| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,740 |
| تعداد دریافت فایل اصل مقاله | 11,580,452 |
Effect of prostaglandins E2 and F2α on granulosa cell apoptosis in goat ovarian follicles | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 4، دوره 21، شماره 2 - شماره پیاپی 71، 2020، صفحه 97-102 اصل مقاله (337.57 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2020.35165.5175 | ||
| نویسندگان | ||
| A. K. Sharma1؛ R. K. Sharma* 2 | ||
| 1Ph.D. Student in Reproductive Physiology, Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra-136119, India | ||
| 2Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra-136119, India | ||
| چکیده | ||
| Background: The goat is a multi-purpose animal producing meat, milk, hide, fiber and manure, and hence plays a significant role in providing supplementary income and livelihood to the huge number of asset-poor ranchers and landless workers of rural India. Aims: The present study is aimed to investigate the in vitro effects of prostaglandins E2 (PGE2) and F2α (PGF2α) on ovarian granulosa cells of a goat. Methods: The healthy, slightly atretic and atretic antral follicles of goats with diameter ranging from 3-8 mm were cultured with PGE2 and PGF2α (1.0 μg/ml) along with control for 24 h. Histomorphological analysis was done in order to study the prostaglandins induced changes in granulosa cells of all the three categories of follicles. The acridine orange (AO) and methylene blue (MB) staining were used to study the apoptotic index in all the three categories of follicles and the collected data were analyzed by ANOVA with Duncan post Hoc test. Results: Prostaglandins E2 revealed positive while PGF2α showed a negative effect on the rescue of apoptosis in granulosa cells. The PGE2 treated follicles revealed a reduction in the attributes of apoptosis in granulosa cells while PGF2α showed an increased in the apoptotic characteristics. Conclusion: The present study depicted that granulosa cell viability in vitro is dependent on the continuous supply of survival and growth factors and prostaglandin of E series synthesis is a crucial step in the suppression of granulosa cell apoptosis while that of F series induces apoptosis. | ||
| کلیدواژهها | ||
| Apoptosis؛ Granulosa cells؛ Prostaglandins؛ Ovaries | ||
| مراجع | ||
|
Ainsworth, L; Baker, RD and Armstrong, DT (1975). Pre-ovulatory changes in follicular fluid prostaglandin F levels in swine. Prostaglandins. 9: 915-925.
Ainsworth, L; Tsang, BK; Downey, BR; Baker, RD; Marcus, GJ and Armstrong, DT (1979). Effects of indomethacin on ovulation and luteal function in gilts. Bio. Reprodn., 21: 401-411.
Armstrong, DT (1981). Prostaglandins and follicular functions. Reproduction. 62: 283-291.
Armstrong, DT and Grinwich, DL (1972). Blockade of spontaneous and LH-induced ovulation in rats by indomethacin, an inhibitor of prostaglandin biosynthesis. Prostaglandins. 1: 21-28.
Armstrong, DT; Moon, YS and Zamecnik, J (1974). Evidence for a role of ovarian prostaglandins in ovulation. In: Mougdal, NR (Ed.), Gonadotropins and gonadal function. (1st Edn.), Elsevier Inc., PP: 343-356.
Bauminger, S and Lindner, HR (1975). Periovulatory changes in ovarian prostaglandin formation and their hormonal control in the rat. Prostaglandins. 9: 737-751.
Bowolaksono, A; Nishimura, R; Hojo, T; Sakumoto, R; Acosta, TJ and Okuda, K (2008). Anti-apoptotic roles of prostaglandin E2 and F2alpha in bovine luteal steroidogenic cells. Bio. Reprodn., 79: 310-317.
Broaddus, VC; Yang, L; Scavo, LM; Ernst, JD and Boylan, AM (1996). Asbestos induces apoptosis of human and rabbit pleural mesothelial cells via reactive oxygen species. J. Clinic. Investig., 98: 2050-2059.
Dave, U; Shousha, S and Westaby, D (2001). Methylene blue staining: is it really useful in Barrett’s esophagus? Gastro. Endosco., 53: 333-335.
Goff, AK and Armstrong, DT (1983). Changes in responsiveness of rat granulosa cells to prostaglandin E2 and follicle-stimulating hormone during culture. Cana. J. Physio. Pharmacol., 61: 608-613.
Goff, AK; Zamecnik, J; Ali, M and Armstrong, DT (1978). Prostaglandin I2 stimulation of granulosa cell cyclic AMP production. Prostaglandins. 15: 875-879.
Grinwich, DL; Kennedy, TG and Armstrong, DT (1972). Dissociation of ovulatory and steroidogenic actions of luteinizing hormone in rabbits with indomethacin, an inhibitor of prostaglandin biosynthesis. Prostaglandins. 1: 89-96.
Hillier, SG; Zeleznik, AJ and Ross, GT (1978). Independence of steroidogenic capacity and luteinizing hormone receptor induction in developing granulosa cells. Endocrinology. 102: 937-946.
Lau, IF; Saksena, SK and Chang, MC (1974). Prostaglandins F and ovulation in mice. Reproduction. 40: 467-469.
Li, J and Tsang, BK (1995). Prostaglandins mediate the stimulation of deoxyribonucleic acid synthesis by transforming growth factor α in hen granulosa cells during ovarian follicular development. Bio. Reprodn., 52: 1050-1058.
Maia, JrH; Barbosa, I and Coutinho, EM (1978). Inhibition of ovulation in marmoset monkeys by indomethacin. Fert. Ster., 29: 565-570.
Manchanda, R; Kim, JM and Tsang, BK (2001). Role of prostaglandins in the suppression of apoptosis in hen granulosa cells by transforming growth factor alpha. Reproduction. 122: 91-101.
Nafisi, S; Saboury, AA; Keramat, N; Neault, JF and Tajmir-Riahi, HA (2007). Stability and structural features of DNA intercalation with ethidium bromide, acridine orange and methylene blue. J. Mol. Struct., 827: 35-43.
O’Grady, JP; Caldwell, BV; Auletta, FJ and Speroff, L (1972). The effects of an inhibitor of prostaglandin synthesis (indomethacin) on ovulation, pregnancy, and pseudopregnancy in the rabbit. Prostaglandins. 1: 97-106.
Orczyk, GP and Behrman, HR (1972). Ovulation blockade by aspirin or indomethacin-in vivo evidence for a role of prostaglandin in gonadotrophin secretion. Prostaglandins. 1: 3-20.
Pearse, AGE (1968). Histochemistry, theoretical and applied: theoretical and applied. 3rd Edn., Boston, USA.
Sharma, RK and Bhardwaj, JK (2009). Ultrastructural characterization of apoptotic granulosa cells in caprine ovary. J. Micro., 236: 236-242.
Stacey, NE and Pandey, S (1975). Effects of indomethacin and prostaglandins on ovulation of goldfish. Prostaglandins. 9: 597-607.
Triebwasser, WF; Clark, MR; LeMaire, WJ and Marsh, JM (1978). Localization and in vitro synthesis of prostaglandins in components of rabbit preovulatory Graafian follicles. Prostaglandins. 16: 621-632.
Tsang, BK; Ainsworth, L; Downey, BR and Armstrong, DT (1979). Pre-ovulatory changes in cyclic AMP and prostaglandin concentrations in follicular fluid of gilts. Prostaglandins. 17: 141-148.
Wallach, EE; de la Cruz, A; Hunt, J; Wright, KH and Stevens, VC (1975). The effect of indomethacin on HMG-HCG induced ovulation in the rhesus monkey. Prostaglandins. 9: 645-658.
Yadav, VK; Lakshmi, G and Medhamurthy, R (2005). Prostaglandin F2α-mediated activation of apoptotic signaling cascades in the corpus luteum during apoptosis involvement of caspase-activated DNase. J. Bio. Chem., 280: 10357-10367.
Yang, NS; Marsh, JM and LeMaire, WJ (1974). Post ovulatory changes in the concentration of prostaglandins in rabbit Graafian follicles. Prostaglandins. 6: 37-44. | ||
|
آمار تعداد مشاهده مقاله: 663 تعداد دریافت فایل اصل مقاله: 451 |
||