed, R (2012). Molecular identification of fowl adenovirus associated with inclusion body hepatitis in Iran. Iran. J. Virol., 6: 7-12.
Marek, A; Günes, A; Schulz, E and Hess, M (2010). Classification of fowl adenoviruses by use of phylogenetic analysis and high-resolution melting-curve analysis of the hexon L1 gene region. J. Virol. Methods. 170: 147-154.
McFerran, JB and Adair, BM (1977). Avian adenoviruses. A review. Avian Path., 6: 189-217.
Mettifogo, E; Nuñez, LFN; Santander Parra, SH; Astolfi-Ferreira, CS and Piantino Ferreira, AJ (2014). Fowl adenovirus Group I as a causal agent of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS) outbreak in brazilian broiler flocks. Pesq. Vet. Bras., 34: 733-737.
Morshed, R; Hosseini, H; Ghalyanchi Langeroudi, A; Bozorgmehri Fard, MH and Charkhkar, S (2017). Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Dis., 61: 205-210.
Nateghi, E; Razmyar, J and Bassami, MR (2014). Molecular characterization of avian adenoviruses in Iranian broiler flocks. Iran. J. Vet. Res., 15: 164-167.
Ojkic, D; Martin, E; Swinton, J; Vaillancourt, JP; Boulianne, M and Gomis, S (2008). Genotyping of Canadian isolates of fowl adenoviruses. Avian Path., 37: 95-100.
Ono, M; Okuda, Y; Yazawa, S; Shibata, I; Sato, S and Okada, K (2003). Outbreaks of adenoviral gizzard erosion in slaughtered broiler chickens in Japan. Vet. Rec., 153: 775-779.
Steer, PA; O’Roureke, D; Ghorashi, SA and Noormohammadi, AH (2011). Application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. Aust. Vet. J., 89: 184-192.
Tabib Ghafari, P; Borooand, Z; Rezaie, A; Mayahi, M and Eftekharian, S (2017). Detection and identification of avian adenovirus in broiler chickens suspected of inclusion body hepatitis in Khuzestan, Iran during 2015-2016. Iran. J. Vet. Sci. Tech., 19: 41-45. Tafti, AK; Asasi, K and Namazi, F (2016). Clinicopathological characteristics of acute inclusion body hepatitis ourbreak in broiler chickens in Iran. Bul. J. Vet. Med., 19: 163-168.
Zadravec, M; Slavec, B; Krapež, U; Kaján, GL; Račnik, J; Juntes, P; Cizerl, RJ; Benkó, M and Rojs, OZ (2011). Inclusion body hepatitis associated with fowl adenovirus type 8b in broiler flock in Slovenia - a case report. Slov. Vet. Res., 48: 107-113. | ||
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