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Novel Isatin-based activator of p53 transcriptional functions in tumor cells | ||
| Molecular Biology Research Communications | ||
| مقاله 4، دوره 8، شماره 3، 2019، صفحه 119-128 اصل مقاله (799.47 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2019.34179.1419 | ||
| نویسندگان | ||
| Regina Mirgayazova1؛ Raniya Khadiullina1؛ Rimma Mingaleeva1؛ Vitaly Chasov1؛ Marina Gomzikova1؛ Ekaterina Garanina1؛ Albert Rizvanov1؛ Emil Bulatov* 1، 2 | ||
| 1Kazan Federal University, Kazan, Russian Federation | ||
| 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation | ||
| چکیده | ||
| Bioinorganic medicinal chemistry remains a hot field for research aimed at developing novel anti-cancer treatments. Discovery of metal complexes as potent antitumor chemotherapeutics such as cisplatin led to a significant shift of focus toward organometallic/ bioinorganic compounds containing transition metals and their chelates as novel scaffolds for drug discovery. In that way, transition metal complexes coordinated to essential biological scaffolds represent a highly promising class of compounds for design of novel target-specific therapeutics. Here, we report novel data on p53 activating Isatin-based Cu(II) complex exhibiting cytotoxic properties towards HCT116 and MCF7 tumor cell lines, as confirmed by cell viability assay and flow cytometry analysis of apoptosis. Furthermore, putative p53-mediated mechanism of action of this compound is supported by quantitative analysis of TP53, MDM2 and PUMA genes expression, as well as luciferase-based p53 pathway activation assay. Multiplex immunoassay analysis of inflammatory markers revealed potential modulation of several cytokines and chemokines. | ||
| کلیدواژهها | ||
| p53؛ Transcriptional activity؛ Isatin-Schiff base؛ Metal complex؛ Tumor cells | ||
| مراجع | ||
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