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Ostrich (Struthio camelus) primordial germ cells in embryonic blood and presumptive gonad: characterization by PAS and immunohistochemistry | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 9، دوره 20، شماره 4 - شماره پیاپی 69، 2019، صفحه 299-303 اصل مقاله (777.3 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2019.5509 | ||
| نویسندگان | ||
| B. Hassanzadeh1؛ A. Nabipour* 2؛ H. Dehghani3 | ||
| 1Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 3Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Background: Among the birds, chicken was the model used in the majority of studies on germ cells and other birds like quail, turkey and pheasant had some interest but ostrich has been lacking from these researches. Aims: The present study was conducted to confirm the presence of ostrich primordial germ cells (PGCs) in the embryonic blood and to determine the appropriate time for having them in the blood. Methods: Embryos were extracted in embryonic day (E) 6-12, their blood was obtained and the rest of the embryos were processed for histological examinations. Staining by periodic acid-Schiff (PAS) method and immunohistochemistry (IHC) using stage-specific embryonic antigen 1 (SSEA1) and stage-specific embryonic antigen 4 (SSEA4) antibodies (Abs) were used for identification of the PGCs. Results: While the blood circulating ostrich PGCs were SSEA1-negative and SSEA4-positive, in the presumptive gonads PGCs showed negative immunoreactivity for both Abs. Although the ostrich PGCs were PAS-positive in both blood and presumptive gonad, their PAS-positive contents reduced during development from E 10 to E 12. Conclusion: E 9, when the hind limb buds appear, is the best time for detecting PGCs in the ostrich embryonic blood. | ||
| کلیدواژهها | ||
| Immunohistochemistry؛ Ostrich embryo؛ PGC؛ Presumptive gonad؛ SSEA | ||
| مراجع | ||
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Ando, Y and Fujimoto, T (1983). Ultrastructural evidence that chick primordial germ cells leave the blood-vascular system prior to migrating to the gonadal anlagen. Dev. Growth Differ., 23: 345-352.
Armengol, C; Carretero, A; Nacher, V; Ruberte, J and Navarro, M (2007). Carbohydrate characterization of quail primordial germ cells during migration and gonadal differentiation. J. Anat., 210: 98-111.
Chan, AM (2010). Formation of presumptive embryoid bodies in undifferentiated gonadal cell cultures from the embryonic chick due to the presence of primordial germ cells. Ph.D. Thesis, Cornell University, Ithaca, US.
D’costa, S and Petitte, JN (1999). Characterization of stage-specific embryonic antigen-1 (SSEA-1) expression during early development of the turkey embryo. Int. J. Dev. Biol., 43: 349-356.
Encinas, G; Zogbi, C and Stumpp, T (2012). Detection of four germ cell markers in rats during testis morphogenesis: differences and similarities with mice. Cells Tissues Organs. 195: 443-455.
Ginsburg, M and Eyal-Giladi, H (1986). Temporal and spatial aspects of the gradual migration of primordial germ cells from the epiblast into the germinal crescent in the avian embryo. J. Embryol. Exp. Morphol., 95: 53-71.
Hamburger, V and Hamilton, HL (1951). A series of normal stages in the development of the chick embryo. J. Morph., 88: 45-92.
Han, JY (2009). Germ cells and transgenesis in chickens. Comp. Immunol. Microb., 32: 61-80.
Karagenç, L; Cinnamon, Y; Ginsburg, M and Petitte, JN (1996). Origin of primordial germ cells in the prestreak chick embryo. Dev. Genet., 19: 290-301.
Kim, JN; Lee, YM; Park, TS; Jung, JG; Cho, BW; Lim, JM and Han, JY (2005). Detection and characterization of primordial germ cells in pheasant (Phasianus colchicus) embryos. Theriogenology. 63: 1038-1049.
Kuwana, T; Maeda-Suga, H and Fujimoto, T (1986). Attraction of chick primordial germ cells by gonadal anlage in vitro. Anat. Rec., 215: 403-406.
Man, YG and Tavassoli, FA (1996). A simple epitope retrieval method without the use of microwave oven or enzyme digestion. Appl. Immunohistochem., 4: 139-141.
Meyer, DB (1960). Application of the periodic acid-schiff technique to whole chick embryos. Stain Technol., 35: 83-89.
Nagai, H; Mak, SS; Weng, W; Nakaya, Y; Ladher, R and Sheng, G (2011). Embryonic development of the emu, Dromaius novaehollandiae. Dev. Dyn., 240: 162-175.
Nye, JS; Kopan, R and Axel, R (1994). An activated Notch suppresses neurogenesis and myogenesis but not gliogenesis in mammalian cells. Development. 120: 2421-2430. | ||
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