| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,765 |
| تعداد دریافت فایل اصل مقاله | 11,580,455 |
Identification of the classical enterotoxin genes of Staphylococcus aureus in various foods by multiplex PCR assay | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 8، دوره 20، شماره 3 - شماره پیاپی 68، 2019، صفحه 209-212 اصل مقاله (308.86 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2019.5354 | ||
| نویسندگان | ||
| A. Mahfoozi1؛ H. Shirzad-Aski2؛ H. Kaboosi3؛ E. A. Ghaemi* 4 | ||
| 1Graduated from Biology Department, Damghan Branch, Islamic Azad University, Damghan, Iran | ||
| 2Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 3Department of Microbiology, College of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran | ||
| 4Food, Drug, and Natural Products Health Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran | ||
| چکیده | ||
| Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase chain reaction (PCR) assay. Methods: In order to achieve these goals, 310 samples, divided into three groups (dairy products, meat, and traditional sweets groups), were collected. After determination of the prevalence of S. aureus, the existence of 16s rRNA, sea, seb, sec, sed, and see genes were evaluated using multiplex PCR assay. Results: Staphylococcus aureus was isolated from 103 (33%) samples. Furthermore, the meat category had the most contamination rate of S. aureus. Additionally, the kebab samples (61.5%) were the most contaminated products, followed by hamburger (47.3%), and ice cream (33.8%). Of these 103 S. aureus isolates, 72 isolates (69.9%) harbored at least one type of the classical SEs genes. The prevalence of the type A enterotoxin gene was detected higher than the other SEs genes. Conclusion: The results indicated that inappropriate handling of the samples in the preparation and processing steps, especially for the meat products, can lead to the spread of more bacteria. The relatively high prevalence of some classical enterotoxin genes in the isolates revealed the potential power of this bacterium to produce enterotoxins, which can lead to food-poisoning. | ||
| کلیدواژهها | ||
| Enterotoxin؛ Food؛ Polymerase chain reaction؛ Staphylococcus aureus | ||
| مراجع | ||
|
Angulo, F; Beers, M; Cahill, S; Cowden, J; Davis, H; Desenclos, J; Edwards, L; Etzel, R; Galanis, E; Lynch, M; Marcus, R; McCarthy, N; Mitchell, R; Rocourt, J and Stehr-Green, J (2008). Foodborne disease outbreaks: guidelines for investigation and control. WHO Press. PP: 1-162.
Argudín, MA; Mendoza, MC; González-Hevia, MA; Bances, M; Guerra, B and Rodicio, MR (2012). Genotypes, exotoxin gene content, and antimicrobial resistance of Staphylococcus aureus strains recovered from foods and food handlers. Appl. Environ. Microbiol., 78: 2930-2935.
Argudín, MÁ; Mendoza, MC and Rodicio, MR (2010). Food poisoning and Staphylococcus aureus enterotoxins. Toxins (Basel). 2: 1751-1773.
Chaibenjawong, P and Foster, SJ (2011). Desiccation tolerance in Staphylococcus aureus. Arch. Microbiol., 193: 125-135.
Imani Fooladi, A; Tavakoli, HR and Naderi, A (2010). Detection of enterotoxigenic Staphylococcus aureus isolates in domestic dairy products. Iran. J. Microbiol., 2: 135-140.
ISO 6888-1 (1999). Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Technique using Baird-Parker agar medium. Int. Organ. Stand. Geneva.
Jay, JM; Loessner, MJ and Golden, DA (2005). Staphylococcal gastroenteritis. In: Modern food microbiology. Food Science Text Series. (7th Edn.), Springer US, Boston, MA., PP: 545-566. https://doi.org/10.1007/0-387-23413-6_23.
Kamarehei, F; Ghaemi, EA and Dadgar, T (2013). Prevalence of enterotoxin a and b genes in Staphylococcus aureus isolated from clinical samples and healthy carriers in Gorgan City, North of Iran. Indian J. Pathol. Microbiol., 56: 265.
Kraushaar, B; Ballhausen, B; Leeser, D; Tenhagen, BA; Käsbohrer, A and Fetsch, A (2017). Antimicrobial resistances and virulence markers in Methicillin-resistant Staphylococcus aureus from broiler and turkey: a molecular view from farm to fork. Vet. Microbiol., 200: 25-32. doi:10.1016/j.vetmic.2016.05.022.
Le Loir, Y; Baron, F and Gautier, M (2003). Staphylococcus aureus and food poisoning. Genet. Mol. Res., 2: 63-76.
Løvseth, A; Loncarevic, S and Berdal, KG (2004). Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates. J. Clin. Microbiol., 42: 3869-3872.
Moraveji, Z; Tabatabaei, M; Shirzad-Aski, H and Khoshbakht, R (2014). Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, southern Iran. Iran. J. Vet. Res., 15: 326-330.
Novick, RP; Schlievert, P and Ruzin, A (2001). Pathogenicity and resistance islands of staphylococci. Microbes Infect., 3: 585-594.
Pelisser, MR; Klein, CS; Ascoli, KR; Zotti, TR and Arisi, ACM (2009). Ocurrence of Staphylococcus aureus and multiplex PCR detection of classic enterotoxin genes in cheese and meat products. Brazilian J. Microbiol., 40: 145-148.
Peyravii, F; Kasra Kermanshahi, R; Ataee Ramezan, A; Akhavan Sepahi, A and Ghaemi, EA (2013). Molecular frequency of enterotoxigenic Staphylococcus aureus among different isolated of food products. Health Med., 7: 2105-2119.
Rall, VLM; Vieira, FP; Rall, R; Vieitis, RL; Fernandes, A; Candeias, JMG; Cardoso, KFG and Araújo, JP (2008). PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet. Microbiol., 132: 408-413. Santos, VM; Martins, HB; Rezende, IS; Barbosa, MS; Andrade, EF; Souza, SG; Campos, GB; Oliveira, PS; Sousa, DS; Silva, DCC; Da Amorim, AT; Timenetsky, J; Cruz, MP; Yatsuda, R and Marques, LM (2014). Virulence factor profile of Staphylococcus aureus isolated from bovine milk from Brazil. Food Nutr. Sci., 05: 1496-1505.
Seyoum, ET; Mekonene, TK; Woldetsadik, DA; Zewudie, BM and Gebreyes, WA (2016). Enterotoxin gene profile of Staphylococcus aureus isolates recovered from bovine milk produced in central Ethiopia. J. Infect. Dev. Ctries., 10: 138. doi:10.3855/jidc.6797.
Tang, J; Zhang, R; Chen, J; Zhao, Y; Tang, C; Yue, H; Li, J; Wang, Q and Shi, H (2015). Incidence and characterization of Staphylococcus aureus strains isolated from food markets. Ann. Microbiol., 65: 279-286. doi:10.1007/s13213-014-0859-2.
Wang, W; Baloch, Z; Jiang, T; Zhang, C; Peng, Z; Li, F; Fanning, S; Ma, A and Xu, J (2017). Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China. Front. Microbiol., 8: 1-11.
Wijesurendra, DS; Chamings, AN; Bushell, RN; Rourke, DO; Stevenson, M; Marenda, MS; Noormohammadi, AH and Stent, A (2017). Pathological and microbiological investigations into cases of bacterial chondronecrosis and osteomyelitis in broiler poultry. Avian Pathol., 46: 683-694. | ||
|
آمار تعداد مشاهده مقاله: 1,165 تعداد دریافت فایل اصل مقاله: 565 |
||